Tissue Oxygen (pO2) Measurement by Photoacoustic Imaging

通过光声成像测量组织氧 (pO2)

基本信息

批准号：
8338824
负责人：
Shai Ashkenazi
金额：
$ 15.91万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-09-26 至 2013-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8338824
关键词：
Address Affect Algorithms Animal Experiments Animals Bladder Blood Vessels Cancer Biology Cancer Patient Cell Cycle Arrest Cell Death Cells Clinical Decision Making Development Devices Diagnosis Dyes Electrodes Elements Endoscopes Esophagus Fluorescence Foundations Functional Imaging Gold Head and Neck Cancer Hypoxia Image Imaging Device Imaging Phantoms Imaging technology In Vitro Large Intestine Larynx Lead Light Lighting Location Malignant Neoplasms Maps Masks Measurement Measures Mechanics Medical Methods Monitor Oncologist Optics Oral cavity Outcome Oxygen Oxygen Consumption Penetration Phase Photochemotherapy Photosensitizing Agents Physicians Play Pump Radiation Oncology Radiation therapy Reaction Reactive Oxygen Species Research Research Proposals Resistance Resolution Role Scanning Skin Cancer Software Design Solid Stomach System Techniques Technology Testing Time Tissue Sample Tissues Transducers Translating Tumor Oxygenation Ultrasonography Vision Work absorption base cancer cell cancer diagnosis cancer therapy cancer type chemotherapy clinical practice imaging modality improved malignant breast neoplasm minimally invasive new growth new technology oncology optical fiber outcome forecast pre-clinical preclinical study prototype research clinical testing research study tissue oxygenation tool treatment planning tumor tumor progression

项目摘要

DESCRIPTION (provided by applicant): Tumor hypoxia is observed in many cancer types. It is believed to be a consequence of unorganized growth of new vasculature. Cancer cells close to the blood vessels have high oxygen consumption due to rapid proliferation. Cells that are farther away are masked from oxygen supply. These hypoxic cancer cells are mostly in a phase of cell cycle arrest and therefore become highly resistant to chemotherapy and radiation therapy which mostly affects dividing cells. Different therapy techniques have been studied to target hypoxic tumors. To predict the efficacy of radiation therapy for a cancer patient and to select optimal therapy strategy it is essential to assess oxygen distribution in the tissue. However, none of the existing methods for assessing tissue oxygenation has yet been established in the clinical arena. In this R21 application we propose to develop a non-invasive, high resolution imaging method for tissue oxygen. Based on our recent development of photoacoustic probing of oxygen sensitive dye's lifetime, we propose a technique that combines the accuracy and sensitivity of time-resolved fluorescence methods with the high resolution and deep penetration of photoacoustic imaging. It has the potential to fill the gap for a fast, easy to use, real-time imaging of tumor hypoxia. This research proposal addresses the basic aspects of developing this new imaging method. First, we will build a prototype system that will be tested on phantoms. This would be our prime vehicle for optimizing the system hardware design and software algorithms. Then we will test the system and compare different dye administration methods on small animals. These results will serve as a basis for a continuing research to translate it into a clinical tool. Our vision for the ultimate outcome of this project is a non-invasive clinical imaging modality for better prognosis and treatment decision making in most cases of head and neck cancers (where tumor is accessible to light penetration). For deeper tumor locations (e.g. breast cancer tumors) invasive optical fiber illumination will be considered. Such imaging modality could have a profound impact on clinical practice in oncology and particularly in radiation oncology.

描述（由申请人提供）：在许多癌症类型中观察到肿瘤缺氧。据信这是新脉管系统无组织生长的结果。靠近血管的癌细胞由于增殖速度快，耗氧量高。距离较远的细胞无法获得氧气供应。这些缺氧的癌细胞大多处于细胞周期停滞阶段，因此对化疗和放疗具有高度抵抗力，而化疗和放疗主要影响分裂细胞。已经研究了不同的治疗技术来针对缺氧肿瘤。为了预测癌症患者放射治疗的疗效并选择最佳治疗策略，评估组织中的氧分布至关重要。然而，临床领域尚未建立评估组织氧合的现有方法。在这个 R21 应用中，我们建议开发一种非侵入性、高分辨率的组织氧成像方法。基于我们最近对氧敏感染料寿命的光声探测的发展，我们提出了一种将时间分辨荧光方法的准确性和灵敏度与光声成像的高分辨率和深度穿透相结合的技术。它有潜力填补肿瘤缺氧快速、易于使用、实时成像的空白。该研究提案解决了开发这种新成像方法的基本方面。首先，我们将构建一个原型系统，并在模型上进行测试。这将是我们优化系统硬件设计和软件算法的主要工具。然后我们将测试该系统并在小动物上比较不同的染料施用方法。这些结果将作为持续研究的基础，将其转化为临床工具。我们对该项目最终结果的愿景是一种非侵入性临床成像方式，可以在大多数头颈癌（肿瘤可被光穿透）的情况下提供更好的预后和治疗决策。对于较深的肿瘤位置（例如乳腺癌肿瘤），将考虑侵入性光纤照明。这种成像方式可能会对肿瘤学，特别是放射肿瘤学的临床实践产生深远的影响。