Molecular and Cellular Biology Core

分子和细胞生物学核心

• 批准号: 10397674
• 负责人: Philip Ross Hardwidge
• 金额: $ 27.06万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-06-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10397674
• 关键词: Advisory Committees; Animal Model; Area; Biochemical; Biochemistry; Bioinformatics; Biological Assay; Biology; Biomedical Research; Biophysics; CRISPR/Cas technology; Cell Separation; Centers of Research Excellence; Clustered Regularly Interspaced Short Palindromic Repeats; Collection; Committee Members; Communicable Diseases; Communities; Core Facility; DNA sequencing; Data; Data Set; Diagnostic; Doctor of Philosophy; Electron Microscopy; Employee; Environment; Equipment; Extramural Activities; Faculty; Financial Support; Flow Cytometry; Fluorescence Microscopy; Funding; Goals; Growth; Image Cytometry; Imaging technology; Individual; Infrastructure; Kansas; Laboratories; Leadership; Maintenance; Mentors; Methodology; Modernization; Molecular; Molecular and Cellular Biology; Pathogenesis; Pathology; Performance; Postdoctoral Fellow; Research; Research Personnel; Research Project Grants; Resources; Seeds; Services; Teacher Professional Development; Techniques; Technology; Time; Training; Universities; Veterinary Medicine; Zoonoses; college; equipment acquisition; experience; experimental study; graduate student; improved; innovation; instrument; laser capture microdissection; member; next generation; pathogen; programs; single-cell RNA sequencing; synergism; undergraduate student; vaccine development

Project Summary: The Molecular and Cellular Biology (MCB) Core facility will be modernized and enhanced by adding new equipment and services to expand the capabilities of the facility. The MCB Core is a fully functional research core that currently provides imaging and flow cytometry services to CVM users. MCB Core facility improvements will focus on expanding the DNA sequencing, CRISPR technology, and cell sorting capabilities. Significant new financial support from Kansas State University (KSU) for new equipment acquisition and facility maintenance and sustainability has been obtained and will further expand the range of technologies available in the facility, providing cutting-edge technological support for current and future research projects. Dr. Philip R. Hardwidge, a bacterial pathogenesis expert, will serve as MCB Core director. Dr. Waithaka Mwangi, the current director of KSU CVM's flow cytometry facility and a vaccine development expert, will serve as MCB Core associate director, providing additional senior leadership. MCB Core support for bioinformatics and handling of large DNA sequencing datasets will be provided by the KSU Bioinformatics Center, under the direction of Dr. Sue Brown, Division of Biology. Expert technical support for DNA sequencing will be provided by Dr. Doug Marthaler, the current director of DNA sequencing in the KSU Veterinary Diagnostic Laboratory. Implementation and refinement of cutting-edge CRISPR technology will be provided by Dr. Greg Finnigan, in the KSU Biochemistry and Molecular Biophysics Department. Additional technological, project-specific advice and mentoring will be provided by the COBRE mentoring team, internal and external advisory committee members, and members from the CVM and Division of Biology at KSU. A senior research associate, Mike Hays, will provide day-to-day management of the facility, and will communicate with project leaders to identify specific, evolving needs for individual projects and the Core. Mike Hays possesses significant experience in the molecular and cellular biology methodologies that will be utilized. The Core has already established the feasibility of providing advanced imaging technology, next-generation DNA sequencing and CRISPR resources to junior project leaders. These activities will be tightly integrated with the Animal Model and Pathology (AMP) Core. The AMP core will also undergo technological upgrades, including the acquisition of a laser capture microdissection instrument. Significant synergy will exist between the two cores and will allow for the first time at KSU the performance of single-cell RNA-Seq experiments. The MCB Core will directly support the Center's primary and pilot/seed projects, as well as the community of users within KSU and the regional scientific network (RSN), including faculty, staff, postdocs, and graduate students. The significant enhancement of the MCB Core facilities will also provide an excellent training environment for the robust Ph.D. program at KSU. Most importantly, MCB Core upgrades will improve the overall infrastructure for biomedical research at KSU.

项目概要： 分子和细胞生物学 (MCB) 核心设施将通过增加新设施进行现代化和增强 设备和服务，以扩展设施的能力。 MCB 核心是一个功能齐全的研究 目前为 CVM 用户提供成像和流式细胞术服务的核心。 MCB 核心设施改进 将专注于扩展DNA测序、CRISPR技术和细胞分选能力。重大新 堪萨斯州立大学 (KSU) 对新设备购置和设施维护的财政支持 已经实现了可持续性，并将进一步扩大该设施可用的技术范围， 为当前和未来的研究项目提供尖端技术支持。菲利普·R·哈德威奇 (Philip R. Hardwich) 博士 细菌发病机制专家，将担任MCB核心主任。 Waithaka Mwangi 博士，现任主任 KSU CVM 的流式细胞仪设施和疫苗开发专家将担任 MCB Core 副主任， 提供额外的高级领导。 MCB Core 支持生物信息学和大 DNA 处理 测序数据集将由 KSU 生物信息学中心在 Sue Brown 博士的指导下提供， 生物学部。 DNA 测序的专家技术支持将由 Doug Marthaler 博士提供，他是 现任 KSU 兽医诊断实验室 DNA 测序主任。实施与细化 尖端 CRISPR 技术将由肯尼迪州立大学生物化学系的 Greg Finnigan 博士提供 分子生物物理学系。额外的技术、特定于项目的建议和指导将 由 COBRE 指导团队、内部和外部咨询委员会成员以及来自 堪萨斯州立大学的 CVM 和生物学系。高级研究员迈克·海斯 (Mike Hays) 将提供日常研究 设施管理，并将与项目负责人沟通，以确定具体的、不断变化的需求 个人项目和核心。 Mike Hays 在分子和细胞领域拥有丰富的经验 将使用的生物学方法。核心已经确立了提供先进技术的可行性 向初级项目负责人提供成像技术、下一代 DNA 测序和 CRISPR 资源。这些 活动将与动物模型和病理学（AMP）核心紧密结合。 AMP 核心还将 进行技术升级，包括购买激光捕获显微切割仪器。 两个核心之间将存在显着的协同作用，并将在 KSU 首次实现以下性能： 单细胞RNA测序实验。 MCB 核心将直接支持中心的初级和试点/种子 项目以及 KSU 和区域科学网络 (RSN) 内的用户社区，包括 教职员工、博士后和研究生。 MCB 核心设施的显着增强也将 为优秀的博士生提供良好的培养环境。 KSU 的项目。最重要的是，MCB 核心 升级将改善堪萨斯州立大学生物医学研究的整体基础设施。