An Artificial Eggshell Membrane for Guided Tissue Regeneration

用于引导组织再生的人造蛋壳膜

• 批准号: 9896507
• 负责人: Yuanyuan Lucy Duan
• 金额: $ 15.5万
• 依托单位: UNIVERSITY OF MISSISSIPPI MED CTR
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2022-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9896507
• 关键词: Adult; Animal Model; Behavior; Biocompatible Materials; Biomimetics; Birds; Bone Regeneration; Bone Tissue; Cell Adhesion; Cell Proliferation; Cells; Chronic; Clinical; Connective Tissue; Craniofacial Abnormalities; Data; Defect; Dental; Dentistry; Egg White; Electrospinning; Embryonic Development; Environment; Epithelial; Epithelium; Evaluation; Fiber; Food Industry; Foundations; Generations; Gingiva; Glycolates; Goals; Guided Tissue Regeneration; Hydroxyapatites; In Situ; In Vitro; Individual; Industrial Product; Inflammatory; Ligaments; Liquid substance; Mechanics; Medicine; Membrane; Membrane Proteins; Methodology; Molecular; Morphology; Outcome; Oxygen; Periodontal Diseases; Periodontal Ligament; Permeability; Play; Polymers; Population; Poultry Products; Preparation; Process; Proteins; Role; Signal Transduction; Structure; Surface; System; Techniques; Tissues; Tooth Loss; Waste Products; Water; base; biomineralization; bone; bone loss; cell behavior; clinical predictors; cost; craniofacial bone; crosslink; improved; in vivo evaluation; innovation; mechanical properties; microorganism; migration; nano; nanofiber; novel; orofacial; particle; prevent; response; tissue regeneration

Project Summary Periodontal disease is a chronic inflammatory process in the periodontal tissue. It occurs in approximately 48% of the U.S. adult population and is the leading cause of tooth loss among adults. Guided tissue generation (GTR) is a widely-used, well-documented and clinically predictable technique to treat periodontal disease and dental/craniofacial defects by placing a polymeric membrane between epithelial tissue and periodontal ligament/bone tissue. This membrane serves as a barrier to protect the slower-growing periodontal ligament and bone tissues from the invasion of faster-growing epithelial tissue, but it should also allow exchange of fluids and signals between different tissues. A number of barrier membranes have been developed over the past decades, but current products are very costly and have limitations. Avian eggshell membrane is a semi-permeable fibrous membrane between egg-white and eggshell. It is available in abundant quantities as a waste product from the poultry and food industry. Natural eggshell membrane is composed of a highly-crosslinked interwoven protein fiber network with a unique double-layered structure. It not only allows excellent water/oxygen exchange and satisfies the need of embryo development, but also serves as a natural barrier membrane against the invasion of microorganisms and other challenges from the external environment. Inspired by the similarity of eggshell membrane and GTR membrane as a barrier membrane, we propose this project to explore the feasibility of developing an innovative and biomimetic artificial eggshell membrane for GTR application using a reactive electrospinning technique and response surface methodology (RSM), which can simulate the unique composition and microstructure of natural eggshell membrane. Our new membrane is a multiple-component system which contains soluble eggshell membrane protein (SEP), mechanically stable poly (lactic-co-glycolic acid) (PLGA) and osseoinductive nano-hydroxyapatite particles (nanoHAp). It also possesses a bio- inspired double-layered structure, which includes an outer barrier PLGA layer (adjacent to the gingival tissue) and an inner bioactive SEP/PLGA/nano-HAp layer (adjacent to the periodontal/bone tissue). The long-term goal of our proposed project is to improve the clinical outcome of the GTR technique by using this biomimetic membrane, as well as lay a foundation for converting eggshell membrane derived materials from an abundant industrial by-product to a new, green and promising biomaterial for dentistry and medicine.

项目概要 牙周病是牙周组织的慢性炎症过程。它发生在 约占美国成年人口的 48%，是导致牙齿脱落的主要原因 成年人。引导组织生成 (GTR) 是一种广泛使用、有据可查的临床方法 通过放置一个可预测的技术来治疗牙周病和牙齿/颅面缺陷 上皮组织和牙周膜/骨组织之间的聚合物膜。这 膜作为屏障，保护生长缓慢的牙周韧带和骨骼 组织免受生长较快的上皮组织的侵袭，但它也应该允许交换 不同组织之间的液体和信号。已有多种阻隔膜 经过过去几十年的发展，但目前的产品成本非常高并且有局限性。禽类 蛋壳膜是介于蛋清和蛋壳之间的半透性纤维膜。它 作为家禽和食品工业的废物，其数量非常丰富。自然的 蛋壳膜由高度交联的交织蛋白纤维网络组成 独特的双层结构。它不仅可以实现良好的水/氧交换并满足 胚胎发育的需要，同时也作为对抗 微生物的入侵以及来自外部环境的其他挑战。 受到蛋壳膜和 GTR 膜作为屏障膜的相似性的启发，我们 提出该项目旨在探索开发创新型仿生人工体的可行性 使用反应静电纺丝技术和响应进行 GTR 应用的蛋壳膜 表面方法（RSM），可以模拟独特的成分和微观结构 天然蛋壳膜。我们的新膜是一个多组分系统，其中包含 可溶性蛋壳膜蛋白（SEP）、机械稳定的聚（乳酸-乙醇酸） （PLGA）和骨诱导纳米羟基磷灰石颗粒（nanoHAp）。它还拥有生物 受启发的双层结构，其中包括外部阻挡层 PLGA 层（邻近 牙龈组织）和内部生物活性 SEP/PLGA/nano-HAp 层（邻近牙龈组织） 牙周/骨组织）。我们提出的项目的长期目标是改善临床 使用这种仿生膜的GTR技术的成果，并奠定基础 用于将蛋壳膜衍生材料从丰富的工业副产品转化为 用于牙科和医学的新型、绿色和有前途的生物材料。