Cytogenetics Shared Resource

细胞遗传学共享资源

• 批准号: 10362637
• 负责人: Patricia Greipp
• 金额: $ 11.36万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-25 至 2024-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10362637
• 关键词: Adherence; Animal Model; Cell Culture Techniques; Chromosomes; Classification; Clinic; Clinical; Communities; Comprehensive Cancer Center; Computer software; Consult; Consultations; Cost Control; Custom; Cytogenetic Analysis; Cytogenetics; Cytogenetics Shared Resource; DNA; DNA Probes; Data; Development; Environment; Equipment; Experimental Designs; Fluorescent in Situ Hybridization; Formalin; Funding; Grant; Individual; Institutes; Laboratories; Laboratory Research; Lead; Location; Loss of Heterozygosity; Mayo Clinic Cancer Center; Microscope; Modification; Molecular Cytogenetics; Paraffin Embedding; Peer Review; Production; Protocols documentation; Publications; RNA; Research; Research Personnel; Resource Sharing; Sampling; Services; Signal Transduction; Specimen; Technical Expertise; Techniques; Testing; Time; TimeLine; Training; Translations; biomarker discovery; cost; cost effective; diagnostic assay; experience; experimental study; human model; large datasets; member; novel; programs; research study; tool; tumor; whole genome

CYTOGENETICS SHARED RESOURCE PROJECT SUMMARY The central focus of the Cytogenetics Shared Resource (CYT) is to provide high quality cytogenetic and molecular cytogenetic analysis of human and animal model research samples in a timely, cost-effective manner for members of the Mayo Clinic Cancer Center (MCCC) and other investigators within the Mayo research community. External requests are accommodated with discretion. The highly specialized cytogenetic and molecular cytogenetic services and equipment utilized to provide such services to MCCC users would be impractical to institute into multiple individual laboratories. These services are ideally provided in a central location and in a Shared Resource environment, which eliminates the need to duplicate expensive equipment and technologist training and analysis time. Services within the CYT include: Cell Culture, Routine Chromosome Analysis, DNA and RNA fluorescence in situ Hybridization (FISH), and Custom DNA Probe Production as well as RNA FISH Probe Production and a Tyramide Signal Amplification (TSA) protocol. Together, with the investigator, the CYT assists in determining how these techniques can be utilized to meet the evolving needs of their research studies. In addition to the test menu described above, the CYT staff also provides specialized training to investigators for FISH set-up, and microscope use in an effort to minimize investigator cost and economize technologist time. The staff that makes up the CYT have over 75 years of combined cytogenetic and molecular cytogenetic expertise. The capability provided by the CYT is not present in any other Shared Resource facility or research laboratory at Mayo Clinic. The 6 highly qualified and experienced staff members (3.6 FTE) provide expert consultation in experimental design, troubleshooting, modification of experiments, and interpretation of results. The staff also serves to help facilitate the translation of molecular cytogenetic discoveries into clinically available diagnostic assays. In the past grant cycle (2013- 2017), the CYT had a total of 208 users, representing 10 of the 10 MCCC Programs active at the time. Among our 208 users, 84 (40.4%) are MCCC members. Of these 84 investigators, 57 (68%) have active peer- reviewed funding. During these past 5 years, the CYT supported the production of 36 peer-reviewed publications.

细胞遗传学共享资源项目摘要 细胞遗传学共享资源（CYT）的主要重点是提供高质量的细胞遗传学和 人类和动物模型研究样本的分子细胞遗传学分析及时，成本效益 Mayo诊所癌症中心（MCCC）和Mayo中其他研究人员的方式 研究社区。外部请求可容纳自由裁量权。高度专业的细胞遗传学 以及用于向MCCC用户提供此类服务的分子细胞遗传学服务和设备 不切实际地建立多个单个实验室。这些服务是理想的中央提供的 位置和共享资源环境，这消除了复制昂贵设备的需求 以及技术专家培训和分析时间。 Cyt中的服务包括：细胞培养，常规 染色体分析，DNA和RNA荧光原位杂交（FISH）和自定义DNA探针 生产以及RNA鱼探针产生和泰米酰胺信号扩增（TSA）方案。 与研究者一起，CYT有助于确定如何使用这些技术来满足 他们的研究的不断发展的需求。除上述测试菜单外，Cyt员工还 向调查人员提供专门的培训以进行鱼类设置，并使用显微镜使用以最大程度地减少 研究人员的成本和节能技术专家时间。组成Cyt的工作人员有75年以上 结合细胞遗传学和分子细胞遗传学专业知识。 Cyt提供的功能不存在 在梅奥诊所的任何其他共享资源设施或研究实验室中。 6个高度合格的 经验丰富的工作人员（3.6 FTE）提供有关实验设计，故障排除的专家咨询， 修改实验和结果解释。工作人员还有助于促进翻译 分子细胞遗传学发现成临床可用的诊断测定。在过去的赠款周期（2013-- 2017年），CYT共有208位用户，代表当时活跃的10个MCCC计划中的10个。之中 我们的208个用户，84（40.4％）是MCCC成员。在这84位研究者中，有57位（68％）具有活跃的同行 审查资金。在过去的5年中，Cyt支持了36个同行评审的生产 出版物。