ShEEP Request for BZX Fluorescence Microscope

ShEEP 请求 BZX 荧光显微镜

• 批准号: 9905078
• 负责人: Shijie Song
• 金额: --
• 依托单位: JAMES A. HALEY VA MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2020-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9905078
• 关键词: 15 year old; 3-Dimensional; Affect; Alzheimer' s Disease; Biomedical Research; Clinical; Development; Diabetes Mellitus; Disease; Equipment; Florida; Fluorescence; Funding; Goals; Grant; Gulf War; Healthcare; Image; Individual; Investigation; Journals; Laboratories; Lighting; Malignant Neoplasms; Measurement; Medical center; Microscope; Mission; Noise; Obesity; Optics; Positioning Attribute; Productivity; Publications; Quality of Care; Regenerative Medicine; Request for Proposals; Research; Research Personnel; Research Project Grants; Resolution; Resource Sharing; Resources; Sampling; Scanning; Scientist; Sheep; Signal Transduction; Slide; Specific qualifier value; Specimen; Structure; System; Thick; Time; Translational Research; Transportation; Traumatic Brain Injury; United States Department of Veterans Affairs; United States National Institutes of Health; Universities; Veterans; Veterans Hospitals; career; college; fluorescence microscope; instrument; instrumentation; novel; operation; preference; programs; respiratory; synergism

The proposal requests KEYENCE BZ-X810 Fluorescence Microscope as a common resource shared major equipment integral to the core lab facility to be used by biomedical researchers of the James A Haley Veterans Hospital (JAHVH). Biomedical research at JAHVH is conducted by renowned, well-funded investigators. The proposed instrument will be predominantly used by 12 investigators, including 2 Senior Research Career Scientists and 2 Research Career Scientists with ongoing research programs related to Alzheimer's Disease, cancer, diabetes, obesity, Gulf War Illness, respiratory syncytial disease, traumatic brain injury and regenerative medicine. In addition, these individuals have other grants (NIH, AHA, FLDOH, DoD, etc.) that will utilize the fluorescence microscope to help build on existing and new research projects that will benefit Veterans. This acquisition will be a much-needed replacement for the greater than 15-year- old defunct microscope presently available for use. No other multi-functional fluorescence microscopes are available within JAHVH and the one available at the University of South Florida (USF, our affiliate university) is in high-demand within USF, shared by 5 colleges and often time-slots are unavailable as VA researchers are not given preference over USF researchers. Additionally, transportation to USF causes delays adversely affecting samples. This fluorescence microscope has a lot of benefits: Compact size saves benchtop space; Easy operation for all users; Sensitive optics deliver high-quality results; Specify measurement range; Automatically batch capture desired images; High-content analysis with uniform conditions; multi-dimensional image capture with-high-resolution-stitching and Z-stacking; Structured illumination eliminates fluorescence blurring and delivers clear images in just one click; Capture an entire specimen automatically by registering the coordinates of its outermost positions; Accurate analysis of structure and 3D signals. The multi-functional fluorescence microscope captures images at a higher resolution with high sensitivity compared to conventional microscope. Multi-function is built all-in-one, the dark room is not needed. It can scan multi-slides at the same time. Other benefits of this microscope include enhanced signal-to-noise ratio, comprehensible and close examination of thick samples, High quality images are the crux of research projects and are central to publications in high impact journals and obtaining federal funding. Moreover, the instrumentation will accelerate and enhance the research productivity of the JAHVH investigators, allow for development of new research directions, increase synergy between investigators, and increase the translational research capacity of the JAHVH Research Program. Overall, the proposed instrument will significantly impact and augment VAMC's mission to advance the healthcare of Veterans by allowing VA researchers to conduct cutting-edge research in basic, translational or clinical fields. These endeavors will substantially contribute to the long-term research goals of the JAHVH.

该提案请求 KEYENCE BZ-X810 荧光显微镜作为公共资源 共享核心实验室设施的主要设备，供生物医学研究人员使用 詹姆斯·A·哈利退伍军人医院 (JAHVH)。 JAHVH 的生物医学研究由 知名且资金充足的研究人员。拟议的工具将主要由 12 研究人员，包括 2 名高级研究职业科学家和 2 名研究职业科学家 正在进行的与阿尔茨海默病、癌症、糖尿病、肥胖症、海湾地区相关的研究项目 战争病、呼吸道合胞病、脑外伤和再生医学。在 此外，这些个人还拥有其他拨款（NIH、AHA、FLDOH、DoD 等），这些拨款将利用 荧光显微镜有助于建立现有和新的研究项目，从而受益 退伍军人。 此次收购将是对已有 15 年以上停业历史的急需的替代品。 目前可以使用显微镜。没有其他多功能荧光显微镜 JAHVH 内提供，南佛罗里达大学（USF，我们的 附属大学）在 USF 内需求量很大，由 5 所学院共享，而且通常时间段都 由于 VA 研究人员不比 USF 研究人员享有优先权，因此不可用。此外， 运送到 USF 会造成延误，对样品产生不利影响。 这款荧光显微镜有很多优点： 尺寸紧凑，节省台面空间；简单的 所有用户均可操作；灵敏的光学器件可提供高质量的结果；指定测量范围； 自动批量采集所需图像；条件统一的高内涵分析； 具有高分辨率拼接和 Z 堆叠的多维图像捕获；结构化 照明消除了荧光模糊，只需轻轻一按即可提供清晰的图像；捕获 通过记录其最外层位置的坐标，自动生成整个样本； 精确分析结构和 3D 信号。 多功能荧光显微镜以更高的分辨率捕获图像 与传统显微镜相比的灵敏度。多功能一体、暗室 不需要。它可以同时扫描多张幻灯片。该显微镜的其他优点 包括增强的信噪比、对厚样品的可理解和仔细的检查， 高质量图像是研究项目的关键，也是高水平出版物的核心 影响期刊并获得联邦资助。此外，仪器仪表将加速并 提高 JAHVH 研究人员的研究生产力，允许开发新的 研究方向，增加研究者之间的协同作用，并增加转化 JAHVH 研究计划的研究能力。 总体而言，拟议的文书将显着影响并增强 VAMC 的使命： 通过允许退伍军人管理局研究人员进行尖端研究来促进退伍军人的医疗保健 基础、转化或临床领域的研究。这些努力将大大有助于 JAHVH 的长期研究目标。