AB Sciex 6500+ QTRAP with Selexion for ultra-sensitive metabolomics and lipidomics

带 Selexion 的 AB Sciex 6500 QTRAP 用于超灵敏代谢组学和脂质组学

• 批准号: 9864491
• 负责人: Irwin Jack Kurland
• 金额: --
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-04-15 至 2019-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9864491
• 关键词: AB; Sciex; 6500+; QTRAP; Selexion; AB; Sciex; 6500+; QTRAP; Selexion

Abstract The ultrasensitive, Sciex 6500 hybrid triple quadrapole QTRAP with Selexion Differential Mobility Separation (DMS) and Gerstel MultiPurpose Sampler capable of automated sample derivatization sample will enable the expansion of the Mass spectrometric platform of assay modules that the Einstein Stable Isotope and Metabolomics Core (SIMC) Facility offers to researchers in the greater NY area. This hybrid mass spectrometer (MS) has unique features in comparison to conventional triple quadrapole MS. The multiple reaction monitoring (MRM) can trigger an Enhanced Product Ion scan when threshold is reached, which can confirm identity. The Selexion Differential Mobility Separation used in conjunction with UPLC for orthogonal separations, will increase S/N and enable high resolution without the need for perfect chromatography. In particular, the Selexion DMS enables resolution of structurally similar lipid species (same MRM transition) into isobaric components without the need for chiral chromatography. The Gerstel MPS will be configured for automated sample derivatization, which will be first used with dansyl chloride for enhanced detection and quantification of amines.The device will be housed and maintained in the Einstein SIMC Facility, a state of the art multiuser core facility. The SIMC is one of the premier shared facilities at Einstein. It has been very successful in giving scientists at Einstein access to the suite of mass spectrometric tools and methodologies necessary for performing metabolomic and lipidomic analyses. Currently SIMC's is limited in providing accurate quantitative metabolite and lipid profiling from small amounts of mouse or patient samples for personalized medicine initiatives. A patient biopsy, or biofluid may be needed for several cores to develop systems biology pictures of disease. Gaining access to an ultrasensitive triple quadrapole mass spectrometer will enable researchers here at Einstein to advance their research programs in a variety of fields. The Einstein Stem Cell and Diabetes Centers will utilize the increased Sciex 6500 sensitivity to examine metabolic profiles of different stem cell lineages and neural populations regulating insulin sensitivity and feeding behavior. Diabetes researchers additionally will be able to metabolically profile a single mouse hypothalamus or a small number of pancreatic islet cells, enabling new discoveries into appetite and insulin sensitivity and secretion. The Einstein Cancer, Diabetes, and Medical Counter-Measures Against Radiation Centers will utilize the increased Sciex 6500 sensitivity to examine intestinal organoid models for dietary influences on cancer development, and for assessing radiation induced gastrointestinal syndrome. As such, there is a substantial large base of pre-clinical and clinical investigators whose research will be greatly expanded by access to this instrumentation.

抽象的 超敏感的Sciex 6500混合三型四杆QTRAP与SELEXION差异迁移率分离 （DMS）和Gerstel多功能采样器能够自动样品衍生化样本将使 爱因斯坦稳定同位素和 代谢组学核心（SIMC）设施为大纽约地区的研究人员提供。这个杂种质量 与传统的三倍四极MS相比，光谱仪（MS）具有独特的特征。倍数 当达到阈值时，反应监测（MRM）可以触发增强的产品离子扫描，可以 确认身份。与UPLC一起用于正交的SELEXION差异移动性分离 分离，将增加S/N并实现高分辨率，而无需完美的色谱。在 特别是，SELEXION DMS可以解决结构相似的脂质物种（相同的MRM过渡） 同质组件无需手性色谱。 Gerstel MPS将为 自动样品衍生化，将首先与氯化丹苯基一起用于增强检测和 胺的定量。该设备将在爱因斯坦SIMC设施中容纳并维护 Art Multiuser核心设施。 SIMC是爱因斯坦的主要共享设施之一。一直非常 成功地让爱因斯坦的科学家访问质谱工具和方法的套件 进行代谢组学和脂肪组分析所需的必要。目前SIMC的提供 从少量的小鼠或患者样品中的准确定量代谢物和脂质分析 个性化医学计划。可能需要进行多个核心的患者活检或生物流体 系统生物学疾病图片。获得超敏感的三倍四极质谱仪 将使爱因斯坦的研究人员能够在各个领域推进其研究计划。爱因斯坦 干细胞和糖尿病中心将利用增加的SCIEX 6500灵敏度检查代谢谱 不同的干细胞谱系和神经种群调节胰岛素敏感性和喂养行为。 糖尿病研究人员还能够代谢单一小鼠下丘脑或小 胰岛细胞的数量，使新发现成为食欲和胰岛素敏感性和分泌。 爱因斯坦癌症，糖尿病和针对辐射中心的医学反措施将利用 提高SCIEX 6500敏感性，以检查肠癌模型对饮食的影响 发育，以及评估辐射诱导的胃肠道综合征。因此，有一个很大的 临床前和临床研究人员的大量基础将通过访问这一研究大大扩展其研究 仪器。