Center for Neural Circuits in Addiction

成瘾神经回路中心

• 批准号: 10200729
• 负责人: Mark John Thomas
• 金额: $ 194.41万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10200729
• 关键词: Address; Adoption; Anatomy; Animals; Behavior; Biological; Biomedical Technology; Brain; Brain Diseases; Brain region; Catalysis; Chronic; Cognition; Collaborations; Communities; Complement; Consumption; Custom; Data; Data Analyses; Data Collection; Development; Discipline; Drug Addiction; Economics; Exhibits; Exposure to; Fiber; Fluorescence; Functional disorder; Funding; Future; Goals; Head; Health; Image; Individual; Informatics; Information Dissemination; Institutes; Institution; Interruption; Knowledge; Label; Leadership; Maps; Measures; Microscope; Mind; Minnesota; Mission; Modality; Molecular; Monitor; National Institute of Drug Abuse; Neurons; Neurosciences; Neurosciences Research; Pattern; Pharmaceutical Preparations; Photometry; Pilot Projects; Positioning Attribute; Prevention; Process; Reagent; Relapse; Research; Research Personnel; Research Support; Resources; Serotyping; Signal Transduction; Specific qualifier value; Speed; Structure; Substance Use Disorder; Techniques; Technology; Testing; Training and Education; United States National Institutes of Health; Universities; Viral; Virus; Work; addiction; base; biomarker identification; cellular imaging; computational platform; connectome; cost; craving; design; effective therapy; imaging modality; innovation; innovative technologies; interest; mental function; multimodality; neural circuit; new technology; novel; novel therapeutics; optical imaging; promoter; relating to nervous system; tool; vector

PROJECT SUMMARY: Overall Addiction is a chronic relapsing brain disorder resulting from perturbations in neural circuits. Delineating these circuit perturbations should provide a host of opportunities to develop new therapies for addiction prevention and treatment. New technologies in neuroscience are revolutionizing our ability to measure and intervene in specified neural circuits. To take advantage, these technologies should be broadly distributed. We propose to create a NIDA Center for Neural Circuits in Addiction at the University of Minnesota (UMN) to further develop and disseminate these new techniques to produce groundbreaking work in addiction neuroscience. Based on our collective expertise, our strong base of collaborative addiction research and the support provided by our institution, our group at the UMN is in an excellent position to form this Center. It would comprise four new Research Cores: 1) The Viral Innovation Core (VIC) will assist investigators in applying state-of-the-art viral manipulation approaches to their studies of the anatomical, molecular and neural circuit bases of addiction. This Core will provide expertise for design of custom vectors, including guidance on combinations of AAV serotype, promoters, and fluorescent tags; 2) The Structural Circuits Core (SCC) will offer state-of-the-art anatomical mapping of neural circuits involved in addiction. Integrated with the University Imaging Center and UMN Informatics Institute, SCC will provide automated use of brain clearing technology paired with meso- and micro- scale imaging of the CNS; 3) The Imaging Cells during Behavior Core (ICBC) will offer a range of imaging modalities to monitor brain activity in behaving animals across a range of spatial and temporal scales. These modalities include fiber photometry, head-mounted miniature microscopes (“miniscopes”) and novel wide field- of-view optical imaging during behavior at both the mesoscopic and cellular levels. 4) The Addiction Connectome Core (ACC) will create a computational platform to integrate multimodal functional and structural data to test relationships between exposure to addictive drugs and neural connectivity. Availability of this platform should enable outside scholars from anywhere in the world to delineate drug-modified connectivity patterns and addiction-relevant biological variables, facilitating the identification of biomarkers for mental function and dysfunction. Our Center would provide to the research community: a) Education and training in new technologies; b) Access to tools, reagents and expertise for data collection and analysis; c) Further development and adoption of new technologies; d) Catalysis of new collaborations among users; and e) Dissemination of resulting research and new technologies to the wider addiction research community. The Pilot Project Core will facilitate use of the Cores for innovative pilot studies and push the envelope in neural circuit research. Under the Center Director’s leadership, the Administrative Core, with a panel of expert scientific advisors, would coordinate and support the efforts of the individual Cores. Our goal is for the Center to be a national resource for neural circuit research technologies that fuels high-impact, collaborative research to address critical knowledge gaps in our field.

项目摘要：总体 成瘾是由神经回路的扰动引起的慢性脑疾病。描绘这些 电路扰动应为开发预防成瘾的新疗法提供很多机会 治疗。神经科学的新技术正在彻底改变我们测量和干预特定的能力 神经回路。为了利用优势，这些技术应大致分布。我们建议创建一个 明尼苏达大学（UMN）的NIDA神经循环中心成瘾中心，以进一步发展和 传播这些新技术，以在成瘾神经科学中产生突破性的工作。基于我们 集体专业知识，我们的协作成瘾研究的强大基础和我们的支持 机构，我们在UMN的小组在构成该中心的良好位置。它将包括四个新的 研究核心：1）病毒创新核心（VIC）将帮助调查人员应用最先进的病毒 操纵方法是他们研究成瘾的解剖学，分子和神经回路基础的方法。这 Core将为设计自定义向量的设计提供专业知识，包括有关AAV血清型组合的指南， 启动子和荧光标签； 2）结构电路核心（SCC）将提供最新的解剖学 涉及成瘾的神经回路的映射。与大学成像中心和UMN集成 SCC Informatics Institute，将提供对大脑清除技术的自动使用，并配对中和微型 中枢神经系统的比例成像； 3）行为核心（ICBC）期间的成像单元将提供一系列成像 在一系列空间和临时尺度上监测行为动物的大脑活动的方式。这些 模式包括光纤光度法，头部安装的微型显微镜（“ Miniscopes”）和新型的广阔田间 - 在介质和细胞水平上行为期间的视觉光学成像。 4）成瘾 Connectome Core（ACC）将创建一个计算平台，以集成多模式功能和结构 数据以测试接触添加剂药物与神经连通性之间的关系。该平台的可用性 应该使来自世界任何地方的外部学者能够描述毒品改造的连接模式和 与成瘾有关的生物学变量，支持生物标志物的心理功能和 功能障碍。我们的中心将为研究社区提供：a）新技术的教育和培训； b）获得数据收集和分析的工具，试剂和专业知识； c）进一步发展和采用 新技术； d）用户之间新合作的催化； e）传播由此产生的研究 以及更广泛的成瘾研究界的新技术。飞行员项目核心将有助于使用 创新试点研究的核心，并推动神经回路研究中的包膜。在中心主任的领导下 领导力，行政核心，由专家科学顾问组成，将协调和支持 各个核心的努力。我们的目标是使中心成为神经巡回赛研究的国家资源 助长高影响力，协作研究的技术，以解决我们领域的关键知识差距。