Photothermal Nanocomposites for Tissue Repair

用于组织修复的光热纳米复合材料

• 批准号: 8945323
• 负责人: Kaushal Rege
• 金额: $ 38.16万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-09 至 2019-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8945323
• 关键词: American; Anastomosis - action; Bacteria; Biochemical; Biocompatible; Biocompatible Materials; Clinical; Collagen; Colon; Colorectal; Colorectal Cancer; Colorectal Surgery; Deposition; Development; Drug Delivery Systems; Drug usage; Elastin; Encapsulated; Evaluation; Excision; Extravasation; Family suidae; Fibrin; Generations; Goals; Gold; Heating; Human; Immune response; Inflammatory Bowel Diseases; Intestines; Laser injury; Lasers; Lead; Light; Liquid substance; Manuscripts; Matrix Metalloproteinase Inhibitor; Measurement; Mechanics; Mediating; Medicine; Methods; Modeling; Morbidity - disease rate; Operative Surgical Procedures; Pharmaceutical Preparations; Physiologic pulse; Prevention; Procedures; Property; Proteins; Recovery; Reporting; Research; Rupture; Surgical Anastomosis; Surgical incisions; Surgical sutures; Tensile Strength; Time; Tissue Model; Tissues; Translations; United States; Welding; base; biomacromolecule; biomaterial compatibility; clinical application; gastrointestinal; improved; in vivo; irradiation; mortality; mouse model; nanocomposite; nanorod; news; novel; polypeptide; pressure; prevent; public health relevance; repaired; response; seal; small molecule; tissue repair; tissue welding

 DESCRIPTION: The ability of photothermal biomaterials to convert light to heat can have diverse applications in medicine. However, the lack of effective biomaterials has adversely impacted progress and clinical translation in this promising field. The overall goal of this proposed research is to generate, characterize, and apply photothermal nanocomposites for effective tissue repair. In this approach, we will generate nanocomposites in which, gold nanorods are incorporated within biocompatible biomacromolecules including, elastin-like polypeptides (ELPs), collagen, fibrin and their blends. The photothermal response of these nanocomposites will be investigated using both, pulsed and continuous wavelength lasers at different power intensities. In addition, the mechanical properties and efficacy of laser-triggered drug (MMP inhibitor) delivery from these nanocomposites will be determined (Specific Aim 1). Nanocomposites with effective photothermal properties will be investigated for their ability to repair ruptured porcine intestinal tissue ex vivo; an incision model of tissue rupture will be investigated. Both pulsed and continuous lasers, at different power intensities, will be investigated in order to facilitate nanocomposite-mediated localized photothermal welding of the ruptured tissue. Tensile strength, leak pressure and burst pressure will be investigated in order to determine the mechanical integrity of the nanocomposite-repaired tissue. The efficacy of photothermal nanocomposites for preventing bacterial leakage from bacteria-rich intestinal lumen will also be investigated. Nanocomposite composition and operating conditions that result in the most effective recovery of tissue properties compared to intact tissue will be identified (Specific Aim 2). The most effective nanocomposite type and operating conditions will be further evaluated for maintaining tissue integrity and facilitating repair in a mouse model of colon leakage. Mechanical properties, including burst pressures, biochemical and immune responses, effect of MMP inhibitor delivery, and photothermal effects will be determined following in vivo welding. Longer-term survival studies will also be carried out (Specific Aim 3). It is anticipated that the current research will lead to transformative developments in both, fundamental studies as well as application of biocompatible photothermal nanocomposites for tissue repair. Findings from this research have very high potential for direct translation to several clinical applications that can benefit from the several advantages of photothermal tissue repair.

 描述：光热生物材料将光转化为热的能力可以在医学上有多种应用，但是，缺乏有效的生物材料对这一前景广阔的领域的进展和临床转化产生了不利影响。 ，并应用光热纳米复合材料进行有效的组织修复在这种方法中，我们将生成纳米复合材料，其中金纳米棒被纳入生物相容性生物大分子中，包括弹性蛋白样多肽。 （ELP）、胶原蛋白、纤维蛋白及其混合物将使用不同功率强度的脉冲和连续波长激光进行研究。此外，还将研究激光触发的机械性能和功效。 将确定这些纳米复合材料的药物（MMP 抑制剂）输送（具体目标 1）将研究具有有效光热特性的纳米复合材料离体修复破裂猪肠组织的能力；将研究组织破裂的切口模型。将研究不同功率强度的连续激光，以促进纳米复合材料介导的破裂组织的局部光热焊接的拉伸强度、泄漏压力。还将研究光热纳米复合材料在防止细菌从富含细菌的肠腔中渗漏的功效和爆裂压力，以确定纳米复合材料修复组织的机械完整性。将确定与完整组织相比组织特性的有效恢复（具体目标 2）将进一步评估最有效的纳米复合材料类型和操作条件，以维持组织完整性并促进小鼠结肠模型的修复。机械性能，包括爆破压力、生化和免疫反应、MMP 抑制剂传递的影响以及光热效应也将在体内焊接后进行确定（具体目标 3）。当前的研究将带来基础研究以及生物相容性光热纳米复合材料在组织修复中的应用的变革性发展，该研究的结果具有直接转化为多种临床应用的巨大潜力。 这可以受益于光热组织修复的几个优点。