Host-Microbial Analytic and Repository Core

宿主微生物分析和储存库核心

基本信息

批准号：
9762893
负责人：
GARY D. WU
金额：
$ 18.17万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2019
资助国家：
美国
起止时间：
2019-07-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9762893
关键词：
Address Animal Model Bioinformatics Biological Models Biometry Clinical Clinical Data Collection Computerized Medical Record Consult DNA Sequencing Facility Data Set Development Digestive System Disorders Disease Environmental Risk Factor Epigenetic Process Evaluation Funding Gene Expression Generations Genes Genomics Human Human Biology Human Microbiome Human Subject Research Immune response In Vitro Intervention Intervention Studies Intestines Investments Joints Lead Liver diseases Mammalian Cell Messenger RNA Metadata Microbe Microbial Taxonomy Mind Modality Modification Molecular Molecular Biology Mucosal Immunity Pancreatic Diseases Pathogenesis Pediatric Hospitals Pennsylvania Phenotype Philadelphia Physiology Play Population Production Property Proteins Research Role Services Shotguns System Testing Time Translating Translations Universities base design experimental study gut microbiome gut microbiota host-microbe interactions human subject instrument interest member metabolome metabolomics metagenomic sequencing microbial microbial host microbiome microbiome analysis microbiota mouse model next generation sequencing potential biomarker pre-clinical pre-clinical research precision medicine prevent programs repository response success transcriptomics

Address Animal Model Bioinformatics Biological Models Biometry Clinical Clinical Data Collection Computerized Medical Record Consult DNA Sequencing Facility Data Set Development Digestive System Disorders Disease Environmental Risk Factor Epigenetic Process Evaluation Funding Gene Expression Generations Genes Genomics Human Human Biology Human Microbiome Human Subject Research Immune response In Vitro Intervention Intervention Studies Intestines Investments Joints Lead Liver diseases Mammalian Cell Messenger RNA Metadata Microbe Microbial Taxonomy Mind Modality Modification Molecular Molecular Biology Mucosal Immunity Pancreatic Diseases Pathogenesis Pediatric Hospitals Pennsylvania Phenotype Philadelphia Physiology Play Population Production Property Proteins Research Role Services Shotguns System Testing Time Translating Translations Universities base design experimental study gut microbiome gut microbiota host-microbe interactions human subject instrument interest member metabolome metabolomics metagenomic sequencing microbial microbial host microbiome microbiome analysis microbiota mouse model next generation sequencing potential biomarker pre-clinical pre-clinical research precision medicine prevent programs repository response success transcriptomics

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项目摘要

PROJECT SUMMARY (HOST-MICROBIAL ANALYTICS & REPOSITORY CORE) There is growing evidence for the importance of environmental factors, such as the microbiome, playing a critical role in the pathogenesis of many digestive, liver and pancreatic diseases. There is much interest in the characterization of the microbiome to identify potential biomarkers relevant for precision medicine as well as the modification of the human microbiome as a modality to prevent and/or treat diseases. To facilitate research focused on host-microbial interactions and their relevance to digestive, liver and pancreatic diseases, the Center for Molecular Studies in Digestive and Liver Diseases (CMSDLD) has developed a Host-Microbial Analytic and Repository Core (H-MARC) with the following two Specific Aims: 1) To provide critical analytic services the characterize analytes (i.e. genomics, transcriptomics, metabolomics) in both microbes and their mammalian hosts and 2) To provide expertise that will allow CMSDLD members to extend pre-clinical in vitro and animal model research into the human clinical domain. In Specific Aim 1, to support the analysis of the mammalian host, H-MARC will provide access to high-end instruments designed to quantify gene expression at the mRNA and protein levels as well as access to FACS for the characterization of mammalian cell populations. Genomic analysis will be provided via Penn's Next Gen Sequencing Core. To support the analysis of the microbiota, H-MARC will support experiments involving microbial cultures as well as the analysis of metabolites via targeted metabolomics. Computational and biostatistical support to analyze microbiome datasets associated with clinical metadata will also be provided through full time biostatisticians in H-MARC who will interface with the PennCHOP Microbiome Program. In Specific Aim 2, H-MARC will support human subject research by providing a robust Human Biospecimen Repository via a LabVantage-based LIMS system as well as the collection of robust annotated clinical data for IBD phenotyping via the EPIC EMR. Importantly, to facilitate human subject research translating preclinical research into the clinical domain, H-MARC will provide advice and expertise in the development of human intervention studies. Ultimately, H-MARC services are designed to facilitate integrated analyses of host-microbial interactions at the preclinical and clinical interface.

项目摘要（主机 - 微生物分析和存储库核心）越来越多的证据证明环境因素的重要性，例如微生物组，在许多消化，肝脏和胰腺疾病的发病机理中的关键作用。对微生物组的表征以识别与精度医学相关的潜在生物标志物以及人类微生物组的修改为预防和/或治疗疾病的方式。促进研究重点是宿主 - 微生物相互作用及其与消化，肝脏和胰腺疾病的相关性，消化和肝病分子研究中心（CMSDLD）开发了一种宿主微生物分析和存储库核（H-MARC）具有以下两个具体目的：1）提供关键的分析服务在微生物及其的特征表征分析物（即基因组学，转录组学，代谢组学）哺乳动物宿主和2）提供专业知识，使CMSDLD成员可以扩展临床前体外动物模型研究人类临床领域。在特定目标1中，支持分析哺乳动物宿主H-MARC将提供对旨在量化基因表达的高端仪器的访问权限在mRNA和蛋白质水平以及进入哺乳动物细胞表征的FACS 人群。基因组分析将通过宾夕法尼亚州的下一个一代测序核心提供。支持分析在微生物群中，H-marc将支持涉及微生物培养物的实验以及分析代谢物通过靶向代谢组学。计算和生物统计支持以分析微生物组与临床元数据相关的数据集也将通过H-Marc的全职生物统计学家提供谁将与Pennchop Microbiome程序进行交互。在特定的目标2中，H-Marc将支持人类主题研究通过基于LabVantage的LIMS系统提供强大的人类生物循环库以及通过EPIC EMR收集IBD表型的可靠注释的临床数据。重要的是，为了促进将临床前研究转化为临床领域的人类主题研究，H-Marc将在人类干预研究的发展方面提供建议和专业知识。最终，H-MARC服务旨在促进临床前和临床上宿主微生物相互作用的综合分析界面。