Protein Quality Early in Life: Mechanisms of Growth and Later Obesity Development

生命早期的蛋白质质量：生长和后期肥胖发展的机制

• 批准号: 9352844
• 负责人: Minghua Tang
• 金额: $ 13.4万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-14 至 2020-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9352844
• 关键词: ATP phosphohydrolase; Achievement; Affect; Age-Months; Amino Acids; Arginine; Bacteroidetes; Biological Markers; Biometry; Birth; Body Composition; Body fat; Branched-Chain Amino Acids; Child Nutrition; Clinical Skills; Colorado; Complementary Feeding; Data; Data Analyses; Development; Diet; Dietary Proteins; Domestic Fowls; Early Intervention; Environment; Epidemiology; Exposure to; Faculty; Fatty acid glycerol esters; Fermentation; Fishes; Food; Future; Genes; Glycoside Hydrolases; Goals; Growth; Harvest; Homeostasis; Impairment; Individual; Infant; Institution; Insulin Resistance; Insulin-Like Growth Factor I; Investigation; Iron; Knowledge; Leadership; Life; Literature; Long-Term Effects; Marketing; Meat; Mediator of activation protein; Medical; Mentors; Metagenomics; Microbe; Micronutrients; Mission; Monitor; Obesity; Outcome; Overweight; Participant; Pattern; Play; Positioning Attribute; Postdoctoral Fellow; Prevention; Proteins; Protocols documentation; Public Health; Randomized Controlled Trials; Recommendation; Regulation; Research; Research Methodology; Research Training; Ribosomal RNA; Risk; Risk Factors; Role; Sampling; Serum; Solid; Source; Statistical Methods; Taxonomy; Techniques; Time; Toddler; Training; Training Programs; United States National Institutes of Health; Universities; Weight; Weight Gain; Zinc; acylcarnitine; base; career; career development; cohort; doubly-labeled water; effective intervention; evidence base; experience; feeding; gut microbiome; heart disease risk; human disease; insulin sensitivity; meetings; metabolomics; metagenomic sequencing; microbiome; modifiable risk; multidisciplinary; nutrition; obesity in children; obesity prevention; obesogenic; prevent; programs; protein intake; randomized trial; research and development; skills; sound; total energy expenditure; trimethyloxamine

ABSTRACT Rapid weight gain during the first two years of life predisposes to later-in-life obesity. However, there is a fundamental gap in knowledge of what dietary recommendations should be provided to infants and toddlers to promote optimal growth and prevent rapid weight gain. Dietary protein intake is considered a risk factor because it accelerates weight and fat gain in infants and toddlers, based primarily on research of dairy protein. However, the underlying mechanisms are not clear. These findings have been the basis of recent recommendations to reduce protein intake from birth to 24 months. What is not known is whether all protein sources have the same weight-accelerating effect. Our research group conducted a randomized controlled trial in infants consuming a meat- or dairy-based complementary diet from 6 to 12 months of age. Growth data showed that a dairy-based complementary diet, not meat, leads to impaired linear growth and increased risk for being overweight. The objective of this proposed project is to investigate the underlying mechanisms and determine which mediators are predictive for the different growth patterns in this cohort via fecal microbiome/metagenomics, serum metabolomics and doubly labeled water technique. Although the candidate has expertise in pediatric nutrition and conducting randomized controlled trials, training in mechanistic investigations of obesity development and research methodologies are critical to achieve the long-term goal of becoming a leader in pediatric nutrition focusing on implementing effective interventions early in life for optimal growth and obesity prevention. This career development application proposes a 4-year multidisciplinary training program to the candidate, who is a 4th year postdoctoral fellow transitioning to a junior faculty position. A well-developed training plan by the candidate and the mentoring team is in place, including 1) Hands-on experience of microbiome/metagenomics, metabolomics analysis and data interpretation; 2) Hands-on experience of conducting the doubly labeled water protocol for body composition and total energy expenditure in infants and toddlers, plus sample analysis and data interpretation; 3) Regular meetings with mentors to monitor project progress, achievements towards the short- and long-term goals, and didactic trainings; 4) Advanced coursework in microbiome, metabolomics and biostatistics; 5) Training in leadership and program management. University of Colorado Anschutz Medical Campus is world-leading research institution and provides the candidate an excellent research and training environment which facilitates a successful transition to an independent career in pediatric nutrition and early-in-life obesity prevention.

抽象的 在生命的头两年中，体重快速增加，易于肥胖。但是，有一个 了解应向婴儿和幼儿提供哪些饮食建议的基本差距 促进最佳生长并防止体重快速增加。饮食蛋白摄入被认为是危险因素 因为它主要基于对乳制品蛋白的研究加速婴儿和幼儿的体重和脂肪增加。 但是，基本机制尚不清楚。这些发现是最近的基础 从出生到24个月的蛋白质摄入量的建议。尚不清楚的是是否所有蛋白质 来源具有相同的权重加速效果。我们的研究小组进行了随机控制 在6到12个月大的婴儿中，婴儿的饮食习惯了肉类或乳制品的互补饮食。生长 数据表明，基于乳制的互补饮食而不是肉会导致线性生长受损并增加 超重的风险。该拟议项目的目的是调查潜在机制和 确定哪些介体通过粪便可以预测该队列中不同的生长模式 微生物组/宏基因组学，血清代谢组学和双重标记的水技术。虽然 候选人在小儿营养和进行随机对照试验，培训方面具有专业知识 肥胖发展和研究方法的机械研究对于实现 成为小儿营养领域的领导者的长期目标，专注于实施有效的干预措施 生命的早期，以预防最佳生长和肥胖。 该职业发展申请为候选人提出了为期4年的多学科培训计划， 他是博士后四年级，过渡到初级教师职位。一个发达的培训计划 由候选人和指导团队到位，包括1） 微生物组/宏基因组学，代谢组学分析和数据解释； 2）实践经验 在婴儿中进行双重标记的水协议，以用于婴儿的身体成分和总能量消耗 和幼儿，以及样本分析和数据解释； 3）与导师的定期会议以监视 项目进步，对短期和长期目标的成就以及教学培训； 4）高级 微生物组，代谢组学和生物统计学的课程； 5）领导力和计划的培训 管理。科罗拉多大学安索兹大学医学校园是世界领先的研究机构， 为候选人提供了一个出色的研究和培训环境，促进成功 过渡到小儿营养和早期肥胖症预防的独立职业。