Ocular Phenotyping Core

眼表型核心

• 批准号: 9767150
• 负责人: Timothy W Kraft
• 金额: $ 7.29万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9767150
• 关键词: Anatomy; Angiography; Animals; Anterior; Area; Award; Basic Science; Behavior; Biological Assay; Collaborations; Color; Communities; Contrast Sensitivity; Core Facility; Disease; Doctor of Philosophy; Dyes; Electroretinography; Environment; Equipment; Eye; Funding; Fundus; Fundus photography; Health; Human; Human Resources; Image; Imagery; Institution; Laboratories; Lead; Magnetic Resonance Imaging; Microscopy; Monitor; Mus; National Center for Research Resources; Ocular Physiology; Optical Coherence Tomography; Optokinetic nystagmus; Outcome; Outcome Measure; Phenotype; Productivity; Publications; Research; Research Personnel; Research Support; Retinal; Rodent; Scientist; Signal Transduction; Standardization; Structure; Surveys; Technology; Tissues; Training; Training Support; Translational Research; Validation; Vision; Visual; Visual Acuity; Visual Perception; Zebrafish; base; behavior test; body system; college; design; digital; equipment acquisition; experience; fundus imaging; instrument; instrumentation; interest; member; mouse model; multimodality; novel diagnostics; operation; release of sequestered calcium ion into cytoplasm; research facility; response; satisfaction; skills; slit lamp imaging; success; tomography; vision science; visual motor

Ocular Phenotyping Core 7. Project/Summary Abstract Non-invasive assessment of eye structure and function is essential to both basic and translational research in vision science. Widely used technologies include slit lamp for biomicroscopy of anterior and posterior segment, electroretinography (ERG, for massed retinal signal separable into components), optical coherence tomography (OCT) for display of layered tissues in posterior and anterior segments, imaging of the fundus via multiple modes of visualization (color, autofluorescence, dye-based angiography, infrared reflectance), and optokinetic nystagmus (to assess visuomotor control, visual acuity and contrast sensitivity). In response to growing UAB vision researcher needs, a new “Ocular Phenotyping Core” was established to encompass a comprehensive suite of instrumentation and to provide the necessary support for a qualified PhD staff member to assist in accurate ocular phenotyping. Specific instruments include Bioptigen 840 nm SD-OCT and Micron IV digital fundus camera for small animals, Spectralis SDOCT for large animals and human donor eyes, and Optomotry optokinetic nystagmus in small animals including mice and zebrafish. This core will support 15 UAB Vision Scientists, including 13 with planned moderate to extensive use and 9 who are currently NEI R01-funded. Review of publications during the two years prior to this application reveals excellent productivity using these collaboratively purchased instruments, justifying the formalizing of these activities as a core. The Director and Associate Director of this proposed core have extensive publication experience in electroretinography and OCT validation/ interpretation, respectively. New directions for the core include the establishment of MRI imaging for calcium flux in rodent eyes. Additionally an “Ocular Phenotyping Fair” will be implemented to identify new ocular mouse models through full ocular phenotyping screens of mouse models generated by non-ocular scientists that were originally generated to answer questions pertinent to thier organ systems of interest.

眼表型核心 7。项目/摘要摘要 对眼睛结构和功能的非侵入性评估对于基本和翻译研究至关重要 视觉科学。广泛使用的技术包括用于前部和后段的生物显微镜的缝隙灯， 电视图（ERG，对于可分离为组件的质量视网膜信号），光学相干断层扫描 （OCT）用于在后段和前部显示分层组织，通过多种模式对眼底进行成像 可视化（颜色，自动荧光，基于染料的血管造影，红外反射率）和光动量 眼球震颤（评估视觉运动控制，视力和对比度灵敏度）。响应不断增长的UAB 视觉研究人员的需求，建立了一种新的“眼表型核心”，以涵盖一个全面的 仪器套件，并为合格的博士工作人员提供必要的支持以协助 准确的眼表型。特定仪器包括Bioptigen 840 nm SD-OCT和Micron IV数字 小动物的眼底摄像头，用于大动物的光谱SDOCT和人类捐赠者的眼睛以及验光 小动物中的光动力学无关，包括小鼠和斑马鱼。该核心将支持15 UAB视觉 科学家，包括有计划现代化的13名与广泛使用的现代化，目前是NEI R01资助的9人。审查 在此应用程序之前的两年中，出版物的出版物揭示了使用这些出版物的出色生产力 合作购买了工具，证明这些活动的格式为核心。导演和 该拟议核心的副主任在电视图和OCT方面具有丰富的出版经验 验证/解释分别。核心的新方向包括建立MRI成像 啮齿动物的钙通量。此外，将实施“眼表型公平”以识别新的眼 小鼠模型通过非眼科学家产生的小鼠模型的全眼表型筛选 最初是为了回答与您的感兴趣的器官系统有关的问题。