The ocular surface microbiome in potentially infectious ophthalmic disease

潜在传染性眼科疾病中的眼表微生物组

基本信息

批准号：
9185320
负责人：
Cecilia Sungmin Lee
金额：
$ 19.91万
依托单位：
UNIVERSITY OF WASHINGTON
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-12-01 至 2019-11-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9185320
关键词：
Aftercare Archives Artificial Tears Biopsy Blindness Characteristics Clinical Clinical Research Clinical Trials Communicable Diseases Communities Complex Computer Simulation Conjunctivitis Cyclosporine DNA DNA sequencing Detection Development Disease Disease Outcome Dry Eye Syndromes Endophthalmitis Epidemic Keratoconjunctivitis Epidemiology Eye Eye Infections Eye diseases Goals Health Infection Institution K-Series Research Career Programs Karyotype determination procedure Keratitis Lead Master of Science Measures Mentors Mentorship Methods Microbiology Morbidity - disease rate Ophthalmology Organism Outcome Pathogenesis Patients Physicians Physiological Randomized Randomized Clinical Trials Research Research Personnel Resolution Retrospective Studies Role Sampling Scientist Severity of illness Signs and Symptoms Structure Swab Techniques Training Transfusion-Transmitted Virus Treatment outcome Viral Virus Visual Visual Acuity arm base career cohort experience microbial microbiome molecular diagnostics novel ocular surface pathogen prospective public health relevance treatment group treatment response

项目摘要

DESCRIPTION (provided by applicant): Ocular infectious diseases such as endophthalmitis and conjunctivitis remain a significant cause of visual morbidity and potential blindness. However, traditional microbial cultures often fail to identify causative organisms in a high proportion of clinically suspicious infections. Most pathogens responsible for ocular infections originate from the ocular surface. Thus, characterizing the ocular surface microbiome in different disease states may lead to a better understanding of the pathogenesis of these conditions. Our goal is to understand the community structure of ocular surface microbial flora that predisposes the eye to infection and influences various disease courses. Our overarching hypothesis is that the constituents of the ocular surface microbiome are involved in the pathogenesis of potentially infectious ophthalmic conditions. To explore this hypothesis, we propose to study the ocular surface microbiome, in particular a recently described virus, torque teno virus (TTV), in three diseases by using a novel, deep DNA sequencing technique. In specific aim 1, we propose one prospective and one retrospective study to determine the correlation between the ocular surface microbiome and clinical outcomes of epidemic keratoconjunctivitis and endophthalmitis. In specific aim 2, we propose a randomized clinical trial to study the ocular surface microbiome and its relationship to the treatment outcome in patients with dry eye syndrome. My ultimate career goal is to become an independent physician-scientist in the field of ophthalmology. I am committed to developing a clinical research-oriented career in major academic institutions and understand the need for me to pursue additional training and mentorship. During the Mentored Research Career Development Award period, I plan to obtain a Master of Science in Epidemiology (Clinical Research Track) and participate in several, focused didactic coursework in microbiology, fundamentals of clinical research, and molecular diagnostics. The additional training, research experience, and most importantly, mentorship by committed experts will be of uttermost importance in becoming a successful clinician-researcher.

描述（由申请人提供）：眼内炎和结膜炎等眼部感染性疾病仍然是视觉发病和潜在失明的重要原因，然而，传统的微生物培养往往无法识别大部分临床可疑感染的致病微生物。眼部感染起源于眼表，因此，表征不同疾病状态下的眼表微生物组可能有助于更好地了解这些病症的发病机制。眼表微生物菌群的群落结构使眼睛容易受到感染并影响各种疾病进程，我们的首要假设是眼表微生物组的成分参与了潜在感染性眼科疾病的发病机制。通过使用一种新颖的深度 DNA 测序技术来研究三种疾病中的眼表微生物组，特别是最近描述的病毒，扭矩腱病毒（TTV）。在具体目标 1 中，我们提出了一个前景和一个。确定眼表微生物群与流行性角结膜炎和眼内炎临床结果之间相关性的回顾性研究在具体目标 2 中，我们提出了一项随机临床试验来研究干眼综合征患者的眼表微生物群及其与治疗结果的关系。我的最终职业目标是成为眼科领域的独立医师科学家。我致力于在主要学术机构发展以临床研究为导向的职业，并了解我需要接受额外的培训。在指导研究职业发展奖期间，我计划获得流行病学理学硕士学位（临床研究方向），并参加一些微生物学、临床研究基础知识和分子诊断方面的重点教学课程。最重要的是，忠诚的专家的指导对于成为一名成功的临床医生研究员至关重要。