TR&D3-SCH

TR

• 批准号: 10217179
• 负责人: TIMOTHY A CROSS
• 金额: $ 12.67万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217179
• 关键词: Biological; Biological Process; Biomedical Research; Cell Nucleus; Cellular Membrane; Chemistry; Chills; Complex; Coupled; Crystallization; Crystallography; Detection; Development; Dimensions; Feedback; Future; Goals; High temperature of physical object; Hybrids; Lead; Lipid Bilayers; Measurement; Membrane Proteins; Metals; Molecular Structure; Molecular Weight; NMR Spectroscopy; Noise; Oxygen; Power Sources; Proteins; Protons; Regulation; Relaxation; Residual state; Resolution; Resources; Sampling; Scientist; Series; Signal Transduction; Site; Spectrum Analysis; Speed; Structure; Technology; Temperature; Testing; Time; Work; biological systems; cold temperature; experimental study; frontier; improved; macromolecule; magnetic field; new technology; next generation; restraint; solid state nuclear magnetic resonance; structural biology; tool; water flow

TR&D3 - Project Summary/Abstract Significant increases in field strength have always led to new frontiers in the scientific arenas approachable by NMR spectroscopy. Often the challenges and what has been viewed as the drawbacks to high fields have proven to some of their greatest advantages. The Series Connected Hybrid (SCH) magnet, at a field of 36T, that will become operational in the summer of 2016 at the NHMFL will provide a view into the future for NMR spectroscopy; a future in which High Temperature Superconducting (HTS) magnets will have the potential for doubling the field strengths of current Low-Temperature superconducting (LTS) magnets. The SCH magnet is a hybrid magnet having an outer superconducting coil of 13T and inner stacks of Bitter plates that will be powered by 14MW and cooled by deionized water flowing through the stacks at 1700 gal/min. The SCH magnet will be the highest homogeneity and the highest stability resistive magnet that has been constructed, but the homogeneity and stability will not be the same as that for LTS magnets. The BTRR will develop a biomedical research focus on this magnet through the development of additional technology using a cross section of scientists committed to developing and pushing the scientific frontiers of NMR spectroscopy at a field strength that is more than 50% higher than any other NMR spectrometer in the world. More than a decade of effort has now gone into developing new technologies to enhance the stability and homogeneity of this magnet. Two approaches are being taken: first, an advanced lock unit has been developed by Bruker and tested at the NHMFL and secondly, Prof. Jeffrey Schiano from Penn State has developed a cascade field regulation technology that takes advantage of an inductive pickup coil as well as NMR measurements to provide a feedback field correction. A new 1H detection HXY 1.3 mm MAS probe will be developed for structural biology solid state NMR. 1H detection has the potential to revolutionize solid state NMR in much the same way 1H detection revolutionized solution NMR 30 years ago. This revolution will open will facilitate the structural characterization of membrane proteins in lipid bilayers and potentially in native cellular membrane. This technology will be coupled with oriented samples ssNMR that provides complementary structural restraints. The signal to noise per unit of spectrometer time for spin ½ nuclei improves with approximately B03, but for quadrupolar nuclei the rate of enhancement can be even greater due to a wide variety of factors including relaxation effects. While there are many odd halves quadrupolar nuclei in biological systems the most common and important one is 17O. Oxygen atoms are the primary sites in biological macromolecules of catalytic and functional chemistry. A goal in this effort is to work toward developing 17O spectroscopy as a routine spectroscopic tool.

TR&D3 - 项目摘要/摘要 场强的显着增加总是导致科学领域的新领域 通常可以通过 NMR 光谱来解决这些挑战和缺点。 高磁场已经证明了串联混合 (SCH) 磁铁的一些最大优势。 一个 36T 的场地将于 2016 年夏天在 NHMFL 投入使用，届时将提供对 核磁共振光谱学的未来；高温超导（HTS）磁体的未来 使当前低温超导（LTS）磁体的磁场强度加倍的潜力。 SCH 磁体是一种混合磁体，具有 13T 的外部超导线圈和内部的 Bitter 板堆叠 其功率为 14MW，并由以 1700 加仑/分钟流经烟囱的去离子水冷却。 SCH磁体将是迄今为止均匀性最高、稳定性最高的电阻磁体 结构，但均匀性和稳定性与 LTS 磁体不同。 通过使用磁体开发附加技术来开展生物医学研究 致力于开发和推动核磁共振波谱学科学前沿的科学家的横截面 场强比世界上任何其他核磁共振波谱仪高 50% 以上。 经过十多年的努力，现在已经投入到开发新技术以增强稳定性和 正在采取两种方法来提高这种磁铁的同质性：首先，开发了一种先进的锁定装置。 其次，来自宾夕法尼亚州立大学的 Jeffrey Schiano 教授开发了一种 利用感应拾波线圈和 NMR 的级联场调节技术 将使用新的 1H 检测 HXY 1.3 mm MAS 探头进行测量。 专为结构生物学固态 NMR 检测而开发，具有彻底改变固态的潜力。 NMR 以大致相同的方式 1H 检测彻底改变了 30 年前的 NMR 这场革命即将开启。 将促进脂质双层中膜蛋白的结构表征，并可能在天然中进行 该技术将与提供定向样品的 ssNMR 相结合。 互补的结构性约束。 自旋 1/2 核的每单位光谱仪时间的信噪比提高了大约 B03，但是 对于四极核，由于多种因素，增强率可能更大，包括 虽然生物系统中有许多奇数半四极核，但最常见的是。 其中重要的一个是17O，氧原子是生物大分子中催化和催化的主要位点。 这项工作的一个目标是致力于开发 17O 光谱作为常规。 光谱工具。