ShEEP Request for BZX Fluorescence Microscope

ShEEP 请求 BZX 荧光显微镜

• 批准号: 9905078
• 负责人: Shijie Song
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9905078
• 关键词: 15 year old; 3-Dimensional; Affect; Alzheimer' s Disease; Biomedical Research; Clinical; Development; Diabetes Mellitus; Disease; Equipment; Florida; Fluorescence; Funding; Goals; Grant; Gulf War; Healthcare; Image; Individual; Investigation; Journals; Laboratories; Lighting; Malignant Neoplasms; Measurement; Medical center; Microscope; Mission; Noise; Obesity; Optics; Positioning Attribute; Productivity; Publications; Quality of Care; Regenerative Medicine; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Scanning; Scientist; Sheep; Signal Transduction; Slide; Specific qualifier value; Specimen; Structure; System; Thick; Time; Translational Research; Transportation; Traumatic Brain Injury; United States Department of Veterans Affairs; United States National Institutes of Health; Universities; Veterans; Veterans Hospitals; career; college; fluorescence microscope; instrument; instrumentation; novel; operation; preference; programs; respiratory; synergism; 15 year old; 3-Dimensional; Affect; Alzheimer' s Disease; Biomedical Research; Clinical; Development; Diabetes Mellitus; Disease; Equipment; Florida; Fluorescence; Funding; Goals; Grant; Gulf War; Healthcare; Image; Individual; Investigation; Journals; Laboratories; Lighting; Malignant Neoplasms; Measurement; Medical center; Microscope; Mission; Noise; Obesity; Optics; Positioning Attribute; Productivity; Publications; Quality of Care; Regenerative Medicine; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Scanning; Scientist; Sheep; Signal Transduction; Slide; Specific qualifier value; Specimen; Structure; System; Thick; Time; Translational Research; Transportation; Traumatic Brain Injury; United States Department of Veterans Affairs; United States National Institutes of Health; Universities; Veterans; Veterans Hospitals; career; college; fluorescence microscope; instrument; instrumentation; novel; operation; preference; programs; respiratory; synergism

The proposal requests KEYENCE BZ-X810 Fluorescence Microscope as a common resource shared major equipment integral to the core lab facility to be used by biomedical researchers of the James A Haley Veterans Hospital (JAHVH). Biomedical research at JAHVH is conducted by renowned, well-funded investigators. The proposed instrument will be predominantly used by 12 investigators, including 2 Senior Research Career Scientists and 2 Research Career Scientists with ongoing research programs related to Alzheimer's Disease, cancer, diabetes, obesity, Gulf War Illness, respiratory syncytial disease, traumatic brain injury and regenerative medicine. In addition, these individuals have other grants (NIH, AHA, FLDOH, DoD, etc.) that will utilize the fluorescence microscope to help build on existing and new research projects that will benefit Veterans. This acquisition will be a much-needed replacement for the greater than 15-year- old defunct microscope presently available for use. No other multi-functional fluorescence microscopes are available within JAHVH and the one available at the University of South Florida (USF, our affiliate university) is in high-demand within USF, shared by 5 colleges and often time-slots are unavailable as VA researchers are not given preference over USF researchers. Additionally, transportation to USF causes delays adversely affecting samples. This fluorescence microscope has a lot of benefits: Compact size saves benchtop space; Easy operation for all users; Sensitive optics deliver high-quality results; Specify measurement range; Automatically batch capture desired images; High-content analysis with uniform conditions; multi-dimensional image capture with-high-resolution-stitching and Z-stacking; Structured illumination eliminates fluorescence blurring and delivers clear images in just one click; Capture an entire specimen automatically by registering the coordinates of its outermost positions; Accurate analysis of structure and 3D signals. The multi-functional fluorescence microscope captures images at a higher resolution with high sensitivity compared to conventional microscope. Multi-function is built all-in-one, the dark room is not needed. It can scan multi-slides at the same time. Other benefits of this microscope include enhanced signal-to-noise ratio, comprehensible and close examination of thick samples, High quality images are the crux of research projects and are central to publications in high impact journals and obtaining federal funding. Moreover, the instrumentation will accelerate and enhance the research productivity of the JAHVH investigators, allow for development of new research directions, increase synergy between investigators, and increase the translational research capacity of the JAHVH Research Program. Overall, the proposed instrument will significantly impact and augment VAMC's mission to advance the healthcare of Veterans by allowing VA researchers to conduct cutting-edge research in basic, translational or clinical fields. These endeavors will substantially contribute to the long-term research goals of the JAHVH.

该提案要求Keyence BZ-X810荧光显微镜作为公共资源 共享核心实验室设施不可或缺的主要设备，将由生物医学研究人员使用 James A Haley退伍军人医院（JAHVH）。 JAHVH的生物医学研究由 著名，资金充足的调查员。所提出的仪器将主要使用12 研究人员，包括2位高级研究职业科学家和2名研究职业科学家 随着与阿尔茨海默氏病，癌症，糖尿病，肥胖，海湾有关的持续研究计划 战争疾病，呼吸道疾病，创伤性脑损伤和再生医学。在 此外，这些人还有其他赠款（NIH，AHA，FLDOH，DOD等） 荧光显微镜以帮助建立现有和新的研究项目，这将受益 退伍军人。 此次收购将是大于15岁以上已解散的急需的替代品 显微镜目前可用于使用。没有其他多功能荧光显微镜是 可在JAHVH内提供，并在南佛罗里达大学提供（USF，我们的 会员大学）在USF内的高需求，由5所大学共享，通常是时间表 由于VA研究人员没有比USF研究人员的偏好，因此无法获得。此外， 向USF的运输导致延迟对样本的不利影响。 该荧光显微镜具有很大的好处：紧凑尺寸可节省台式空间；简单的 所有用户的操作；敏感的光学元件可提供高质量的结果；指定测量范围； 自动批量捕获所需的图像；具有统一条件的高含量分析； 多维图像捕获具有高分辨率缝制和Z堆积的捕获；结构 照明消除了荧光模糊，仅一键单击即可提供清晰的图像。捕获 通过注册其最外部位置的坐标自动一个整个标本； 精确分析结构和3D信号。 多功能荧光显微镜以较高的分辨率捕获图像 与常规显微镜相比，灵敏度。多功能是多合一的，黑暗的房间 不需要。它可以同时扫描多扫描。该显微镜的其他好处 包括增强的信噪比，可理解和仔细检查厚样品， 高质量的图像是研究项目的症结所在，是出版物的核心 影响期刊并获得联邦资金。此外，仪器将加速并 提高JAHVH调查人员的研究生产率，允许开发新的 研究方向，增加研究人员之间的协同作用，并增加翻译 JAHVH研究计划的研究能力。 总体而言，拟议的工具将极大地影响和扩大VAMC的使命 通过允许VA研究人员进行尖端，推动退伍军人的医疗保健 基本，转化或临床领域的研究。这些努力将大大贡献 JAHVH的长期研究目标。