The ocular surface microbiome in potentially infectious ophthalmic disease

潜在传染性眼科疾病中的眼表微生物组

• 批准号: 9185320
• 负责人: Cecilia Sungmin Lee
• 金额: $ 19.91万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-01 至 2019-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9185320
• 关键词: Aftercare; Archives; Artificial Tears; Biopsy; Blindness; Characteristics; Clinical; Clinical Research; Clinical Trials; Communicable Diseases; Communities; Complex; Computer Simulation; Conjunctivitis; Cyclosporine; DNA; DNA sequencing; Detection; Development; Disease; Disease Outcome; Dry Eye Syndromes; Endophthalmitis; Epidemic Keratoconjunctivitis; Epidemiology; Eye; Eye Infections; Eye diseases; Goals; Health; Infection; Institution; K-Series Research Career Programs; Karyotype determination procedure; Keratitis; Lead; Master of Science; Measures; Mentors; Mentorship; Methods; Microbiology; Morbidity - disease rate; Ophthalmology; Organism; Outcome; Pathogenesis; Patients; Physicians; Physiological; Randomized; Randomized Clinical Trials; Research; Research Personnel; Resolution; Retrospective Studies; Role; Sampling; Scientist; Severity of illness; Signs and Symptoms; Structure; Swab; Techniques; Training; Transfusion-Transmitted Virus; Treatment outcome; Viral; Virus; Visual; Visual Acuity; arm; base; career; cohort; experience; microbial; microbiome; molecular diagnostics; novel; ocular surface; pathogen; prospective; public health relevance; treatment group; treatment response

 DESCRIPTION (provided by applicant): Ocular infectious diseases such as endophthalmitis and conjunctivitis remain a significant cause of visual morbidity and potential blindness. However, traditional microbial cultures often fail to identify causative organisms in a high proportion of clinically suspicious infections. Most pathogens responsible for ocular infections originate from the ocular surface. Thus, characterizing the ocular surface microbiome in different disease states may lead to a better understanding of the pathogenesis of these conditions. Our goal is to understand the community structure of ocular surface microbial flora that predisposes the eye to infection and influences various disease courses. Our overarching hypothesis is that the constituents of the ocular surface microbiome are involved in the pathogenesis of potentially infectious ophthalmic conditions. To explore this hypothesis, we propose to study the ocular surface microbiome, in particular a recently described virus, torque teno virus (TTV), in three diseases by using a novel, deep DNA sequencing technique. In specific aim 1, we propose one prospective and one retrospective study to determine the correlation between the ocular surface microbiome and clinical outcomes of epidemic keratoconjunctivitis and endophthalmitis. In specific aim 2, we propose a randomized clinical trial to study the ocular surface microbiome and its relationship to the treatment outcome in patients with dry eye syndrome. My ultimate career goal is to become an independent physician-scientist in the field of ophthalmology. I am committed to developing a clinical research-oriented career in major academic institutions and understand the need for me to pursue additional training and mentorship. During the Mentored Research Career Development Award period, I plan to obtain a Master of Science in Epidemiology (Clinical Research Track) and participate in several, focused didactic coursework in microbiology, fundamentals of clinical research, and molecular diagnostics. The additional training, research experience, and most importantly, mentorship by committed experts will be of uttermost importance in becoming a successful clinician-researcher.

 描述（由适用提供）：眼部传染病，例如内嗜性疾病和结膜炎，仍然是视觉发病率和潜在失明的重要原因。但是，传统的微生物培养物通常无法以很大比例的临床可疑感染鉴定灾难性生物。大多数导致眼部感染的病原体源自眼表面。在不同疾病状态下的眼部表面微生物组表征这可能会导致对这些疾病发病机理的更好理解。我们的目标是了解眼部微生物菌群的社区结构，使眼睛容易感染并影响各种疾病课程。我们的总体假设是，眼表微生物组的构成参与潜在感染性眼科条件的发病机理。为了探讨这一假设，我们建议通过使用一种新颖的深层DNA测序技术研究眼表面微生物组，特别是最近描述的病毒扭矩Teno病毒（TTV）。在特定的目标1中，我们提出了一项前瞻性和一项回顾性研究，以确定眼部表面微生物组与流行角膜结膜炎和内咽炎的临床结局之间的相关性。在特定的目标2中，我们提出了一项随机临床试验，以研究眼部表面微生物组及其与干眼综合征患者的治疗结果的关系。我的最终职业目标是成为眼科领域的独立身体科学家。我致力于在主要学术机构中发展面向临床研究的职业，并了解我需要进行额外的培训和心态的需求。在指导的研究职业发展奖期间，我计划获得流行病学科学硕士学位（临床研究轨道），并参加了几个以微生物学，临床研究基础和分子诊断的重点教学课程。额外的培训，研究经验以及最重要的是，坚定的专家的精神训练对于成为一名成功的临床研究者至关重要。