Methylmercury Exposure Through Rice Ingestion, Gut Microbes, and Offspring Development

通过稻米摄入、肠道微生物和后代发育而接触甲基汞

• 批准号: 9552634
• 负责人: Sarah E Rothenberg
• 金额: $ 6.3万
• 依托单位: OREGON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-16 至 2020-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9552634
• 关键词: Methylmercury; Exposure; Through; Rice; Ingestion

 DESCRIPTION (provided by applicant): Mercury is a known neurotoxin; methylmercury is one of the most potent forms due to its ability to cross both the blood-brain and placental barriers. Unlike less toxic inorganic mercury, about 95% of dietary methylmercury is absorbed into the bloodstream, entering the hepatic portal circulation. The long, biological half-time of methylmercury (45-70 days) is attributed to extensive enterohepatic recycling, which is interrupted when methylmercury is demethylated by gut microbiota and excreted in feces. Thus gut microbial function is important to detoxification via its role in human metabolism and elimination of methylmercury. Conversely, evidence from human studies indicates not all methylmercury in the gut is demethylated, while inorganic Hg methylation by an archeon (isolated from human feces) is also possible. Inorganic mercury (or methylmercury) exposure induced changes in gut microbe communities; however data were limited to studies involving monkeys, crustaceans, and earthworms. Human studies are needed to establish bi-directional associations between gut microbiota, dietary methylmercury intake, and accumulation of methylmercury in tissues. To address this knowledge gap, we will leverage our prospective birth and child cohort study in China, to investigate associations between prenatal/postnatal methylmercury exposure and gut microbiota assessed in children's stools. The following includes the specific aims: Aim 1. Establish the impacts of prenatal and postnatal methylmercury exposure through rice ingestion on children's neurodevelopment, evaluated using the Bayley Scales of Infant Development. Aim 2. Assess the relative taxonomic abundance and diversity of gut microbiota (phylum, class, order, family, and genus levels) in children's stool samples using 16S rRNA gene profiling, to investigate associations between gut microbes, methylmercury exposure (prenatal/postnatal), and other confounders. Aim 3. Establish whether variability in methylmercury metabolism and exposure corresponds to microbial detoxification via mercury resistance genes (merA and merB) quantified in children's stool samples. Results from this study will clarify our understanding of the mechanisms by which gut microbes contribute to variability in children's methylmercury metabolism and exposure, potentially impacting offspring development.

 描述（由申请人提供）：汞是一种已知的神经毒素；甲基汞是最有效的形式之一，因为它能够穿过血脑屏障和胎盘屏障，与毒性较低的无机汞不同，大约 95% 的膳食甲基汞被吸收。进入血液，进入肝门循环，甲基汞的生物半衰期长（45-70天）归因于广泛的肠肝。当甲基汞被肠道微生物群去甲基化并通过粪便排出时，其循环就会被中断。因此，肠道微生物功能通过其在人体代谢和消除甲基汞中的作用对于解毒很重要，来自人类的研究表明，并非肠道中的所有甲基汞都是如此。去甲基化，而古菌（从人类粪便中分离出来）对无机汞的甲基化也是可能的。甲基汞）暴露引起肠道微生物群落的变化；然而，数据仅限于涉及猴子、甲壳类动物和蚯蚓的研究，以建立肠道微生物群、膳食甲基汞摄入量和组织中甲基汞积累之间的双向关联。为了解决这一知识差距，我们将利用在中国的前瞻性出生和儿童队列研究来调查产前/产后甲基汞暴露与儿童粪便中评估的肠道微生物群之间的关联。包括具体目标： 目标 1. 确定通过摄入大米接触产前和产后甲基汞对儿童神经发育的影响，并使用婴儿发育贝利量表进行评估 目标 2. 评估肠道微生物群（门、纲）的相对分类丰度和多样性。使用 16S rRNA 基因分析对儿童粪便样本中的（目、科和属水平）进行研究，以研究肠道微生物与甲基汞之间的关联目标 3. 确定甲基汞代谢和暴露的变异是否与通过儿童粪便样本中的汞抗性基因（merA 和 merB）量化的微生物解毒相对应。本研究的结果将澄清我们对这一问题的理解。肠道微生物导致儿童甲基汞代谢和暴露变异的机制，可能影响后代的发育。

项目成果

Sewage spills are a major source of titanium dioxide engineered (nano)-particle release into the environment

• DOI: 10.1039/c8en01376d
• 发表时间: 2019-03-01
• 期刊: ENVIRONMENTAL SCIENCE-NANO
• 影响因子: 7.3
• 作者: Loosli, Frederic;Wang, Jingjing;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed

Enrichments of Metals, Including Methylmercury, in Sewage Spills in South Carolina, USA.

• DOI: 10.2134/jeq2018.02.0067
• 发表时间: 2018-09
• 期刊: Journal of environmental quality
• 影响因子: 2.4
• 作者: Emmons AM;Bizimis M;Lang SQ;Stangler W;Geidel G;Baalousha M;Wanamaker E;Rothenberg SE
• 通讯作者: Rothenberg SE

Maternal methylmercury exposure through rice ingestion and child neurodevelopment in the first three years: a prospective cohort study in rural China.

• DOI: 10.1186/s12940-021-00732-z
• 发表时间: 2021-04-28
• 期刊: Environmental health : a global access science source
• 作者: Rothenberg SE;Korrick SA;Liu J;Nong Y;Nong H;Hong C;Trinh EP;Jiang X;Biasini FJ;Ouyang F
• 通讯作者: Ouyang F

Neurodevelopment correlates with gut microbiota in a cross-sectional analysis of children at 3 years of age in rural China.

• DOI: 10.1038/s41598-021-86761-7
• 发表时间: 2021-04-01
• 期刊: Scientific reports
• 影响因子: 4.6
• 作者: Rothenberg SE;Chen Q;Shen J;Nong Y;Nong H;Trinh EP;Biasini FJ;Liu J;Zeng X;Zou Y;Ouyang F;Korrick SA
• 通讯作者: Korrick SA