Structured illumination multispectral fluorescence lifetime microendoscopy to detect oral premalignancies

结构照明多光谱荧光寿命显微内窥镜检测口腔癌前病变

• 批准号: 8976837
• 负责人: Bilal Malik
• 金额: $ 7.43万
• 依托单位: TEXAS ENGINEERING EXPERIMENT STATION
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-12-02 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8976837
• 关键词: Accounting; American; American Cancer Society; Basal Cell; Benign; Biochemical; Biological; Biological Markers; Carcinoma; Cervical; Characteristics; Cheek structure; Collagen; Collection; Colorectal; Detection; Development; Devices; Diagnosis; Diagnostic; Disease; Drops; Early identification; Endoscopes; Endoscopy; Epithelial; Epithelium; Evaluation; Exhibits; Family suidae; Fluorescence; Generations; Goals; Hamsters; Health; Image; Imaging Techniques; In Vitro; Keratin; Lamina Propria; Lesion; Light; Lighting; Malignant - descriptor; Malignant Neoplasms; Metabolic; Modeling; Modification; Molecular; NADH; Optics; Oral; Oral Examination; Oral cavity; Outcome; Patients; Performance; Pharyngeal structure; Physiological; Porphyrins; Premalignant; Process; Reporting; Research; Screening for Oral Cancer; Sensitivity and Specificity; Signal Transduction; Site; Specificity; Speed; Squamous cell carcinoma; Staging; Structural Genes; Structure; Survival Rate; System; Techniques; Testing; Three-Dimensional Image; Tissue imaging; Tissues; United States; Work; adaptive optics; attenuation; base; carcinogenesis; cofactor; design; fluorophore; imaging system; improved; in vivo; instrument; intraepithelial; liquid crystal; malignant mouth neoplasm; molecular marker; optical imaging; oral carcinogenesis; oral lesion; oral premalignancy; screening; tissue phantom; Accounting; American; American Cancer Society; Basal Cell; Benign; Biochemical; Biological; Biological Markers; Carcinoma; Cervical; Characteristics; Cheek structure; Collagen; Collection; Colorectal; Detection; Development; Devices; Diagnosis; Diagnostic; Disease; Drops; Early identification; Endoscopes; Endoscopy; Epithelial; Epithelium; Evaluation; Exhibits; Family suidae; Fluorescence; Generations; Goals; Hamsters; Health; Image; Imaging Techniques; In Vitro; Keratin; Lamina Propria; Lesion; Light; Lighting; Malignant - descriptor; Malignant Neoplasms; Metabolic; Modeling; Modification; Molecular; NADH; Optics; Oral; Oral Examination; Oral cavity; Outcome; Patients; Performance; Pharyngeal structure; Physiological; Porphyrins; Premalignant; Process; Reporting; Research; Screening for Oral Cancer; Sensitivity and Specificity; Signal Transduction; Site; Specificity; Speed; Squamous cell carcinoma; Staging; Structural Genes; Structure; Survival Rate; System; Techniques; Testing; Three-Dimensional Image; Tissue imaging; Tissues; United States; Work; adaptive optics; attenuation; base; carcinogenesis; cofactor; design; fluorophore; imaging system; improved; in vivo; instrument; intraepithelial; liquid crystal; malignant mouth neoplasm; molecular marker; optical imaging; oral carcinogenesis; oral lesion; oral premalignancy; screening; tissue phantom

 DESCRIPTION (provided by applicant): The American Cancer Society estimates that more than 40,000 new cases of invasive cancer in the oral cavity and pharynx are reported each year in the US, and five-year survival rates remain below 40% for advanced stage disease. The goal of this research is to design, build and test a structured illumination-enabled multispectral widefield fluorescence lifetime imaging microendoscope for detection of oral precancer in vivo. Combining structured illumination (SI) with fluorescence lifetime imaging (FLIM) would enable generation of high fidelity three dimensional volumes of the fluorescence intensity and lifetime of the epithelial tissue. We believe that the ability to collect 'optical sections' across the epithelum would allow characterization of tissue biochemical composition due to differential distribution of molecular markers, such as structural proteins and metabolic cofactors, within tissue and will ultimately aid in high sensitivity and specificity detection of early oral cancer development. The specific aims of this proposal are to: 1) construct a structured illumination multispectral FLIM endoscope (SI-FLIM) system to provide optical depth sectioning; 2) characterize the performance and optimize the design specifications of the SI-FLIM endoscope by imaging in vitro and ex vivo; and 3) validate and demonstrate the capability of SI-FLIM by imaging tissue in vivo. The successful completion of this work will enable the development of the first multispectral SI-FLIM microendoscope with the ability to selectively image specific depths within the tissue, and will demonstrate its potential for non-invasive characterization of oral premalignant and malignant lesions. With the wealth of specific molecular information encoded in the collected fluorescence intensity and lifetime in multiple spectral channels, such a device can serve to provide an objective screening of the oral cavity in addition to the clinician's subjective evaluation. Although the proposed endoscope is designed for application of this device in the oral cavity, the SI-FLIM system can be applicable to detect and diagnose other carcinomas (e.g. basal cell, colorectal, and cervical) as well with slight modifications to the endoscope.

 描述（由适用提供）：美国癌症协会估计，在美国，每年在口腔和咽部中有40,000例新的侵入性癌症病例，而晚期疾病的五年生存率仍低于40％。这项研究的目的是设计，构建和测试具有结构化照明的多光谱宽场荧光寿命成像微镜，以检测体内口服预科剂。将结构化照明（SI）与荧光寿命成像（FLIM）相结合，可以产生高保真度的三维体积的荧光强度和寿命 上皮组织。我们认为，由于分子标记物的不同分布（例如结构蛋白和代谢辅助因子）在组织内，在整个上皮收集“光学切片”的能力将允许组织生化组成，并最终有助于高敏感性和早期口腔癌发育的特异性检测。该提案的具体目的是：1）构建一个结构化照明多光谱FLIM内窥镜（SI-FLIM）系统，以提供光学深度分段； 2）表征性能并通过体外成像成像SI-FLIM内窥镜的设计规范； 3）通过在体内成像组织来验证和证明SI-FLIM的能力。这项工作的成功完成将使第一个多光谱SI-FLIM微型内镜的发展能够选择性地图像组织内的特定深度，并将证明其对口服前态和恶性病变的非侵入性表征的潜力。通过在多个光谱通道中收集的荧光强度和寿命中编码的大量特定分子信息，此类设备还可以提供对口腔的客观筛查，除了临床的主观评估。尽管所提出的内窥镜设计用于在口腔中应用该设备，但SI-FLIM系统也可以用于检测和诊断其他癌（例如基本细胞，结直肠和宫颈）以及对内窥镜的稍微修饰。