SEM for serial block-face imaging

用于连续块面成像的 SEM

基本信息

批准号：
9273668
负责人：
ROBBERT J CRETON
金额：
$ 79.06万
依托单位：
BROWN UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-04-14
项目状态：
已结题

项目摘要

Summary Brown University requests support for a FEI Teneo VS variable pressure Scanning Electron Microscope (SEM) for serial block face imaging in the life sciences. Ultrathin sections are cut inside the microscope and series of images are acquired from the freshly cut block face, while the sections are discarded. The automated system is capable of imaging large z-stacks at isotropic 10x10x10 nm resolution. The series of images can be processed for 3D reconstruction of cells and tissues, with sufficient resolution to identify small vesicles, organelles and neural connections. The user group requested the basic Teneo VS system. This system includes equipment essential for serial block-face imaging and 3D reconstruction of the data sets, but does not include additional features or accessories. The requested system will replace a 25-year old SEM in the Division of Biology and Medicine at Brown University. The Teneo VS will be the only SEM in the Division of Biology and Medicine and the only variable pressure SEM for serial block-face imaging in the State of Rhode Island. The microscope will be used immediately by 15 investigators at Brown University and Rhode Island Hospital for high-resolution 3D imaging of cellular and sub-cellular structures ranging from germ plasm in embryos to neural circuits in the retina. Collectively, these 15 users are funded by 26 NIH grants. The Major Users have a substantial need for the requested SEM for serial block-face imaging. As an indication of this substantial need, all Major Users have obtained preliminary results with various out- of-state SEMs for serial block-face imaging. While these preliminary results demonstrate the Major User group's need and expertise, the major users had limited access to the SEMs for serial block-face imaging and could only image a subset of samples that were important to their research programs. The Minor Users were unable to gain access to SEMs for serial block-face imaging, but have research projects that would immediately benefit from the proposed equipment. The impact of the proposed imaging system will extend beyond the projects of the Major and Minor Users, since the microscope will be made be made available to the broader scientific community in Rhode Island, which has more than 600 active NIH-funded projects with a total annual budget of $184 million. The microscope will be set up in the Leduc Bioimaging Facility at Brown University, which has the technical expertise to maintain the instrument, train its users and provide consultation on planned and ongoing research projects. In addition, the users will have access to assistance in sample preparation and data analysis. The Leduc Bioimaging Facility, supported by a broadly-based Advisory Committee, has an excellent track-record maintaining various high-end imaging systems and has strong institutional support, which guarantees that the instrumentation will be used to its maximum potential for years to come.

概括布朗大学的要求支持FEI TENEO与可变压力扫描电子显微镜（SEM）生命科学中的串行块面对成像。超薄部分被切开在显微镜内，并且一系列图像是从新鲜切割的块面中获取的，而部分则丢弃。这自动化系统能够在各向同性10x10x10 nm分辨率下对大型Z堆栈进行成像。系列可以处理图像以进行3D重建细胞和组织，并具有足够的分辨率来识别小囊泡，细胞器和神经连接。用户组请求基本的Teneo与系统。该系统包括串行块面成像和数据重建的设备集合，但不包括其他功能或配件。请求的系统将替换25年布朗大学生物学和医学系的旧SEM。 Teneo vs将是唯一的SEM 生物学和医学的划分以及唯一的可变压力SEM用于串行块面成像罗德岛州。显微镜将立即由布朗大学的15名调查员使用和罗德岛医院用于细胞和亚细胞结构的高分辨率3D成像从胚胎中的细菌等血浆到视网膜中的神经回路。总的来说，这15位用户由26 NIH赠款。主要用户非常需要要求的SEM进行串行块面成像。为了表明这种巨大的需求，所有主要用户都获得了初步结果用于串行块状成像的状态SEMS。这些初步结果证明了主要用户小组的需求和专业知识，主要用户可以有限地访问SEMS进行串行块面成像并且只能想象一部分对其研究计划很重要的样本。次要用户无法访问SEMS进行串行块状成像，但是有研究项目立即受益于拟议的设备。提议的成像系统的影响将扩展除了主要用户和次要用户的项目之外，将使显微镜可用罗德岛州的更广泛的科学界，该社区拥有600多个活跃的NIH资助项目年度总预算为1.84亿美元。显微镜将在Leduc生物成像设施中设置布朗大学（Brown University）具有维护乐器，培训用户并提供的技术专长就计划和正在进行的研究项目咨询。此外，用户将可以访问帮助样本准备和数据分析。由基于广泛的咨询委员会，拥有出色的轨道记录，可维护各种高端成像系统并拥有强大的机构支持，可以保证该工具将用于其未来几年的最大潜力。