Analyzing pathogenic Vibrio parahaemolyticus-oyster interactions to prevent human disease

分析致病性副溶血性弧菌与牡蛎的相互作用以预防人类疾病

基本信息

批准号：
9979077
负责人：
Ann M Stevens
金额：
$ 8.01万
依托单位：
VIRGINIA POLYTECHNIC INST AND ST UNIV
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-03-01 至 2022-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9979077
关键词：
Acute Adolescent Animal Model Animals Aquaculture Bacteria Bacterial Proteins Bacteriology Beds Bioinformatics Biological Models Biology Cells Centers for Disease Control and Prevention (U.S.)Clinical Consumption Detection Development Disease Eating Ecology Environmental Risk Factor Epithelial Epithelium Excision Food Food Safety Foundations Future Gastroenteritis Genes Genetic Goals Harvest Health Human Immunocompromised Host Individual Infection Intervention Laboratories Lead Libraries Life Location Methods Mission Modeling Molecular Morbidity - disease rate Oysters Palliative Care Pathogenicity Pathology Phase Play Prevention strategy Production Public Health Regulation Research Role Route Scientist Seafood Shellfish Site Source System Systemic infection Testing Tissues United States United States National Institutes of Health Vibrio Vibrio parahaemolyticus Virulence Water Work animal breeding animal tissue base disorder prevention economic impact emerging human pathogen food surveillance foodborne illness gastrointestinal genetic approach genome-wide human disease human morbidity human mortality improved in vivo infection rate insight method development microbiome mortality mutant novel novel strategies open wound pandemic disease pathogen pathogenic bacteria prevent risk minimization therapy development transmission process transposon sequencing undercooked

Acute Adolescent Animal Model Animals Aquaculture Bacteria Bacterial Proteins Bacteriology Beds Bioinformatics Biological Models Biology Cells Centers for Disease Control and Prevention (U.S.)Clinical Consumption Detection Development Disease Eating Ecology Environmental Risk Factor Epithelial Epithelium Excision Food Food Safety Foundations Future Gastroenteritis Genes Genetic Goals Harvest Health Human Immunocompromised Host Individual Infection Intervention Laboratories Lead Libraries Life Location Methods Mission Modeling Molecular Morbidity - disease rate Oysters Palliative Care Pathogenicity Pathology Phase Play Prevention strategy Production Public Health Regulation Research Role Route Scientist Seafood Shellfish Site Source System Systemic infection Testing Tissues United States United States National Institutes of Health Vibrio Vibrio parahaemolyticus Virulence Water Work animal breeding animal tissue base disorder prevention economic impact emerging human pathogen food surveillance foodborne illness gastrointestinal genetic approach genome-wide human disease human morbidity human mortality improved in vivo infection rate insight method development microbiome mortality mutant novel novel strategies open wound pandemic disease pathogen pathogenic bacteria prevent risk minimization therapy development transmission process transposon sequencing undercooked

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项目摘要

Project Summary/Abstract The bacterium Vibrio parahaemolyticus (VP) is a leading cause of seafood-borne gastroenteritis on a pandemic scale. In the United States, it is a CDC-reportable emerging human pathogen with increasing annual morbidity and mortality rates. Diseases caused by pathogenic VP strains have important implications for global food safety and sustainability in relation to human health. Most commonly, human disease prevention has focused on the detection and reduction of VP in food sources. Despite this, human VP infection rates are still rising. Little is known about how VP interacts with its marine animal hosts. A better understanding of this relationship has the potential to lead to the development of methods to either prevent bacterial-animal host association and/or enhance bacterial removal from the host prior to human consumption, thereby mitigating the occurrence of human disease. We propose to use oysters, a natural host of VP and a common route of transmission to humans, as an animal model system to understand when and how VP interacts with them. It is hypothesized that A) VP will localize to specific tissues in the oyster and B) specific genes will be critical for the ability of VP to colonize oysters. The proposed work is strengthened by our collaborative research team, which is comprised of four scientists with expertise in molecular bacteriology, shellfish aquaculture, animal pathology and bioinformatics methods. The novel information gained from this research will lead to a more reliable laboratory-based oyster model system that will lay the foundation for future work to establish the roles environmental factors, including the resident microbiome, host genetics and specific bacterial gene products play in VP-oyster interactions. The refined oyster model will be applicable not only for VP but for other pathogenic Vibrio species, leading to improved methods to prevent human food-borne disease acquisition.

项目摘要/摘要细菌弧菌性溶血症（VP）是海鲜传播胃肠炎的主要原因大流行量表。在美国，它是一种可降低的人类病原体，年度增加发病率和死亡率。病原体VP菌株引起的疾病对全球具有重要意义食品安全与可持续性与人类健康有关。最常见的是，预防人类疾病专注于食物来源中VP的检测和减少。尽管如此，人类副总裁感染率仍然上升。关于VP如何与海洋动物宿主相互作用的知之甚少。对此有更好的理解关系有可能导致开发方法，以防止细菌 - 敌对宿主在人类消费之前，与宿主的关联和/或增强细菌去除，从而减轻人类疾病的发生。我们建议使用牡蛎，自然的副总裁和一条公共路线向人类传播，作为一种动物模型系统，以了解VP何时以及如何与它们相互作用。这是假设a）VP将定位于牡蛎中的特定组织，b）特定基因对副总裁殖民牡蛎的能力。我们的合作研究团队加强了拟议的工作，该团队由四名具有分子细菌学，贝类水产养殖，动物病理学专业知识的科学家组成和生物信息学方法。从这项研究中获得的新颖信息将导致更可靠的基于实验室的牡蛎模型系统，将为将来的工作奠定基础环境因素，包括常驻微生物组，宿主遗传学和特定细菌基因产物玩VP-oyster互动。精制牡蛎型不仅适用于副总裁，而且适用于其他致病性弧菌物种，导致改进的方法可预防人类饮食疾病的获取。