High throughput laboratory X-ray illumination system for protein crystallography and screening

用于蛋白质晶体学和筛选的高通量实验室 X 射线照明系统

基本信息

批准号：
9202439
负责人：
Wenbing Yun
金额：
$ 82.53万
依托单位：
SIGRAY, INC.
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-18 至 2018-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9202439
关键词：
Active Sites Agriculture Alzheimer&apos s Disease Anodes Antibodies Area Biological Process Biological Sciences Bioremediations Budgets Cells Characteristics Chemicals Clinical Clinical Trials Collaborations Computer Simulation Coupled Crystallization Crystallography DNA Databases Deposition Development Diabetes Mellitus Diamond Drug Design Environment Enzymes Excision Food Genetic Materials HGF gene High temperature of physical object Indium Knowledge Laboratories Legal patent Letters Lighting Liquid substance Macular degeneration Malignant Neoplasms Metabolic Pathway Metals Methods Molecular Structure Neurodegenerative Disorders New York Nucleic Acids Optics Pattern Performance Pharmaceutical Preparations Pharmacology Phase Play Principal Investigator Process Production Property Protein Engineering Proteins RNA Regulatory Pathway Research Roentgen Rays Role Signal Transduction Source Speed Staging Structure Synchrotrons System Techniques Testing Therapeutic Thermal Conductivity Training Universities Viral Proteins Work X-Ray Crystallography abstracting base beamline density design drug development drug discovery high throughput screening human disease improved innovation insight interest macromolecule meetings novel operation programs protein structure prototype research study screening structural biology therapeutic target three dimensional structure tool virus genetics wasting

项目摘要

Title: High performance, high throughput laboratory x-ray illumination system for protein crystallography Project Summary and Abstract X-ray protein crystallography is the leading method for determining the 3D structures of macromolecules (proteins, viruses, nucleic acids). Structural understanding provides key insight into their functionality and is now an essential tool for industrial applications in food & agriculture, protein engineering, and drug design. This has resulted in several high profile late-stage clinical drug trials for cancer and Alzheimer’s therapeutics. We propose to develop an X-ray illumination system comprising a proprietary x-ray source and x-ray optic optimized for the Single-Wavelength Anomalous Dispersion (SAD) approach to x-ray crystallography. This illumination beam system is designed to deliver x-ray energies that optimize the signal of commonly used scatterers in the SAD technique, and will furthermore provide 8X higher flux than current state-of-the-art laboratory x-ray sources. The key innovations include a novel pattern and new materials for the x-ray source targets and an axially symmetric x-ray optical train that provides a small crossfire for the illumination beam. This proposed phase II project will result in the development of a prototype of the illumination beam system and experimental demonstration of the intended specifications and parameters.

标题：高性能，高吞吐量实验室X射线照明系统，用于蛋白质Crystallragraphy 项目摘要和摘要 X射线蛋白晶体学是确定大分子的3D结构的主要方法（蛋白质，病毒蛋白酶，核酸）。现在，是食品和农业，蛋白质工程和药物设计中工业应用的重要工具。这导致了有关癌症和阿尔茨海默氏症治疗剂的较高较高的后期俱乐部试验。我们建议开发一个X射线照明系统，其中包括专有的X射线源和X射线光学。对单波长第三次（SAD）进行了优化。照明光束系统旨在传递X射线能量，以优化使用的通讯信号悲伤的技术中的散射器，此外，将比当前的最新范围高8倍实验室X射线来源。目标和轴向对称的X射线光学序列，可为照明光束提供小型交火。该提出的II期项目将导致开发照明光束系统的原型以及预期规格和参数的实验证明。