CRNS: An Integrative Study of Hippocampal-Neocortical Memory Coding during Sleep

CRNS：睡眠期间海马-新皮质记忆编码的综合研究

• 批准号: 9920779
• 负责人: Zhe Sage Chen
• 金额: $ 35.85万
• 依托单位: NEW YORK UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-12 至 2023-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9920779
• 关键词: Acoustic Stimulation; Address; Animals; Area; Auditory; Code; Computing Methodologies; Coupled; Cues; Data; Dependence; Dreams; Ego; Electrophysiology (science); Goals; Hippocampus (Brain); Human; Impairment; Learning; Light; Location; Memory; Mental disorders; Methods; Neocortex; Neurons; Phase; Population; Process; REM Sleep; Rattus; Rodent; Role; Sensory; Sleep; Sleep Stages; Subgroup; Task Performances; Techniques; Therapeutic; Ursidae Family; Vision; Visual; Visual Cortex; Visual system structure; Visuospatial; Wakefulness; Work; area striata; cell assembly; experience; experimental study; information processing; innovation; insight; memory consolidation; memory process; negative affect; neocortical; nervous system disorder; non rapid eye movement; novel; optogenetics; rapid eye movement; relating to nervous system; sensory input; spatial memory; visual imagery; visual memory; way finding

Sleep is critical to memory and learning. During rapid eye movement (REM) or non-REM (NREM) sleep, subgroups of cell assemblies in hippocampal and sensory cortical circuits are reactivated in a temporally coordinated manner, forming a cortical-hippocampal-cortical loop of information processing during memory consolidation. Deciphering neural codes of hippocampal-neocortical memories during sleep would reveal important circuit mechanisms of memory consolidation. To date, a complete understanding of the mechanisms of hippocampal-neocortical memory processing and the interaction of their specific spatial/non­ spatial memory representations during sleep is lacking. Furthermore, little is known about the causal impact of the hippocampal-neocortical interactions on subsequent memory reactivation or post-sleep learning. In this proposal, we will dissect representations of spatial ("where") and visual ("what") memory in the rodent hippocampal CA1 and primary visual cortex (V1) during sleep. We will combine electrophysiology, population-decoding methods, optogenetics and closed-loop neural interface to decipher sleep-associated CA1-V1 population codes in memory coding. In Aim 1, we will identify visual cortical representations in a spatial navigation task and determine visual cortical neuronal firing dependency on space, experiences and visual cues. In Aim 2, we will uncover "where" (spatial) and "what" (visual) representations of CA1-V1 memory reactivations during sleep. In Aim 3, we will determine the causal role of the hippocampus in the V1-CA1-V1 loop of memory consolidation during sleep. Together, these results will enable us to casually dissect circuit mechanisms of hippocampal-neocortical memory coding during sleep, and to establish a new analysis paradigm to identify the contents of hippocampal-memory reactivations during sleep. Our project will provide further insight into memory-related neurological and psychiatric disorders and potential therapeutic treatment for targeted memory reactivation or enhancement.

睡眠对于记忆和学习至关重要。在快速眼动 (REM) 或非快速眼动 (NREM) 睡眠期间，海马和感觉皮层回路中的细胞组件亚群以时间协调的方式重新激活，形成皮质-海马-皮质环路。破译睡眠期间海马新皮质记忆的神经编码将揭示记忆巩固的重要回路机制。此外，我们对海马-新皮质相互作用对随后的记忆重新激活或睡眠后学习的因果影响知之甚少。 ，我们将剖析啮齿类动物海马 CA1 和初级视觉皮层 (V1) 睡眠期间的空间（“哪里”）和视觉（“什么”）记忆的表征。群体解码方法、光遗传学和闭环神经接口来破译记忆编码中与睡眠相关的 CA1-V1 群体代码。在目标 1 中，我们将识别空间导航任务中的视觉皮层表征，并确定视觉皮层神经放电对空间的依赖性。在“目标 2”中，我们将揭示睡眠期间 CA1-V1 记忆重新激活的“何处”（空间）和“什么”（视觉）表征。 3、我们将确定海马在睡眠期间记忆巩固的V1-CA1-V1环路中的因果作用，这些结果将使我们能够随意剖析睡眠期间海马-新皮质记忆编码的回路机制，并建立一个模型。确定睡眠期间海马记忆重新激活内容的新分析范式将进一步深入了解与记忆相关的神经和精神疾病以及针对目标记忆重新激活或增强的潜在治疗方法。