Research Support Core: Analytical Core

研究支持核心：分析核心

• 批准号: 8451614
• 负责人: LARRY W ROBERTSON
• 金额: $ 32.99万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-12 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8451614
• 关键词: Animals; Architecture; Archives; Aroclor; Aroclors; Blood; Calibration; Collaborations; Data Reporting; Databases; Development; Equipment; Human; Human Resources; Individual; Injection of therapeutic agent; Laboratories; Laboratory Animals; Maintenance; Methods; Performance; Polychlorinated Biphenyls; Protocols documentation; Quality Control; Recovery; Reference Standards; Research; Research Personnel; Research Support; Sampling; Services; Solutions; Source; System; Testing; Toxic effect; Toxicology; Training; Universities; base; design; instrumentation; programs; quality assurance

The Analytical Core provides routine and non-routine analytical service to the University of lowa research program. The core provides expert staff, equipment, and instrumentation in line with five Aims. Specific Aim 1 is the development of analytical and quality control/assurance standards. In close collaboration with the Synthesis Core, which is responsible for acquiring and archiving individual compound standards and preparing stock solutions, the Analytical Core develops the mathematical methods for congener representation, analyzes single congener solutions for purity assessment and quality control, and tests the prepared standard solutions against certified Standard Reference Materials, including PCB calibration mixtures. Specific Aim 2 is the development of a mixture of PCB congeners for laboratory animal exposure studies. The Analytical Core develop the mathematical methods to design the exposure mixture and tests the prepared mixtures against certified Standard Reference Materials. Specific Aim 3 is the analysis of environmental and laboratory samples for a suite of PCB congener. The Analytical Core will extract and analyze PCBs on a congener-specific basis: 209 congeners are detected as individual or coeluting sets. We typically achieve 170 congener separations in environmental samples. Applying the primary calibration standard developed under Aim 1, samples are quantified by the internal standard method. Internal Standards and Surrogate Recovery Standards developed under Aim 1 are used to precisely determine PCB concentrations in samples. Methods for analysis on a congener-specific basis are assessed using performance standard injections and analysis of certified Standard Reference Materials. Most analyses are performed on the entire suite of PCB congeners, including laboratory animals exposed to the mixtures described in Aim 2 and including blood from humans and non-laboratory animals. Other samples are analyzed for specific congeners and metabolites in support of investigators focused on congener-specific toxicology. Specific Aim 4 involves the extraction, identification, and quantification of unknown metabolites. The Analytical Core determines purity of synthesized metabolites and associated analytical standards, then analyzes for hydroxylated PCBs and other PCB metabolites. As requested by the isbrp investigators, the Analytical Core will also train personnel and assist in sampling design and implementation. Specific Aim 5 is the design and maintenance of a database system. A redundant storage architecture with automatic backup and password protection is used to allow investigators to download data and reports produced by the Analytical Core.

分析核心为洛瓦大学研究项目提供常规和非常规分析服务。该核心根据五个目标提供专家人员、设备和仪器。具体目标 1 是制定分析和质量控制/保证标准。分析核心与负责获取和归档单个化合物标准品并准备库存解决方案的合成核心密切合作，开发同系物表示的数学方法，分析单个同系物解决方案以进行纯度评估和质量控制，并测试准备好的标准品针对经过认证的标准参考材料（包括 PCB 校准混合物）的解决方案。具体目标 2 是开发用于实验动物暴露研究的 PCB 同系物混合物。分析核心开发数学方法来设计暴露混合物，并根据经过认证的标准参考材料测试制备的混合物。具体目标 3 是对一套 PCB 同系物的环境和实验室样品进行分析。分析核心将在特定同系物的基础上提取和分析 PCB：209 种同系物被检测为单独或共洗脱组。我们通常在环境样品中实现 170 个同系物分离。应用目标 1 下开发的主要校准标准，样品 采用内标法定量。根据目标 1 开发的内标和替代回收标准用于精确测定样品中的 PCB 浓度。使用性能标准进样和经过认证的标准参考材料的分析来评估同类物特定分析方法。大多数分析都是针对整套 PCB 同系物进行的，包括暴露于目标 2 中描述的混合物的实验动物以及来自人类和非实验动物的血液。对其他样品进行特定同系物和代谢物分析，以支持专注于同系物特异性毒理学的研究人员。具体目标 4 涉及提取、鉴定和量化 未知的代谢物。分析核心确定合成代谢物的纯度和相关分析标准，然后分析羟基化 PCB 和其他 PCB 代谢物。根据 isbrp 调查人员的要求，分析核心还将培训人员并协助抽样设计和实施。具体目标5是数据库系统的设计和维护。具有自动备份和密码保护功能的冗余存储架构用于允许调查人员下载分析核心生成的数据和报告。