Molecular and Cellular Analytical Core

分子和细胞分析核心

基本信息

批准号：
9750763
负责人：
JACOB E FRIEDMAN
金额：
$ 17.37万
依托单位：
UNIVERSITY OF COLORADO DENVER
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/9750763
关键词：
Adipocytes Adipose tissue Animals Award Back Basic Science Biological Assay Biological Metamorphosis Biology Body Composition Brain Calibration Caring Cell Nucleus Cell Separation Cell Size Cells Charge Clinical Clinical Research Colorado Communities Consultations Data Analyses Diabetes Mellitus Disease Energy Metabolism Equipment Evolution Experimental Designs Exposure to Fatty acid glycerol esters Gene Expression Regulation Genus Hippocampus Grant Hormones Human Human Milk Human Resources Hybrids Immune Individual Infiltration Inflammation Insulin Interdisciplinary Study Investigation Laboratories Lipolysis Liquid substance Liver Maintenance Measures Medical Metabolic Metabolism Mission Mitochondria Modernization Molecular Muscle Nutritional Study Obesity Obesity associated disease Outcome Pathology Phenotype Plasma Procedures Protein Analysis Quality Control Quantitative Reverse Transcriptase PCR Reagent Research Research Personnel Respiration Schedule Services Sorting - Cell Movement Standardization System Techniques Technology Time Tissues Training Translational Research adipokines analytical tool animal tissue base cost cytokine energy balance experience extracellular fetal human tissue instrumentation meetings member mouse model nutrition quality assurance tool

项目摘要

Project Summary – Molecular and Cellular Analytical Core The overall theme of the Molecular and Cellular Analytical (MCA) core is to support investigation of human and non-human metabolism from a cellular/molecular perspective. The overall expertise of the PI in obesity, energy balance, inflammation, and maternal-fetal metabolism provides the nucleus of expertise around which this core lab has been formed. The Core provides analysis and consultation on hormone assays, qPCR, mitochondria function, and cell based assays of cytokines, adipokines, and immune cell isolation tailored to the investigators needs. Over the last grant period the Molecular and Cellular Analytical Core has developed expertise in use of the Seahorse XF24-3 extracellular flux analyzer to allow us to interrogate mitochondrial function. The Molecular and Cellular Analytical Core continues to provide a broad array of assays over a variety of tissue types (fat, muscle, liver, brain), fluids (plasma, media, breast milk), and cells allowing for high throughput for >40 investigators studying nutrition and obesity-related disorders. The Molecular and Cellular Analytical Core lab also provides members of the Center with reagents, access to equipment, and exposure to new techniques (Seahorse technology, cytokine analysis, adipocyte isolation) that have helped to expand the quantity and quality of nutrition research in the NORC.

项目摘要 - 分子和细胞分析核心分子和细胞分析（MCA）核心的总体主题是支持研究从细胞/分子的角度来看人类和非人类代谢。总体 PI在肥胖，能量平衡，感染和母亲代谢方面的专业知识提供了围绕该核心实验室形成的专业知识的核心。核心提供有关Horseneses分析，QPCR，线粒体功能和细胞的分析和咨询针对研究人员量身定制的细胞因子，脂肪因子和免疫细胞隔离的基于基础测定需要。在最后一个赠款期间，分子和细胞分析核心已经发展使用Seahorse XF24-3细胞外通量分析仪的专业知识，使我们能够询问线粒体功能。分子和细胞分析核心继续提供广泛的各种组织类型（脂肪，肌肉，肝脏，大脑），流体（等离子体，培养基，，，培养基，母乳），细胞允许40位研究营养和研究人员进行高吞吐量与肥胖有关的疾病。分子和细胞分析核心实验室也提供中心成员，具有试剂，使用设备以及接触新技术的成员（海马技术，细胞因子分析，脂肪细胞隔离），有助于扩大 NORC营养研究的数量和质量。