A noninvasive method for tissue stiffness quantification in small animals with shear wave elastography

一种利用剪切波弹性成像对小动物组织硬度进行无创定量的方法

• 批准号: 9516302
• 负责人: Tomasz Joseph Czernuszewicz
• 金额: $ 3万
• 依托单位: SONOVOL, INC.
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-05 至 2018-03-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9516302
• 关键词: 3D ultrasound; Abdomen; Acoustics; Address; Adopted; Adoption; Affect; Americas; Anatomy; Animal Experimentation; Animal Model; Animals; Autopsy; Biological; Biological Models; Biological Sciences; Breast; Calibration; Cardiac; Clinic; Clinical; Clinical Trials; Communities; Community Developments; Data; Data Collection; Devices; Diagnosis; Disease; Disease model; Elasticity; Ensure; Environment; FDA approved; Fibrosis; Frequencies; Funding; Future; Geometry; Gold; Hand; Heating; Histologic; Human; Image; Imaging Device; Imaging Phantoms; Imaging Techniques; Imaging technology; In Vitro; Knowledge; Laboratories; Laboratory Research; Liver; Liver Cirrhosis; Liver Fibrosis; Liver diseases; Longitudinal Studies; Malignant Neoplasms; Manuals; Measurement; Measures; Mechanics; Methods; Modeling; Monitor; Mus; Organ; Outcome; Output; Pathology; Patients; Penetration; Pharmaceutical Preparations; Phase; Physiologic pulse; Positioning Attribute; Prostate; Protocols documentation; Publishing; Radiology Specialty; Reproducibility; Research; Research Personnel; Resolution; Robotics; Rodent; Rodent Model; Scanning; Sensitivity and Specificity; Skin; Software Tools; Speed; Staining method; Stains; Surface; System; Techniques; Technology; Temperature; Testing; Thyroid Gland; Time; Tissues; Training; Transducers; Travel; Ultrasonics; Ultrasonography; Variant; Work; animal imaging; base; cancer imaging; choline deficient diet; cohort; cost; design; drug development; elastography; imaging platform; imaging system; improved; in vivo; innovation; interest; killings; liver biopsy; liver development; mouse model; novel; pre-clinical; prevent; research and development; response; robot control; skills; success; tool

Abstract Liver fibrosis, a hallmark of many liver diseases, affects millions of people in America every year, and annually kills tens of thousands of these patients. Despite very active research efforts, there still have been no specific antifibrotic drugs approved by the FDA. The liver disease and drug development communities do not currently have well-validated, low-cost, easy to use, noninvasive tools for studying liver fibrosis in preclinical rodent models making it challenging to execute high-quality longitudinal studies. To address this need, SonoVol Inc. proposes to develop a novel benchtop imaging system capable of providing rapid, noninvasive measurements of liver fibrosis in rodents with robotically-controlled shear wave elasticity imaging (SWEI). In SWEI, ultrasound pulses are used to generate shear waves in an organ an interest, and tissue stiffness can be inferred by monitoring the speed of the travelling waves. SWEI technology has been very successful in clinical trials and is starting to be adopted by abdominal radiology clinics around the world as a replacement for liver biopsy. While some preclinical SWEI products exist on the market today, all of them follow the conventional ultrasound paradigm of data collection – a trained sonographer uses a handheld probe and manually selects regions of interest to interrogate with SWEI. Making consistent and accurate measurements requires in depth knowledge of probe placement and sonographic technique, which academic biologists or drug researchers are unlikely to possess. Therefore, SonoVol will develop a device capable of fully-automated, non-contact SWEI that eliminates the need for a trained sonographer and will enable large scale adoption of this powerful technology in the preclinical liver diseases research community. To validate the new system, two different animal models for hepatic fibrosis will be evaluated, and the results compared to conventional postmortem assessments of liver fibrosis. This technology represents an innovative combination of a widefield 3D robotic ultrasound imaging system and noninvasive shear wave elastography. Furthermore, the technology can be applied in the future to many other diseases, including cancer or cardiac models, increasing the potential market and impact on the field.

抽象的 肝纤维化是许多肝病的标志，影响着美国数百万的人 一年，每年杀死成千上万的患者。尽管研究工作非常积极， FDA批准的仍然没有特定的抗纤维化药物。肝病和 药物开发社区目前尚无验证，低成本，易于使用， 临床前啮齿动物模型中研究肝纤维化的无创工具，使其挑战 执行高质量的纵向研究。 为了满足这一需求，Sonovol Inc.提议开发新型的台式成像系统 能够在啮齿动物中提供快速的无创测量 机器人控制的剪切波弹性成像（SWEI）。在Swei中，超声脉冲是 用于在器官中产生剪切波的一种有趣的剪切波，可以通过 监视行进波的速度。 Swei技术在 临床试验，并开始被世界各地的腹部放射学诊所采用 替代肝活检。尽管当今市场上存在一些临床前SWEI产品，但 所有人都遵循传统的数据收集超声范式 - 经过训练的 Sonographer使用手持式探测器，并手动选择感兴趣的区域来询问 swei。进行一致，准确的测量需要深入了解证明 学术研究人员或药物研究人员的安置和声学技术 不太可能拥有。 因此，Sonovol将开发一种能够完全自动化的，非接触性Swei的设备 消除了对经过训练的超声师的需求，并将大规模采用这一要求 临床前肝脏疾病研究界的强大技术。验证新的 系统，将评估两种不同的用于肝纤维化的动物模型，结果 与肝纤维化的常规死后评估相比。该技术代表 广场3D机器人超声成像系统和无创的创新组合 剪切波弹性图。此外，该技术将来可以应用于许多 其他疾病，包括癌症或心脏模型，增加了潜在市场和影响 在场上。