Center for Neural Circuits in Addiction

成瘾神经回路中心

• 批准号: 10200729
• 负责人: Mark John Thomas
• 金额: $ 194.41万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10200729
• 关键词: Address; Adoption; Anatomy; Animals; Behavior; Biological; Biomedical Technology; Brain; Brain Diseases; Brain region; Catalysis; Chronic; Cognition; Collaborations; Communities; Complement; Consumption; Custom; Data; Data Analyses; Data Collection; Development; Discipline; Drug Addiction; Economics; Exhibits; Exposure to; Fiber; Fluorescence; Functional disorder; Funding; Future; Goals; Head; Health; Image; Individual; Informatics; Information Dissemination; Institutes; Institution; Interruption; Knowledge; Label; Leadership; Maps; Measures; Microscope; Mind; Minnesota; Mission; Modality; Molecular; Monitor; National Institute of Drug Abuse; Neurons; Neurosciences; Neurosciences Research; Pattern; Pharmaceutical Preparations; Photometry; Pilot Projects; Positioning Attribute; Prevention; Process; Reagent; Relapse; Research; Research Personnel; Research Support; Resources; Serotyping; Signal Transduction; Specific qualifier value; Speed; Structure; Substance Use Disorder; Techniques; Technology; Testing; Training and Education; United States National Institutes of Health; Universities; Viral; Virus; Work; addiction; base; biomarker identification; cellular imaging; computational platform; connectome; cost; craving; design; effective therapy; imaging modality; innovation; innovative technologies; interest; mental function; multimodality; neural circuit; new technology; novel; novel therapeutics; optical imaging; promoter; relating to nervous system; tool; vector

PROJECT SUMMARY: Overall Addiction is a chronic relapsing brain disorder resulting from perturbations in neural circuits. Delineating these circuit perturbations should provide a host of opportunities to develop new therapies for addiction prevention and treatment. New technologies in neuroscience are revolutionizing our ability to measure and intervene in specified neural circuits. To take advantage, these technologies should be broadly distributed. We propose to create a NIDA Center for Neural Circuits in Addiction at the University of Minnesota (UMN) to further develop and disseminate these new techniques to produce groundbreaking work in addiction neuroscience. Based on our collective expertise, our strong base of collaborative addiction research and the support provided by our institution, our group at the UMN is in an excellent position to form this Center. It would comprise four new Research Cores: 1) The Viral Innovation Core (VIC) will assist investigators in applying state-of-the-art viral manipulation approaches to their studies of the anatomical, molecular and neural circuit bases of addiction. This Core will provide expertise for design of custom vectors, including guidance on combinations of AAV serotype, promoters, and fluorescent tags; 2) The Structural Circuits Core (SCC) will offer state-of-the-art anatomical mapping of neural circuits involved in addiction. Integrated with the University Imaging Center and UMN Informatics Institute, SCC will provide automated use of brain clearing technology paired with meso- and micro- scale imaging of the CNS; 3) The Imaging Cells during Behavior Core (ICBC) will offer a range of imaging modalities to monitor brain activity in behaving animals across a range of spatial and temporal scales. These modalities include fiber photometry, head-mounted miniature microscopes (“miniscopes”) and novel wide field- of-view optical imaging during behavior at both the mesoscopic and cellular levels. 4) The Addiction Connectome Core (ACC) will create a computational platform to integrate multimodal functional and structural data to test relationships between exposure to addictive drugs and neural connectivity. Availability of this platform should enable outside scholars from anywhere in the world to delineate drug-modified connectivity patterns and addiction-relevant biological variables, facilitating the identification of biomarkers for mental function and dysfunction. Our Center would provide to the research community: a) Education and training in new technologies; b) Access to tools, reagents and expertise for data collection and analysis; c) Further development and adoption of new technologies; d) Catalysis of new collaborations among users; and e) Dissemination of resulting research and new technologies to the wider addiction research community. The Pilot Project Core will facilitate use of the Cores for innovative pilot studies and push the envelope in neural circuit research. Under the Center Director’s leadership, the Administrative Core, with a panel of expert scientific advisors, would coordinate and support the efforts of the individual Cores. Our goal is for the Center to be a national resource for neural circuit research technologies that fuels high-impact, collaborative research to address critical knowledge gaps in our field.

项目概要：总体 成瘾是一种由神经回路紊乱引起的慢性复发性脑部疾病。 电路扰动应该提供大量机会来开发新的疗法来预防成瘾和 神经科学的新技术正在彻底改变我们测量和干预特定疾病的能力。 为了利用这些技术，我们建议创建一个神经回路。 NIDA 明尼苏达大学 (UMN) 成瘾神经回路中心进一步开发和 传播这些新技术，以在成瘾神经科学领域产生突破性的工作。 集体的专业知识，我们强大的协作成瘾研究基础以及我们的支持 机构，我们 UMN 的团队处于组建该中心的绝佳位置，它将由四个新的成员组成。 研究核心：1）病毒创新核心（VIC）将协助研究人员应用最先进的病毒 他们对成瘾的解剖学、分子和神经回路基础的研究采用了操纵方法。 Core 将提供定制载体设计的专业知识，包括 AAV 血清型组合的指导， 启动子和荧光标签；2) 结构电路核心 (SCC) 将提供最先进的解剖学 与大学成像中心和 UMN 整合的神经回路图谱。 SCC 信息学研究所将提供与中观和微观相结合的大脑清理技术的自动化使用 中枢神经系统的规模成像；3) 行为核心 (ICBC) 期间的成像细胞将提供一系列成像 在一系列空间和时间尺度上监测行为动物的大脑活动的方式。 模式包括光纤光度测定、头戴式微型显微镜（“微型显微镜”）和新颖的宽视场 介观和细胞水平行为过程中的视野外光学成像 4) 成瘾。 Connectome Core（ACC）将创建一个计算平台来集成多模式功能和结构 数据来测试成瘾药物暴露与神经连接之间的关系。 应该使来自世界任何地方的外部学者能够描绘药物修饰的连接模式， 与成瘾相关的生物变量，有助于识别心理功能的生物标志物和 我们的中心将为研究界提供： a) 新技术的教育和培训； b) 获得数据收集和分析的工具、试剂和专业知识 c) 进一步开发和采用； 新技术的推广； d) 促进用户之间的新合作；以及 e) 成果研究的传播； 试点项目核心将促进更广泛的成瘾研究社区的新技术的使用。 在中心主任的领导下，创新试点研究的核心和突破神经回路研究的极限。 在领导层、行政核心以及专家科学顾问小组的领导下，将协调和支持 我们的努力目标是使该中心成为神经回路研究的国家资源。 推动高影响力的协作研究的技术，以解决我们领域的关键知识差距。