Structural Biology (SB)

结构生物学（SB）

• 批准号: 9981653
• 负责人: Gloria Borgstahl
• 金额: $ 11.44万
• 依托单位: UNIVERSITY OF NEBRASKA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-05 至 2021-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9981653
• 关键词: Active Sites; Applications Grants; Biological; Businesses; Cancer Center; Cancer Center Support Grant; Crystallization; Data; Data Collection; Disease; Fees; Funding; Goals; Growth; Knowledge; Laboratories; Malignant Neoplasms; Nebraska; Nuclear Magnetic Resonance; Peer Review; Production; Publications; Research; Roentgen Rays; Running; Sampling; Solid; Structure; Techniques; United States National Aeronautics and Space Administration; United States National Institutes of Health; Visualization; X-Ray Crystallography; anticancer research; biophysical techniques; cost; instrumentation; macromolecule; member; novel therapeutics; protein expression; protein protein interaction; protein purification; screening; structural biology; structured data; three dimensional structure; Active Sites; Applications Grants; Biological; Businesses; Cancer Center; Cancer Center Support Grant; Crystallization; Data; Data Collection; Disease; Fees; Funding; Goals; Growth; Knowledge; Laboratories; Malignant Neoplasms; Nebraska; Nuclear Magnetic Resonance; Peer Review; Production; Publications; Research; Roentgen Rays; Running; Sampling; Solid; Structure; Techniques; United States National Aeronautics and Space Administration; United States National Institutes of Health; Visualization; X-Ray Crystallography; anticancer research; biophysical techniques; cost; instrumentation; macromolecule; member; novel therapeutics; protein expression; protein protein interaction; protein purification; screening; structural biology; structured data; three dimensional structure

Project Summary/Abstract: Structural Biology Facility The overall goal of the Structural Biology (SB) Facility is to apply three-dimensional structural visualization techniques to the analysis of important cancer-related biological macromolecules. Such structural data can provide basic knowledge of disease mechanism and drive research. Atomic visualization of active sites and protein-protein interactions can direct the synthesis of novel therapeutics. The co-directors and staff of the SB Facility make access to these sophisticated, highly-technical biophysical methods easy for members of the cancer center. The SB Facility was initiated ten years ago, through funding from the Nebraska Research Initiative (NRI) and the Vice Chancellor for Research. The SB Facility has five complimentary laboratory components: Protein expression and purification (PrEP), crystal screening and growth (CSG), X-ray crystallography data collection, small-angle X-ray scattering (SAXS) data collection and nuclear magnetic resonance (NMR) data collection. The PrEP component is a central part of the facility and prepares samples for CSG (and subsequently X-ray crystallography), SAXS and/or NMR. The SB Facility is run with a solid “collaborative” business plan that includes fees collected upon production of samples and/or use of the instrumentation, as well as, inclusion of costs in collaborative grant proposals. Data provided from the SB Facility has helped fund numerous external grant proposals including those from NIH, NSF and NASA. Numerous collaborative, peer-reviewed publications in cancer research as well as invited review articles have resulted from the SB Facility.

项目摘要/摘要：结构生物学设施 结构生物学（SB）设施的总体目标是应用三维结构可视化 分析重要的癌症相关生物学大分子的技术。这样的结构数据可以 提供疾病机制的基本知识并推动研究。活性位点的原子可视化和 蛋白质 - 蛋白质相互作用可以指导新治疗的合成。 SB的联合导演和工作人员 设施使这些精致，高科技的生物物理方法易于使用 癌症中心。 SB设施是十年前通过内布拉斯加州研究的资助 倡议（NRI）和研究副校长。 SB设施有五个免费实验室 成分：蛋白质表达和纯化（PREP），晶体筛选和生长（CSG），X射线 晶体学数据收集，小角度X射线散射（SAXS）数据收集和核磁 共振（NMR）数据收集。准备组件是设施的中心部分，并准备样品 对于CSG（以及随后的X射线晶体学），SAXS和/或NMR。 SB设施以坚固的方式运行 “协作”业务计划，其中包括生产样品时收取的费用和/或使用 仪器以及将成本包括在协作赠款建议中。 SB提供的数据 设施帮助资助了包括NIH，NSF和NASA在内的众多外部赠款建议。 癌症研究和受邀评论文章中的许多合作，经过同行评审的出版物 由SB设施产生。