The Biomarker & Analytical Research Core

生物标志物

• 批准号: 9136278
• 负责人: DANIEL T STEIN
• 金额: $ 16.31万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE, INC
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9136278
• 关键词: Affinity; American; Amino Acids; Anabolism; Animals; Area; Automation; Award; Biological Assay; Biological Markers; Biological Preservation; Blood flow; C-Peptide; Carbohydrates; Chemistry; Clinical; Clinical Research; Clinical Sciences; Collaborations; Core Facility; Coupled; DNA; Data; Databases; Diabetes Mellitus; Enzymes; Equipment; Fatty Acids; Financial Support; Fostering; Funding; Glucose; Goals; Gold; Heart; Hepatic; Hormones; Human; Human Resources; Immunoassay; Institutes; Insulin; Kinetics; Label; Laboratories; Management Information Systems; Mass Fragmentography; Measurement; Measures; Medical center; Metabolism; Methodology; Methods; Molecular Biology; Obesity; Output; Pathology; Patients; Physiological; Plasma; Process; Proteins; Proteomics; RNA; Radioactive; Radioimmunoassay; Recording of previous events; Recovery; Research; Research Design; Research Personnel; Research Training; Resources; Robot; Robotics; Rodent; Running; Sampling; Scientist; Secure; Services; Source; Staging; Steroids; System; Techniques; Technology; Translational Research; Triglycerides; United States National Institutes of Health; University Hospitals; Work; base; computerized; cost effective; cost efficient; flexibility; glucose disposal; glucose production; human study; human subject; improved; innovation; insulin secretion; liquid chromatography mass spectrometry; member; metabolomics; novel; polypeptide C; response; stable isotope; steroid hormone; technology development

The Biomarker Analytical Research Core (BARC) provides cost effective and efficient analyses, methodologies to promote the diabetes research efforts of the Einstein-Mount Sinai Diabetes Research Center (ES-DRC) spanning the translational spectrum. The BARC integrates high quality sample extraction and processing, computerized sample tracking using a laboratory information management system (LIMS), high-throughput robotics for hormone assays and metabolite measurements, gas chromatography/mass spectrometry (GC/MS) and liquid chromatography/mass spectrometry (LC/MS) measurement of metabolites, steroids and protein hormones. Specific areas of expertise include high-throughput parallel assay measurements, stable isotope measurements of steroids, C-peptide and C-peptide kinetics, and insulin biosynthesis measurements in human subjects. The BARC also coordinates and enhances measures of stable isotope glucose, fatty acid, triglyceride and amino acid flux in humans and rodents with the flux/metabolomic measurements performed in the Stable Isotope & Metabolomics Core. To accomplish these goals, the BARC will: 1) provide high quality, validated immunoassays using high throughput semi-automated sample handling, parallel assay measurements from single thawed source samples (animal or human), and chain of custody data output; 2 provide specialized measurements (gold standard or reference methods) from human biofluids of physiological carbohydrates, steroid hormones, and C-peptide by GC/MS or LC/MS quantification; 3) provide measurements of radioactive metabolites labeled with 3H and 14C using high-throughput gamma counting suitable for animal and human physiologic turnover studies and radioimmunoassay; 4) provide specialized equipment for high efficiency extraction while preserving analyte integrity of RNA, DNA and proteins for molecular biology, proteomic and biomarker analyses; 5) provide guidance for proper study design for the measurement in human study subjects of hepatic glucose production, whole body glucose disposal, fatty acid, triglyceride and amino acid turnover, hepatic blood flow, insulin secretion and insulin biosynthesis; and 6) coordinate these efforts with other ES- DRC and Institutional Core facilities at Einstein and Mount Sinai. All of these services are available to investigators new to diabetes research, as well as to investigators working on diabetes-related projects that can be enriched and extended by the use of the expertise and facilities of this Core.

生物标志物分析研究核心（BARC）提供了具有成本效益，有效的分析，方法论 为了促进爱因斯坦 - 山西奈糖尿病研究中心（ES-DRC）的糖尿病研究工作 跨越翻译光谱。 BARC整合了高质量的样品提取和处理， 使用实验室信息管理系统（LIMS），高通量的计算机样品跟踪 用于激素测定和代谢物测量的机器人技术，气相色谱/质谱法（GC/MS） 以及代谢物，类固醇和蛋白质的液相色谱/质谱（LC/MS）测量 激素。特定的专业知识领域包括高通量平行测定测量值，稳定的同位素 人类类固醇，C肽和C肽动力学以及人类胰岛素生物合成测量的测量 主题。 BARC还可以协调并增强稳定的同位素葡萄糖，脂肪酸，甘油三酸酯的量度 在人类和啮齿动物中的氨基酸通量，并具有在稳定中进行的通量/代谢组测量 同位素和代谢组学核心。为了实现这些目标，BARC将：1）提供高质量，经过验证 使用高吞吐量的半自动化样品处理，并行测定测量的免疫测定 单一解冻的源样本（动物或人类）和保管数据输出链； 2提供专业 来自生理碳水化合物的人类生物流体的测量（黄金标准或参考方法）， 类固醇激素和GC/MS或LC/MS定量的C肽； 3）提供放射性的测量 使用适合动物和人类的高通量伽玛计数标记为3H和14C的代谢产物 生理周转研究和放射免疫分析； 4）提供高效率的专业设备 提取分子生物学，蛋白质组学和 生物标志物分析； 5）为人类研究对象进行适当的研究设计提供指导 肝葡萄糖的产生，全身葡萄糖处置，脂肪酸，甘油三酸酯和氨基酸更新， 肝血流，胰岛素分泌和胰岛素生物合成； 6）与其他ES-协调这些努力 爱因斯坦和西奈山的刚果民主共和国和机构核心设施。所有这些服务都可以使用 糖尿病研究的新研究人员以及从事与糖尿病有关的项目的研究人员 可以通过使用该核心的专业知识和设施来丰富和扩展。