Dissecting the circuits that support high acuity spatial vision

剖析支持高敏锐度空间视觉的电路

• 批准号: 9752625
• 负责人: Dario Xavier Figueroa Velez
• 金额: $ 4.07万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9752625
• 关键词: Affect; Amblyopia; Animals; Behavior; Calcium; Child; Childhood; Data; Development; Disease; Frequencies; Goals; Image; Impairment; Interneurons; Lead; Mus; Neurodevelopmental Disorder; Neurons; Ocular Dominance; Perception; Population; Recovery; Reporting; Research; Stimulus; Sum; Testing; Transplantation; Vision; Vision Disorders; Visual; Visual Acuity; Visual Cortex; Visual Perception; Visual impairment; Water; Work; critical period; deprivation; in vivo calcium imaging; insight; neural circuit; neuromechanism; novel; postnatal; postnatal development; relating to nervous system; response; restoration; sensory cortex; spatial vision; two-photon; visual deprivation

PROJECT SUMMARY Amblyopia is a disorder of visual acuity caused by disruptions in vision during an early critical period. The neural basis for amblyopia has predominantly been studied using ocular dominance plasticity. I recently discovered a distinct critical period for high spatial acuity in visual cortex that precedes the ocular dominance critical period. In this proposal, I aim to take advantage of this key insight and dissect out the specific neural circuits responsible for high acuity responses. Using in vivo calcium imaging of large populations of cortical neurons, I will determine the development of high spatial frequency tuned neurons in mouse visual cortex. I hypothesize that the emergence of high acuity vision involves the emergence of a subpopulation of high spatial frequency tuned neurons. Previously, I showed inhibitory interneuron transplantation induces recovery of high acuity vision in amblyopic mice. I will use a calcium imaging to determine whether our previously reported transplant induced restoration of acuity in amblyopic mice involves the restoration of high spatial frequency tuned neurons. If successful, this work will be provide insight into the neural circuits underlying high acuity vision and may lead to new insight for amblyopia research.!

项目概要 弱视是一种因早期关键时期视力受损而引起的视力障碍。神经元 主要利用眼优势可塑性来研究弱视的基础。我最近发现了一个 视觉皮层高空间敏锐度的独特关键期先于眼优势关键期。 在这个提案中，我的目标是利用这一关键见解并剖析负责的特定神经回路 用于高敏锐度反应。使用大量皮质神经元的体内钙成像，我将确定 小鼠视觉皮层高空间频率调谐神经元的发育。我假设 高敏锐度视觉的出现涉及高空间频率调谐亚群的出现 神经元。此前，我表明抑制性中间神经元移植可诱导高视力恢复 弱视小鼠。我将使用钙成像来确定我们之前报道的移植是否诱导 弱视小鼠视力的恢复涉及高空间频率调谐神经元的恢复。如果 如果成功的话，这项工作将深入了解高敏锐度视觉背后的神经回路，并可能导致 弱视研究的新见解！