Maintaining and Expanding the CHAMACOS Epidemiology Cohort Infrastructure for Future Generations

为子孙后代维护和扩展 CHAMACOS 流行病学队列基础设施

• 批准号: 10200041
• 负责人: Brenda Eskenazi
• 金额: $ 29.05万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-30 至 2022-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10200041
• 关键词: 1 year old; 14 year old; 21 year old; Adolescence; Adult; Affect; Aging; Agriculture; Allergens; Arylesterase; Back; Big Data; Biological; Biological Markers; Biological Specimen Banks; Biological Specimen database; Birth; Blood; California; Chemicals; Child; Cohort Studies; Collaborations; Communication; Communities; DNA Methylation; Data; Data Management Resources; Database Management Systems; Databases; Development; Documentation; Dust; Ensure; Environmental Epidemiology; Environmental Exposure; Environmental Impact; Epidemiology; Exposure to; Family; Feasibility Studies; Flame Retardants; Freezing; Funding; Future; Future Generations; Genomics; Grant; Growth; Hair; Health; Human Milk; Infrastructure; Institution; International; Isoprostanes; Knowledge; Latino; Life; Life Cycle Stages; Low income; Lung diseases; Maintenance; Methodology; Methods; MicroRNAs; Modeling; Molecular; Neighborhoods; Newsletter; Obesity; Outcome; Paper; Participant; Pesticides; Pilot Projects; Population; Protocols documentation; Publications; Publishing; Reporting; Research; Research Personnel; Research Project Grants; Resources; Running; Safety; Salinas Valley; Saliva; Sampling; Scientific Advances and Accomplishments; Specimen; Standardization; System; Time; United States National Institutes of Health; Urine; Visit; Youth; adipokines; agricultural community; archive data; archived data; biobank; cohort; community based participatory research; community engagement; data management; data portal; data sharing; deciduous tooth; early life exposure; early pregnancy; farm worker; improved; laboratory facility; metabolomics; microbiome; neurodevelopment; novel; participant retention; postnatal; preservation; pulmonary function; repaired; validation studies; young adult

ABSTRACT Since its inception in 1999, the CHAMACOS study is one of the longest running cohort studies examining the impact of early life environmental exposures on neurodevelopment, growth, and respiratory disease and the only one focused on low-income, Latino children in a farmworker population. We have collected extensive health, exposure, demographic, neighborhood, and regional data, as well as biological (e.g. blood, urine, breastmilk, hair, saliva, deciduous teeth) and environmental (e.g. dust, allergens) samples at multiple visits and have created a large biorepository with more than 220,000 samples stored for future use. With over 140 publications, CHAMACOS is a successful and well-established environmental epidemiology cohort. We have used banked specimens and archived data to demonstrate relationships of pre- and postnatal exposures to pesticides, flame retardants, and other chemicals with poorer neurodevelopment, reduced lung function, obesity, and other outcomes. We have shown that environmental exposures affect a multitude of molecular mechanisms that influence health, such as PON1 enzymatic activity, adipokine and isoprostane levels, DNA methylation and miRNA expression. The CHAMACOS resources have supported multiple NIH, EPA, and non-federal grants and trainees, including collaborations with other institutions. However, the infrastructure required for management of this vast trove of data, the laboratory facilities to ensure the safety of hundreds of thousands of samples, and the effort to keep families engaged and participating in this long- running study have increased over time, while funding for these activities has decreased. The aims for this proposal are to (1) retain participation in the cohort through community engagement, (2) maintain and strengthen data management infrastructure, (3) maintain and enhance the existing biorepository through replacement, repair, and maintenance of aging deep freezers to ensure the integrity of samples and improvements to systems to track samples used for multiple research grants, pilot studies, and collaborations, (4) conduct validation, pilot, and feasibility studies to investigate new methods of assessing environmental exposures, develop best practices for biorepositories, and explore novel methodologies related to metabolomics, genomics, and the microbiome, and (5) develop a data sharing portal to encourage use by outside collaborators of existing CHAMACOS data. In summary, infrastructure support for the CHAMACOS cohort study will preserve specimens, ensure well- documented data for future studies and data sharing, and maintain participant retention, assuring effective future use of these valuable resources. This maintenance grant will thereby strengthen our ability to answer key questions about the impact of environmental exposures on health over the life course and will assure that the extensive resources generated by the CHAMACOS study will be effectively used by investigators worldwide for years to come.

抽象的 自 1999 年启动以来，CHAMACOS 研究是运行时间最长的队列研究之一 生命早期环境暴露对神经发育、生长和呼吸系统疾病的影响 唯一一个关注农场工人中低收入拉丁裔儿童的项目。我们收集了大量 健康、暴露、人口、邻里和区域数据，以及生物数据（例如血液、尿液、 多次访视时采集母乳、头发、唾液、乳牙）和环境（例如灰尘、过敏原）样本 创建了一个大型生物样本库，存储了超过 220,000 个样本以供将来使用。拥有超过140 CHAMACOS 是一个成功且完善的环境流行病学队列。 我们使用储存的标本和存档数据来证明产前和产后的关系 接触农药、阻燃剂和其他化学物质会导致神经发育较差、肺功能下降 功能、肥胖和其他结果。我们已经证明，环境暴露会影响许多方面 影响健康的分子机制，例如 PON1 酶活性、脂肪因子和异前列烷 水平、DNA 甲基化和 miRNA 表达。 CHAMACOS 资源已支持多个 NIH、 EPA 以及非联邦拨款和培训生，包括与其他机构的合作。然而， 管理这一海量数据所需的基础设施、确保数据安全的实验室设施 数十万个样本，以及让家庭参与这一长期活动的努力 随着时间的推移，运行研究的数量不断增加，而对这些活动的资助却在减少。 该提案的目的是（1）通过社区参与保持对队列的参与，（2） 维护和加强数据管理基础设施，（3）维护和增强现有的生物样本库 通过更换、维修和保养老化的深冷柜，确保样品和样品的完整性 改进用于跟踪用于多项研究资助、试点研究和合作的样本的系统， （四）开展验证、试点和可行性研究，探索环境评价新方法 暴露，开发生物样本库的最佳实践，并探索与 代谢组学、基因组学和微生物组，以及 (5) 开发一个数据共享门户以鼓励使用 现有 CHAMACOS 数据的外部合作者。 总之，对 CHAMACOS 队列研究的基础设施支持将保存标本，确保 记录数据以供未来研究和数据共享，并保留参与者，确保有效 未来如何利用这些宝贵的资源。因此，这笔维持补助金将增强我们回答问题的能力 关于生命周期中环境暴露对健康影响的关键问题，并将确保 CHAMACOS 研究产生的广泛资源将被研究人员有效利用 未来几年将在全球范围内。