Mechanisms behind Electrode Induced BBB damage's impact on neural recording

电极诱导 BBB 损伤对神经记录影响的机制

• 批准号: 9760009
• 负责人: Takashi Daniel Yoshida Kozai
• 金额: $ 39.04万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-01 至 2021-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9760009
• 关键词: 3-Dimensional; Acute; Albumins; Alzheimer' s Disease; Aneurysm; Architecture; Area; Arteries; Autopsy; Basic Science; Beds; Blood; Blood - brain barrier anatomy; Blood Vessels; Blood capillaries; Brain; Brain Injuries; Caliber; Cerebral Ischemia-Hypoxia; Chronic; Cicatrix; Clinical Sciences; Cortical Vein; Data; Deposition; Devices; Dyes; Electrodes; Electrophysiology (science); Engineering; Extravasation; Failure; Fibrinogen; Functional disorder; Future; Genetic; Geometry; Goals; Health; Hemorrhage; Human; Hypoxia; Image; Imaging technology; Immunohistochemistry; Implant; Implanted Electrodes; Individual; Inflammatory; Injury; Intervention; Intracranial Hemorrhages; Ischemia; Knowledge; Lead; Learning; Limb structure; Literature; Maps; Mechanics; Memory; Microelectrodes; Molecular; Motor; Multiple Sclerosis; Needles; Neurons; Neurosciences; Noise; Nutrient; Outcome; Output; Oxygen; Patients; Performance; Perfusion; Prevalence; Quadriplegia; Reperfusion Therapy; Role; Signal Transduction; Site; Source; Spectrum Analysis; Stroke; Structure; Surface; Technology; Testing; Time; Tissues; Trauma; Traumatic Brain Injury; Universities; Veins; Visual Cortex; Waste Products; Work; angiogenesis; blind; blood perfusion; blood treatment; brain computer interface; calcium indicator; cranium; design; electric impedance; healing; implantation; improved; in vivo; in vivo imaging; innovation; insight; multiphoton imaging; nervous system disorder; neural implant; neuron loss; neuroprosthesis; neurotoxic; pressure; public health relevance; relating to nervous system; repaired; response; robot control; success; treatment strategy; two-photon

 DESCRIPTION (provided by applicant): Penetrating recording microelectrode arrays are a crucial component of numerous human neuroprosthetics. Obtaining selective, high fidelity, long-lasting readouts of brain activity is a critical technology across basic and applied neuroscience that impacts learning and memory studies as well as motor, pre-motor, and visual cortex neuroprostheses and brain-computer interfaces. However, implantation of cortical microelectrodes causes a reactive tissue response, which results in a degradation of the preferred functional single-unit performance over time, thus limiting the device capabilities. Insertion of neural probes or microelectrodes inevitably disrupts the blood-brain barrier (BBB) integrity and causes microhemorrhages that have been shown to trigger the inflammatory tissue response cascade. The degree of microhemorrhaging from probe insertion has been shown to be uncontrollable and difficult to reproduce across implants, mirroring the large variability in inflammatory tissue responses and chronic recording success. We hypothesize that the level of BBB damage impacts chronic neural recording quality. This proposal aims to characterize the sustained BBB breakdown and chronic recording failure in vivo caused by the insertion induced BBB disruption and BBB occlusion by quantifying structural, cellular, and molecular level tissue response to chronic implants in the brain in real time through combining multiphoton imaging technology and neural engineering technology at the University of Pittsburgh. A dynamic understanding of the interfaces is necessary for elucidating the mechanism(s) behind neural recording failure. This work has the potential to output basic and clinical science level knowledge relevant to neural engineering, ischemia, stroke, intracortical hemorrhage, aneurysm, traumatic brain injury, and closed-loop neurostimulation.

 描述（由应用程序提供）：穿透记录微电极阵列是许多人类神经假想的关键组成部分。在基本和应用的神经科学中，获得选择性，高保真，持久的读数是一项关键技术，它影响学习和记忆研究以及运动，运动前和视觉皮层神经前体和脑部计算机界面。然而，皮质微电极的植入会导致反应性组织反应，从而导致优选的功能单单元性能随着时间的推移而降解，从而限制了设备的功能。神经元问题或微电极的插入不可避免地破坏了血脑屏障（BBB）完整性并引起微移位，这些微视力已被证明会触发炎症组织反应级联反应。探测插入的微释放程度已被证明是无法控制的，并且在处面之间难以繁殖，这反映了炎症组织反应的较大差异和慢性记录成功。我们假设BBB损害的水平会影响慢性神经记录质量。该提议旨在表征由插入引起的BBB诱导的BBB破坏和慢性记录失败，通过量化结构，细胞和分子水平组织对实时的慢性不含的结构，通过结合多音像技术和神经工程技术的技术，通过量化结构，细胞和分子水平组织对大脑中慢性不屑一顾的响应，引起了BBB的破坏和BBB的闭塞。对界面的动态理解对于阐明神经元记录失败背后的机制是必要的。这项工作有可能输出与神经工程，缺血，中风，心脏内出血，动脉瘤，创伤性脑损伤和闭环神经刺激相关的基本和临床科学水平知识。