TR&D3-SCH

TR

• 批准号: 10217179
• 负责人: TIMOTHY A CROSS
• 金额: $ 12.67万
• 依托单位: FLORIDA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2023-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10217179
• 关键词: Biological; Biological Process; Biomedical Research; Cell Nucleus; Cellular Membrane; Chemistry; Chills; Complex; Coupled; Crystallization; Crystallography; Detection; Development; Dimensions; Feedback; Future; Goals; High temperature of physical object; Hybrids; Lead; Lipid Bilayers; Measurement; Membrane Proteins; Metals; Molecular Structure; Molecular Weight; NMR Spectroscopy; Noise; Oxygen; Power Sources; Proteins; Protons; Regulation; Relaxation; Residual state; Resolution; Resources; Sampling; Scientist; Series; Signal Transduction; Site; Spectrum Analysis; Speed; Structure; Technology; Temperature; Testing; Time; Work; biological systems; cold temperature; experimental study; frontier; improved; macromolecule; magnetic field; new technology; next generation; restraint; solid state nuclear magnetic resonance; structural biology; tool; water flow

TR&D3 - Project Summary/Abstract Significant increases in field strength have always led to new frontiers in the scientific arenas approachable by NMR spectroscopy. Often the challenges and what has been viewed as the drawbacks to high fields have proven to some of their greatest advantages. The Series Connected Hybrid (SCH) magnet, at a field of 36T, that will become operational in the summer of 2016 at the NHMFL will provide a view into the future for NMR spectroscopy; a future in which High Temperature Superconducting (HTS) magnets will have the potential for doubling the field strengths of current Low-Temperature superconducting (LTS) magnets. The SCH magnet is a hybrid magnet having an outer superconducting coil of 13T and inner stacks of Bitter plates that will be powered by 14MW and cooled by deionized water flowing through the stacks at 1700 gal/min. The SCH magnet will be the highest homogeneity and the highest stability resistive magnet that has been constructed, but the homogeneity and stability will not be the same as that for LTS magnets. The BTRR will develop a biomedical research focus on this magnet through the development of additional technology using a cross section of scientists committed to developing and pushing the scientific frontiers of NMR spectroscopy at a field strength that is more than 50% higher than any other NMR spectrometer in the world. More than a decade of effort has now gone into developing new technologies to enhance the stability and homogeneity of this magnet. Two approaches are being taken: first, an advanced lock unit has been developed by Bruker and tested at the NHMFL and secondly, Prof. Jeffrey Schiano from Penn State has developed a cascade field regulation technology that takes advantage of an inductive pickup coil as well as NMR measurements to provide a feedback field correction. A new 1H detection HXY 1.3 mm MAS probe will be developed for structural biology solid state NMR. 1H detection has the potential to revolutionize solid state NMR in much the same way 1H detection revolutionized solution NMR 30 years ago. This revolution will open will facilitate the structural characterization of membrane proteins in lipid bilayers and potentially in native cellular membrane. This technology will be coupled with oriented samples ssNMR that provides complementary structural restraints. The signal to noise per unit of spectrometer time for spin ½ nuclei improves with approximately B03, but for quadrupolar nuclei the rate of enhancement can be even greater due to a wide variety of factors including relaxation effects. While there are many odd halves quadrupolar nuclei in biological systems the most common and important one is 17O. Oxygen atoms are the primary sites in biological macromolecules of catalytic and functional chemistry. A goal in this effort is to work toward developing 17O spectroscopy as a routine spectroscopic tool.

TR＆D3-项目摘要/摘要 现场强度的显着提高始终导致科学领域的新边界 通过NMR光谱法实现。通常，挑战和被视为的弊端 高场证明了他们最大的优势。系列连接的混合（SCH）磁铁，在 一个36T的领域，该领域将于2016年夏季在NHMFL上运作 NMR光谱的未来；高温超导（HTS）磁铁将具有的未来 电流低温超导（LTS）磁体的磁场强度加倍的潜力。 SCH磁铁是一种混合磁铁 这将由14MW供电，并通过以1700加仑/分钟流过堆栈的去离子水冷却。这 SCH磁铁将是最高的均匀性，并且是稳定性的最高抗磁铁 构造，但是同质性和稳定性与LTS磁铁的稳定性不同。 BTRR会 通过使用A开发其他技术，开发生物医学研究的重点 科学家的横截面致力于开发和推动NMR光谱的科学前沿 比世界上任何其他NMR光谱仪高出50％以上的场强。 十多年来的努力现在已经开发了新技术，以增强稳定性和 该磁铁的同质性。正在采用两种方法：首先，已经开发了一个高级锁单元 由布鲁克（Bruker）在NHMFL进行了测试，其次是宾夕法尼亚州的Jeffrey Schiano教授开发了 利用归纳拾音器线圈和NMR的级联场调节技术 测量以提供反馈场校正。新的1小时检测HXY HXY 1.3 mm MAS探测器将是 为结构生物学固态NMR开发。 1小时检测有可能彻底改变固态 NMR以几乎相同的1小时检测方式彻底改变了解决方案NMR 30年前。这场革命将开放 将促进脂质双层中膜蛋白的结构表征，并有可能在天然中 细胞膜。该技术将与提供的样品SSNMR相结合 完全结构的约束。 自旋½核每单位光谱仪时间的噪声信号通过大约B03改善，但 对于四极核，由于多种因素，包括 放松效果。虽然有许多奇数半极核系统是最常见的 重要的是17o。氧原子是催化和生物大分子的主要部位 功能化学。这项努力的目标是致力于开发17o光谱作为常规 光谱工具。