The Resource of the MMRRC at UC Davis

加州大学戴维斯分校 MMRRC 的资源

基本信息

批准号：
10341115
负责人：
KC KENT LLOYD
金额：
$ 119.88万
依托单位：
UNIVERSITY OF CALIFORNIA AT DAVIS
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-09-30 至 2025-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10341115
关键词：
Adopted Advertising Alleles Analgesics Applied Research Archives Area Awareness Bacterial Artificial Chromosomes Biomedical Research Biotechnology CRISPR/Cas technology California Caring Categories Collection Communities Contracts Cost efficiency Country Cryopreservation Custom Database Management Systems Development Disease Diverse Workforce ES Cell Line Effectiveness Embryo Ensure Environment Equipment Ethylnitrosourea Evaluation Feedback Freedom Funding Gene Targeting Genomics Genotype Goals Health Human Resources Individual Informatics Information Dissemination Infrastructure Injections Institutes Institution Intelligence Intracytoplasmic Sperm Injections Knock-out Leadership Marketing Mentors Mission Modeling Molecular Analysis Mus Mutant Strains Mice National Heart, Lung, and Blood Institute Outcome Performance Phenotype Policies Positioning Attribute Practice Guidelines Prevention Procedures Protocols documentation Published Comment Recommendation Reproducibility Research Research Activity Research Personnel Research Project Grants Resource Development Resources Role Scientist Security Services Speed Technology Time Training Transgenic Organisms United States National Institutes of Health Universities Work biobank biomedical resource blastocyst community engaged research congenic embryonic stem cell genetic variant genome editing gut microbiome high standard improved innovation knockout gene meetings microbiome analysis microbiota transplantation mouse model multiple omics mutant mutant mouse model next generation online resource operation pathogen programs repository tool web site zygote

项目摘要

ABSTRACT: Resource Section This proposal seeks renewal of 5 year support for the infrastructure, personnel, and resources of the Mutant Mouse Resource and Research Center (MMRRC) at the University of California (UC) Davis (MMRRC-UCD). Since its inception 20 years ago, the MMRRC-UCD has been one of the primary mutant mouse archive and distribution repositories in the world. As the largest of the 4 Centers in the MMRRC Consortium, the MMRRC- UCD has assumed a position of leadership in innovation and resource development. The MMRRC-UCD has introduced new products (e.g., KOMP knockout first alleles in ES cells, CRISPR/Cas9 genome edited alleles in zygotes) and initiated new services (e.g., blastocyst injection, ICSI, speed congenics, genetic variant modeling, microbiome analysis) that have added scientific value to mouse models submitted to the MMRRC, which in turn has enhanced utilization of the MMRRC by the scientific research community. As a result, to date the MMRRC-UCD leads the Consortium in the number of alleles archived (50,040 out of 59,847), orders (6,293 out of 13,165), and investigators served (4,372 out of 6477) at 2,445 institutions in 23 countries. The MMRRC- UCD has leveraged its infrastructure and expertise to extend its service role by contracting with categorical NIH institutes (e.g., NHLBI BayGenomics project), biotechnology companies (Genentech), and several organizations (e.g., the BaSH Consortium) to archive, distribute, custom breed, genotype, and phenotype 1000’s of transgenic and knockout, gene-targeted ES cell lines, BAC-transgenic, CRE, ENU-induced, and other mutant mouse collections. In addition, the MMRRC-UCD has worked with its MMRRC Consortium partners and NIH program staff to develop and implement advertising and marketing strategies, database management and informatics applications, website and online resources, customer and user services, operating procedures and protocols, and policies and practicies for shared governance. Further, over the last several years, the MMRRC-UCD completed several research projects in multiple areas that have enhanced operations of the resource, including in cryopreservation, gut microbiome analysis, mouse analgesics administration, genetic variant modeling using CRISPR/Cas9, and more. Over the next 5 years, the MMRRC- UCD looks forward to the assignment of another ~250 mouse lines (Type 1), providing users access to several new services including Fast-Track Genome Editing and Genomic Integration Analysis, and conducting 3 new research projects in the areas of microbiome transplantation, quantitative embryo evaluation, and multi-omic molecular analyses. By archiving and delivering research mouse products and services effectively and efficiently, and by conducting applied research activities to improve operations of the resource, the MMRRC- UCD aims to continue as a scientifically valuable biorepository for the biomedical research community. Over the next 5 years, we shall focus our efforts on optimizing the quality of our resource, ensuring its relevance to individual users, and seeking maximum performance for the benefit of all whom we serve.

摘要：资源部分该提案寻求对突变体的基础设施、人员和资源延长 5 年支持加州大学 (UC) 戴维斯分校小鼠资源和研究中心 (MMRRC) (MMRRC-UCD)。自 20 年前成立以来，MMRRC-UCD 一直是主要的突变小鼠档案库之一，作为 MMRRC 联盟 4 个中心中最大的一个，MMRRC- MMRRC-UCD 在创新和资源开发方面处于领先地位。推出新产品（例如，KOMP 敲除 ES 细胞中的第一个等位基因、CRISPR/Cas9 基因组编辑的等位基因受精卵）并启动了新服务（例如囊胚注射、ICSI、速度同源、遗传变异建模、微生物组分析）为提交给 MMRRC 的小鼠模型增加了科学价值，结果，迄今为止，科学研究界对 MMRRC 的利用得到了提高。 MMRRC-UCD 在存档的等位基因数量（59,847 个中的 50,040 个）、订单数（59,847 个中的 6,293 个）方面领先联盟。 13,165 名），调查人员（6477 名中的 4,372 名）在 23 个国家的 2,445 个机构中服务。都柏林大学利用其基础设施和专业知识，通过与分类机构签订合同来扩展其服务角色 NIH 机构（例如 NHLBI BayGenomics 项目）、生物技术公司（Genentech）和一些组织（例如 BaSH 联盟）存档、分发、定制品种、基因型和表型 1000 个转基因和敲除、基因靶向 ES 细胞系、BAC 转基因、CRE、ENU 诱导和此外，MMRRC-UCD 还与其 MMRRC 联盟合作。合作伙伴和 NIH 项目人员制定和实施广告和营销策略、数据库管理和信息学应用程序、网站和在线资源、客户和用户服务、此外，在过去，共享治理的操作程序和协议、政策和实践。几年来，MMRRC-UCD 完成了多个领域的多个研究项目，这些项目增强了资源的操作，包括冷冻保存、肠道微生物组分析、小鼠镇痛药管理、使用 CRISPR/Cas9 进行遗传变异建模等，在未来 5 年内，MMRRC- UCD 期待分配另外约 250 条鼠标线（类型 1），为用户提供多种访问方式新服务包括快速基因组编辑和基因组整合分析，并开展 3 项新服务微生物组移植、定量胚胎评估和多组学领域的研究项目通过有效地归档和提供研究小鼠产品和服务，有效地，并通过开展应用研究活动来改善资源的运营，MMRRC- 都柏林大学的目标是继续成为生物医学研究界具有科学价值的生物样本库。未来5年，我们将集中精力优化资源质量，确保其与个人用户，并为我们所服务的所有人的利益寻求最佳性能。