Understanding the mechanism of genetic transformation

了解遗传转化的机制

• 批准号: 10205077
• 负责人: DAVID A DUBNAU
• 金额: $ 64.17万
• 依托单位: RBHS-NEW JERSEY MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-06-01 至 2023-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10205077
• 关键词: ATP Hydrolysis; ATP phosphohydrolase; Address; Antibiotic Resistance; Architecture; Bacillus subtilis; Bacteria; Binding; Binding Proteins; Biochemistry; Cell Wall; Cell membrane; Cell surface; Cells; Cellular biology; Collaborations; Competence; Complex; Coupled; Cryoelectron Microscopy; Cytoplasm; Cytoplasmic Protein; DNA; DNA Binding; DNA-Binding Proteins; Energy-Generating Resources; Evolution; Genes; Genetic; Genetic Transcription; Genetic Transformation; Goals; Gram-Negative Bacteria; Growth; Horizontal Gene Transfer; Immune Evasion; Laboratories; Mediating; Membrane; Methodology; Microscopy; Modeling; Molecular; Molecular Biology; Molecular Machines; Physiology; Pilum; Play; Process; Protein Biochemistry; Proteins; Proton-Motive Force; Role; Source; Stress; Structural Protein; Structure; System; Testing; Transmembrane Transport; Ursidae Family; Virulence; X-Ray Crystallography; expectation; experience; experimental study; helicase; insight; mutant; nuclease; optical traps; periplasm; protein complex; protein transport; single molecule; structural biology; tool; translocase; uptake

Abstract Natural transformation in bacteria is an important mode of horizontal gene transfer. Generally, it contributes to bacterial evolution and specifically to the spread of antibiotic resistance and virulence genes. This proposal will use Bacillus subtilis as a model to address the fundamental, largely conserved mechanisms that enable the uptake of environmental DNA. It will address the binding of DNA to the surface of the cell, traversal through the cell wall and translocation of DNA across the cell membrane. This plan will provide broad insights into molecular details of transformation and will bring us closer to the long-term goal of understanding the mechanism of DNA uptake. It is proposed to investigate the energy sources for DNA uptake, the proteins involved, some of their structures and their interactions as components of a molecular machine. These aims will be pursued using the tools of cell biology, genetics, biochemistry and structural biology.

抽象的 细菌中的自然转化是水平基因转移的重要模式。 通常，它有助于细菌进化，特别有助于抗生素的扩散 抗性和毒力基因。该建议将使用枯草芽孢杆菌作为模型 解决了能够吸收的基本，很大程度上保守的机制 环境DNA。它将解决DNA与细胞表面的结合，遍历遍历 通过细胞壁和DNA跨细胞膜的易位。这个计划将 提供有关转化分子细节的广泛见解，将使我们更接近 理解DNA摄取机制的长期目标。提议 研究DNA摄取的能源，涉及的蛋白质，其中一些 结构及其相互作用作为分子机的组成部分。这些目标会 使用细胞生物学，遗传学，生物化学和结构生物学的工具来追求。