Elucidating the measurement of telomeres: Development of a transdisciplinary, high-impact Telomere Research Network

阐明端粒的测量：建立跨学科、高影响力的端粒研究网络

• 批准号: 10643990
• 负责人: Stacy Schmidt Drury
• 金额: $ 71.33万
• 依托单位: TULANE UNIVERSITY OF LOUISIANA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-30 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10643990
• 关键词: Acceleration; Address; Advisory Committees; Aging; Agreement; Air Pollution; Basic Science; Biological; Biological Assay; Biological Markers; Biometry; Cardiovascular Diseases; Clinical Research; Collaborations; Data; Databases; Dedications; Development; Discipline; Disease; Disease susceptibility; Educational workshop; Ensure; Environmental Exposure; Evaluation; Explosion; Fostering; Future; Goals; Grant; Health; Individual; Infrastructure; Investments; Laboratories; Length; Life; Life Cycle Stages; Link; Longevity; Measurement; Measures; Mental Depression; Methodology; Methods; Modeling; National Institute of Environmental Health Sciences; Onset of illness; Outcome; Pathway interactions; Persons; Pilot Projects; Population; Population Study; Predictive Value; Procedures; Process; Protocols documentation; Psychosocial Stress; Reporting; Reproducibility; Research; Research Personnel; Risk; Sampling; Schizophrenia; Scientific Advances and Accomplishments; Scientist; Sentinel; Shapes; Stress; Telomerase; Testing; Training; Translational Research; Validation; age related; biological specimen archives; cohort; disorder risk; early life exposure; early life stress; expectation; healthspan; innovation; interest; meetings; mortality; next generation; novel; population based; prevention effectiveness; psychosocial; recruit; research study; sample collection; telomere; virtual; web site

PROJECT SUMMARY The proposed Telomere Research Network (TRN) will establish best practices related to telomere length (TL) measurement applicable to population-based studies and will define the extent to which TL can be effectively applied as a sentinel of aging-related disease risk and indicator of environmental and psychosocial stress exposure across the life span. Across scientific fields, the use of telomeres as a marker of exposure and a harbinger of disease risk and accelerated aging is expanding. The explosion of telomere research, while promising, has raised methodological and reporting concerns triggered by inconsistent reproducibility. To address these concerns and advance telomere research, this U24 proposes the following specific AIMS: 1) Create a living network of invested stakeholders to address challenges in determining the extent to which TL is a sentinel of environmental exposures, psychosocial stress, and disease susceptibility; and to ensure the highest rigor and reproducibility in current and future telomere studies. 2) Support and coordinate U01 efforts by ensuring seamless sharing of samples, data, and protocols and fostering interactive discussions focused on best practices for TL measurement. 3) Facilitate the infrastructure for sustained cross-discipline dialogue, dissemination, and research related to TL as a sentinel of cumulative exposure and a predictor of health and aging. To achieve these AIMS, the U24 proposes to recruit a diverse and interactive network of scientists invested in resolving methodologic concerns in TL metrics; engage in formal testing of the extent to which TL, telomerase and other telomere indicators serve as sentinels of age-related disease and cumulative exposures; and define best practices for TL measured in population studies. These AIMS will produce clear methodologic standards, pipelines for increased cross-study analyses and collaboration, and a sustainable platform for continued dialogue related to the measurement of TL and its implications for aging, exposure, and stress research.

项目概要 拟议的端粒研究网络（TRN）将建立与端粒长度（TL）相关的最佳实践 适用于基于人群的研究的测量，并将定义 TL 作为一种方法可以有效应用的程度 与衰老相关的疾病风险的哨兵以及整个生命周期环境和心理社会压力暴露的指标。 在整个科学领域，使用端粒作为暴露标记以及疾病风险和加速衰老的预兆 正在扩大。端粒研究的爆炸式增长虽然充满希望，但也引起了方法论和报告方面的担忧 由不一致的再现性引发。为了解决这些问题并推进端粒研究，本 U24 提出 遵循具体目标： 1) 创建一个由投资利益相关者组成的活跃网络，以应对确定投资目标的挑战 TL 在多大程度上是环境暴露、社会心理压力和疾病易感性的哨兵；并确保 当前和未来端粒研究中最高的严谨性和可重复性。 2) 支持和协调 U01 的工作 确保样品、数据和协议的无缝共享，并促进以最佳实践为重点的互动讨论 用于 TL 测量。 3) 促进持续跨学科对话、传播和研究的基础设施 TL 作为累积暴露的哨兵以及健康和衰老的预测因子。为了实现这些目标，U24 提议招募一个多元化、互动的科学家网络，致力于解决 TL 的方法学问题 指标；对 TL、端粒酶和其他端粒指标作为哨兵的程度进行正式测试 与年龄相关的疾病和累积暴露；并定义人口研究中测量 TL 的最佳实践。这些 AIMS 将制定明确的方法标准、用于增加交叉研究分析和协作的管道，以及 持续对话的可持续平台，涉及 TL 测量及其对老龄化、暴露和健康的影响 压力研究。

项目成果

Monochrome Multiplex Quantitative PCR Telomere Length Measurement.

单色多重定量 PCR 端粒长度测量。

• DOI: 10.3791/66545
• 发表时间: 2024
• 期刊: Journal of visualized experiments : JoVE
• 作者: Martin,NoelleA;McLester-Davis,LaurenWY;Roy,TrevorR;Magruder,MadelineG;Hastings,WaylonJ;Drury,StacyS
• 通讯作者: Drury,StacyS

Method comparison studies of telomere length measurement using qPCR approaches: A critical appraisal of the literature.

• DOI: 10.1371/journal.pone.0245582
• 发表时间: 2021
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Lindrose AR;McLester-Davis LWY;Tristano RI;Kataria L;Gadalla SM;Eisenberg DTA;Verhulst S;Drury S
• 通讯作者: Drury S