Parallel Tectothalamic Pathways

平行的顶盖丘脑通路

基本信息

批准号：
9129777
负责人：
MARTHA E BICKFORD
金额：
$ 43.69万
依托单位：
UNIVERSITY OF LOUISVILLE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-09-02 至 2019-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9129777
关键词：
Address Affect Animals Back Butyric Acids Cell Nucleus Cells Code Detection Disease Dorsal Dyslexia Electron Microscopy Electrons Electrophysiology (science)Health Homologous Gene In Vitro Investigation Lateral Lateral Geniculate Body Lateral posterior nucleus of thalamus Literature Microscopic Motion Motion Perception Movement Mus Neurons Output Pathway interactions Perception Population Process Property Pulvinar structure Reaction Research Rodent Schizophrenia Signal Transduction Site Stimulus Stream Structure Synapses Techniques Tectum Mesencephali Thalamic structure Vision Visual Visual Motion Visual system structure Whole-Cell Recordings area striata base brain circuitry cell type detector extrastriate extrastriate visual cortex insight magnocellular motion sensitivity novel optogenetics parvocellular receptive field research study response segregation superior colliculus Corpora quadrigemina visual neuroscience visual stimulus

项目摘要

DESCRIPTION (provided by applicant): The superficial layers of the superior colliculus (SC) contain two cell types that both respond to the movement of visual stimuli, but are morphologically and functionally distinct. A projection from the SC to the lateral posterior nucleus (LPN) originates from wide-field vertical (WFV) cells, while a projection from the SC to the dorsal lateral geniculate nucleus (dLGN) originates from narrow-field vertical (NFV) cells. WFV cells have been described as motion detectors based on their responses to small stimuli moving in any direction within a very large receptive field. In contrast, NFV cells may be specialized to code more detailed motion parameters based on their small receptive fields and strong direction selectivity. The parallel WFV and NFV pathways remain segregated in that the tectorecipient dLGN and LPN project differentially to the striate and extrastriate cortex. However, little is known regarding the interaction between the SC, the tectorecipient thalamus, and the cortex. We propose to analyze the circuits that connect these structures in mice by using novel combinations of optogenetics, in vitro whole cell recordings from neuronal populations identified by retrograde tracing techniques, as well as quantitative electron microscopic investigation of synaptic connections. The Aim 1 experiments will use in vitro whole cell recording from identified cortical cell populations and optogenetic activation of terminals tht originate from the tectorecipient dLGN or LPN to determine which cell types are directly innervated and to characterize the electrophysiological properties of these synapses. Electron microscopy will quantify ultrastructural features of these synapses. The Aim 2 experiments will use in vitro whole cell recordings from NFV and WFV cells and optogenetic activation of corticotectal terminals to determine whether these cells receive direct or indirect input from V1 or the lateral extrastriate cortex, and to characterize the electrophysiological properties of thes connections. Electron microscopy will quantify ultrastructural features of corticotectal synapses and the distribution inputs to NFV and WFV cells that do and do not contain gamma amino butyric acid (GABA). As comparisons of parallel geniculocortical pathways have led to insights regarding cortical processing streams, a comparison of WFV and NFV tecto-thalamo-cortical pathways will help us to understand how different aspects of visual motion are utilized by the visual system. In addition, our circuit analysis can help reveal whether corticotectal pathways are organized to enhance segregation, or synthesis, of motion signals

描述（由申请人提供）：上丘丘（SC）的表面层包含两种细胞类型，这些细胞类型都响应视觉刺激的运动，但在形态学和功能上是不同的。从SC到侧后核（LPN）的投影起源于宽场垂直（WFV）细胞，而从SC到背侧侧向基因化核（DLGN）的投影来自窄场垂直（NFV）细胞。 WFV细胞已被描述为运动探测器，这些探测器基于对小刺激在非常大的接收场中任何方向移动的小刺激的响应。相比之下，NFV细胞可以专门根据其小型接受场和强大的方向选择性编码更详细的运动参数。平行的WFV和NFV途径仍然被隔离，因为TectorEcipent DLGN和LPN差异地投影到了分层和腹部外皮质。但是，关于SC，TecoreCipient丘脑和皮层之间的相互作用知之甚少。我们建议通过使用新颖的光遗传学组合，通过逆行跟踪技术鉴定出的神经元种群的体外全细胞记录以及突发连接的定量电子显微镜研究来分析小鼠连接这些结构的电路。 AIM 1实验将使用来自已鉴定的皮质细胞群体的体外全细胞记录，终端的光遗传学激活来自TectorCipient DLGN或LPN，以确定哪些细胞类型直接支配并表征这些突触的电生理特性。电子显微镜将量化这些突触的超微结构特征。 AIM 2实验将使用NFV和WFV细胞中的体外全细胞记录以及皮质直直直直截止末端的光遗传学激活，以确定这些细胞是从V1还是从V1或横向外皮层接收直接或间接输入的，并表征该连接的电生理特性。电子显微镜将量化皮质直肠突触的超微结构特征，以及对NFV和WFV细胞的分布输入，该特征是和不含γ氨基丁酸（GABA）的NFV和WFV细胞。由于对平行基质皮层途径的比较导致了有关皮质加工流的见解，因此对WFV和NFV Tecto-Tecto-thalamo-cortical途径的比较将有助于我们了解视觉系统的视觉运动的不同方面。此外，我们的电路分析可以帮助揭示皮质直直直直直直径是为了增强运动信号的分离或合成的组织