Molecular and Antibody Detection of Zika Virus in Saliva at the Point of Care

护理点唾液中寨卡病毒的分子和抗体检测

基本信息

批准号：
9350279
负责人：
Haim H Bau
金额：
$ 20.13万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-09-09 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9350279
关键词：
Address Affinity Americas Antibodies Area Biological Assay Blood Body Fluids Brazil Cellular Phone Centers for Disease Control and Prevention (U.S.)Central America Centrifugation Characteristics Clinic Communication Culicidae Custom Data Dengue Virus Dentists Detection Development Devices Diagnostic Diagnostic tests Disease Outbreaks Enzyme-Linked Immunosorbent Assay FDA approved Fetal Development Fetus Flavivirus Infections Gold Health Heating Home environment Hybrids Immunoassay Individual Infection International Label Laboratories Liquid substance Mediating Methods Microcephaly Microfluidic Microchips Microfluidics Modification Molecular Monitor Neurologic Nucleic Acids Oral Panama Patients Pennsylvania Performance Point-of-Care Systems Pregnancy Pregnant Women Reaction Reporting Research Resources Reverse Transcriptase Polymerase Chain Reaction Reverse Transcription Risk Management Safety Saliva Sampling Seasons Serum Signal Transduction South America Specificity Symptoms System Technology Test Result Testing Time Treatment Efficacy Universities Urine Vascular blood supply Viral Viral Antigens Viremia Virus Diseases Visual Work Zika Virus base cost cross reactivity design improved instrument instrumentation laboratory facility molecular diagnostics pandemic disease pathogen point of care point-of-care diagnostics public health emergency seroconversion therapy development tool vaccine development vaccine efficacy viral detection

项目摘要

ABSTRACT Zika virus (ZIKV) infection with its devastating impact on neurological development of fetuses is already a major health problem in South and Central America, and is likely to impact parts of the USA. Reliable diagnostic tools for ZIKV are vital since ZIKV-infected individuals display either no symptoms or nonspecific symptoms similar to other, more common, viral infections. These diagnostic tools are also essential for management of risks during pregnancy; assessing efficacy of vaccines and therapies; tracking the spread of the infection, tracking ZIKV effects on fetal development; assisting in eradication efforts; and assuring the safety of blood supply. Currently, according to the CDC, no reliable diagnostic tools for ZIKV are available and pipeline technologies are mostly focused on methods suitable for centralized laboratories and inappropriate for resource poor settings and the point of care. This collaborative effort between the University of Pennsylvania and the Instituto Conmemorativo Gorgas de Estudios de la Salud (ICGES) in Panama will research and develop a hybrid, inexpensive, point of care system for combined molecular detection of ZIKV and of antibodies against the ZIKV in oral samples. The proposed system can be used in the clinic, doctor and dentist office, and at home with minimal instrumentation and (optionally) without electrical power. To reduce cost, enable quantification, the system utilizes a smartphone for signal detection, processing, and communications. The system, in its proposed form, is suitable for individual diagnostics, but is amenable to concurrent processing of large numbers of samples. The Penn team will develop the assays: reverse transcription isothermal enzymatic amplification (RT-LAMP) of viral nucleic acids with visual detection of amplicons and an immunoassay to monitor separately antibodies interactions with ZIKV and dengue virus (DENV) antigens to improve assay specificity; and the necessary hardware. ICGES will verify system's performance and ease of use with samples of ZIKV patients in Panama.

抽象的 Zika病毒（ZIKV）感染对胎儿神经发育的毁灭性影响已经是一种南美洲和中美洲的主要健康问题，可能会影响美国的部分地区。可靠的 ZIKV的诊断工具至关重要，因为受ZIKV感染的个体没有症状或非特异性表现类似于其他更常见的病毒感染的症状。这些诊断工具对于怀孕期间的风险管理；评估疫苗和疗法的功效；跟踪传播感染，跟踪ZIKV对胎儿发育的影响；协助根除工作；并确保血液供应的安全。目前，根据疾病预防控制中心，没有可靠的ZIKV诊断工具，并且管道技术主要集中在适合集中实验室的方法上，不合适资源不佳的设置和护理点。宾夕法尼亚大学之间的合作努力巴拿马的Conmemorativo de la salud（ICGES）和开发一个杂种，廉价的护理系统，用于ZIKV的分子检测和口服样品中针对ZIKV的抗体。拟议的系统可用于诊所，医生和牙医办公室，在家里，仪器最少，并且（可选）没有电力。减少成本，启用量化，系统使用智能手机进行信号检测，处理和通讯。该系统以其拟议的形式适用于个人诊断，但很适合同时处理大量样品。宾夕法尼亚团队将开发分析：反向病毒核酸的转录等温酶扩增（RT-LAMP），可视检测扩增子和免疫测定法，以分别监测与ZIKV和登革热病毒的抗体相互作用（DENV）抗原提高测定特异性；和必要的硬件。 ICGE将验证系统的巴拿马ZIKV患者样品的表现和易用性。