Role of Distortion Energy in Fibroblast-Mediated Remodeling of Collagen Matrices

畸变能在成纤维细胞介导的胶原基质重塑中的作用

基本信息

批准号：
10452423
负责人：
Trevor Justin Lujan
金额：
$ 11.35万
依托单位：
BOISE STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-23 至 2024-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10452423
关键词：
3-Dimensional Adopted Adoption Algorithms Anisotropy Awareness Behavior Biological Biomechanics Cells Chemicals Collagen Communities Computer software Confocal Microscopy Congresses Cytoskeleton Dense Connective Tissue Dependence Devices Documentation Educational workshop Engineering Ensure Extracellular Matrix Fiber Fibroblasts Fluorescence Microscopy Future Growth Image Intuition Laboratories Laboratory Research Length Liver Manuals Mathematics Measurement Measures Mechanics Mediating Molecular Muscle Notification Operating System Optics Parents Periodicity Property Publications Pythons Research Research Project Grants Role Running Science Software Engineering Software Tools Structure Structure-Activity Relationship Students System Testing Textiles Tissues Universities Validation base biological systems cloud based design engineering design image processing imaging modality improved light microscopy materials science mechanical behavior microscopic imaging muscle engineering preference repaired scaffold soft tissue software development spatiotemporal symposium tool tumor two-dimensional

项目摘要

PROJECT SUMMARY Dense connective tissue is composed of an abundant collagen network that is maintained and repaired by fibroblasts. The parent R15 proposal is investigating whether fibroblast-mediated collagen remodeling is governed at the tissue-scale by distortion strain energy. The effect of targeted magnitudes of distortion energy on collagen remodeling will be measured by quantifying alterations in the composition, organization, and mechanical behavior of the scaffolds. In order to quantify changes in fiber organization from confocal microscopy images, a software application called FiberFit is being used to automate the objective measurement of two structural properties that describe material anisotropy: fiber orientation and fiber dispersion. The standalone software application, FiberFit, was developed in the Northwest Tissue Mechanics laboratory in 2016 and is freely available to the scientific community for Windows and Mac systems. FiberFit has now been used in many research projects outside the developing laboratory, including research on tumors, liver, muscle, and engineered composite materials. This project will re-engineer FiberFit and deploy it as a cloud-based application. Emphasis will be placed on creating a robust, intuitive, and sustainable software application that implements best practices in software engineering and design to facilitate broad adoption across the scientific community.

项目摘要密集的结缔组织由一个丰富的胶原蛋白网络组成，该网络由维持和修复成纤维细胞。家长R15提案正在研究成纤维细胞介导的胶原蛋白重塑是否为通过失真应变能在组织尺度上控制。靶向失真能量的效果胶原蛋白重塑将通过量化组成，组织和支架的机械行为。为了量化聚焦的光纤组织的变化显微镜图像，一种称为FiberFit的软件应用程序用于自动化客观测量描述材料各向异性的两个结构特性：纤维方向和纤维分散。这独立软件应用程序FiberFit于2016年在西北组织力学实验室开发并且可以免费提供Windows和Mac系统的科学界。现在使用了Fiberfit 在发展实验室以外的许多研究项目中，包括肿瘤，肝脏，肌肉和工程复合材料。该项目将重新设计纤维纤维并将其部署为基于云的应用。重点将放在创建强大，直观和可持续的软件应用程序上在软件工程和设计中实施最佳实践，以促进科学的广泛采用社区。