Ultraviolet light sensing by cryptochrome

隐花色素紫外光传感

• 批准号: 8705550
• 负责人: Todd C Holmes
• 金额: $ 20.85万
• 依托单位: UNIVERSITY OF CALIFORNIA-IRVINE
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-08-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8705550
• 关键词: Accounting; Action Potentials; Agriculture; Aloral; Animal Model; Animals; Anopheles gambiae; Arousal; Behavior; Behavior Control; Behavioral; Binding; Biological; Biological Assay; Bite; Brain; Butterflies; Centers for Disease Control and Prevention (U.S.); Chagas Disease; Circadian Rhythms; Coupling; Crying; Culicidae; Data; Dengue; Detection; Development; Devices; Disease; Disease Vectors; Drosophila genus; Drosophila melanogaster; Environmental Impact; Epidemic; Eye; Flavins; Flying body movement; Frequencies; Future; Genetic; Health; Human; Insect Control; Insect Vectors; Insecta; Laboratories; Lateral; Lead; Length; Light; Longevity; Malaria; Measures; Mediating; Medical; Membrane; Membrane Potentials; Molecular; Neurons; Opsin; Oxidation-Reduction; Performance; Photoreceptors; Phototransduction; Physiological; Physiology; Probability; Process; Property; Proteins; Relative (related person); Research; Solid; Source; Spectrum Analysis; Technology; Testing; Texas; Time; Typhus; Ultraviolet Rays; West Nile virus; Yellow Fever; base; chromophore; cost; cryptochrome; design; fly; human disease; improved; innovation; insect disease; interest; killings; millisecond; mutant; neural circuit; novel; pesticide poisoning; photoactivation; public health relevance; research study; response; sensor; two-photon; ultraviolet; vector; vector mosquito; voltage

DESCRIPTION (provided by applicant): Ultraviolet (UV) light attracts most flying insects, including human disease vectors such as mosquitoes, flies, and biting gnats; and major agricultural pests. The diseases spread by harmful insects afflict hundreds of millions people worldwide and some insect-mediated diseases such as West Nile virus and dengue fever are alarmingly on the rise in the U.S (note the current epidemic of West Nile disease which is occurring throughout the U.S. and is most severe in Texas). Medical and agricultural damage caused by UV-sensitive insects costs many billions of dollars per year, which has led to the wide use of UV lights for insect control. The currently used versions of light traps are the descendants of designs originating from the Centers for Disease Control in the 1960s. The design of insect control lights is based on the longstanding assumption that UV light detection and behavioral responses are mediated exclusively by UV-sensitive opsins in their eyes and external photoreceptors. Our laboratory has recently identified Cryptochrome (CRY) as another insect UV-sensitive photopigment as a major component for controlling fly behavioral responses to UV light. This exciting new finding followed directly from our recent discovery that blue light photoactivation of insect CRY causes rapid membrane depolarization and up to 300% increased action potential firing rate over baseline dark firing rate in central brain arousal neurons (Sheeb et al., 2007; Fogle et al., 2011). The CRY-mediated electrophysiological light response is robust in the absence of all opsin-based classical photoreceptor inputs (Fogle et al., 2011). Thus it is likely that insect control light technologies could be improved by a better understanding of the physiology of insect UV light response, including taking CRY's properties and physiologically driven processes into account. This proposal provides an innovative plan to explore UV light activation of insect CRYs including a rapid assay to assess the UV sensitivity of CRYs from all known sequences for the most harmful insect disease vectors, including the mosquito species responsible for malaria, dengue fever, yellow fever, West Nile virus and others. Other CRY sequences will become available in the near future for testing the insect vectors for Chagas disease and typhus. We had a solid plan to mechanistically determine the molecular and circuit physiology of how UV light activated CRY determines insect behavioral responses to UV light including aims that if successful will provide clear guidance for improving light devices to attrac and kill greater numbers of harmful insects. These plans include a test whether blue light pre-activation amplifies the biological response of CRY to UV light. Most of our plan centers around the use of LEDs as UV light sources. This was done in consideration for eventual field applications using LEDs for harmful insect control due to recent improvements in LED device longevity, precision of temporal control and power efficiency and low cost. The research plan is supported by very strong preliminary data for all aims and thus has a high probability for producing novel high impact findings.

描述（由申请人提供）：紫外线 (UV) 光会吸引大多数飞行昆虫，包括人类疾病媒介，如蚊子、苍蝇和叮咬昆虫；和主要农业害虫。由有害昆虫传播的疾病困扰着全世界数亿人，一些昆虫介导的疾病，如西尼罗河病毒和登革热，在美国呈惊人的上升趋势（注意目前正在美国各地流行的西尼罗河病）德克萨斯州最为严重）。对紫外线敏感的昆虫造成的医疗和农业损失每年造成数十亿美元的损失，这导致广泛使用紫外线来控制昆虫。当前使用的光陷阱版本是其后代 源自 20 世纪 60 年代疾病控制中心的设计。昆虫控制灯的设计基于长期以来的假设，即紫外线检测和行为反应完全由眼睛和外部光感受器中的紫外线敏感视蛋白介导。我们的实验室最近发现隐花色素（CRY）是另一种昆虫紫外线敏感感光色素，是控制苍蝇对紫外线行为反应的主要成分。这一令人兴奋的新发现直接源于我们最近的发现，即昆虫 CRY 的蓝光光激活会导致膜快速去极化，并且与中枢大脑唤醒神经元的基线暗放电率相比，动作电位放电率增加高达 300%（Sheeb 等，2007；Fogle）等人，2011）。在没有所有基于视蛋白的经典光感受器输入的情况下，CRY 介导的电生理光反应是稳健的（Fogle 等人，2011）。因此，通过更好地了解昆虫紫外线反应的生理学，包括考虑 CRY 的特性和生理驱动过程，昆虫控制光技术可能会得到改进。该提案提供了一项创新计划，探索昆虫 CRY 的紫外线激活，包括快速测定，以评估来自所有已知序列的 CRY 对最有害昆虫疾病载体（包括导致疟疾、登革热、黄热病的蚊子种类）的紫外线敏感性。 、西尼罗河病毒等。其他 CRY 序列将在不久的将来用于测试恰加斯病和斑疹伤寒的昆虫载体。我们有一个可靠的计划，从机械上确定紫外线激活的 CRY 如何确定昆虫对紫外线的行为反应的分子和电路生理学，包括目标，如果成功，将为改进光装置以吸引和杀死更多有害昆虫提供明确的指导。这些计划包括测试蓝光预激活是否会放大 CRY 对紫外线的生物反应。我们的大部分计划都围绕使用 LED 作为紫外线光源。这样做是为了考虑到最终使用 LED 进行有害昆虫控制的现场应用，因为最近在 LED 器件寿命、时间控制精度以及功率效率和低成本方面取得了进步。该研究计划的所有目标都得到了非常有力的初步数据的支持，因此很有可能产生新颖的高影响力的发现。