Biostatistics Module

生物统计模块

• 批准号: 9120884
• 负责人: MAE O GORDON
• 金额: $ 4.08万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9120884
• 关键词: Animals; Applications Grants; Area; Biological Models; Biometry; Biostatistics Core; Clinical Research; Collaborations; Communities; Core Grant; Cryopreservation; DNA; Diagnosis; Dissection; Electron Microscopy; Electronics; Embryo; Equipment; Eye diseases; Fluorescence; Fostering; Funding; Gene Targeting; Goals; Image; In Situ Hybridization; Individual; Knockout Mice; Lasers; Measurement; Microinjections; Microscope; Molecular Genetics; Morphology; Mus; Optical Coherence Tomography; Optometry; Polymerase Chain Reaction; Preparation; Production; Publications; Reporting; Research; Research Design; Research Methodology; Research Personnel; Resource Sharing; Resources; Scientist; Services; Source; System; Technology; Testing; Tissues; Training; Transgenic Mice; Universities; Vision; Vision research; Washington; design; immunocytochemistry; in vivo imaging; instrumentation; light microscopy; repaired; sperm cell; visual performance

Project Summary The objective of this application is to provide established NEI-funded researchers with additional, shared support to enhance their individual research capabilities. A further goal is to enhance the research capability of Washington University by fostering collaborative studies and attracting scientists new to vision research. These objectives will be achieved by operating four research/service cores, which will provide the following services. 1. A Morphology and Imaging Core will provide technical support in the preparation of ocular tissues for analysis by light or electron microscopy. The core also provides in situ hybridization, immunocytochemistry and laser micro-dissection. Core investigators have unlimited access to confocal and multiphoton microscopes. For in vivo imaging/measurements, a fluorotron, fluorescence macroscope, and small animal Optical Coherence Tomography (OCT) system are available. 2. A Visual Function Testing Core will provide expertise, instrumentation, and training on technologies used to quantify visual performance in mice and other model systems. ERG, VEP and optometry analysis are supported. This core also includes technical support for the fabrication, diagnosis and repair of electronic equipment. 3. A Biostatistics Core will provide statistical and methodological expertise in study design and assure the validity of statistical analyses and reported results. This core will also assist in the training of residents and clinicians in areas of clinical research methodology. 4. A Molecular Genetics Core will provide customized services for the production of transgenic and knockout mice using Crispr/Cas9 technology. Supported services also include assistance with design and preparation of constructs for gene targeting, preparation of DNA clones and probes, design of polymerase chain reaction components, microinjection services (IVF), and sperm/embryo cryopreservation. Provision of these support services and resources will greatly enhance the research capabilities of investigators at Washington University and will facilitate collaboration among new and established vision scientists.

项目摘要 该应用的目的是向已建立的Nei资助的研究人员提供 其他共同的支持以增强其个人研究能力。另一个目标是 通过培养合作研究和 吸引刚开始视力研究的科学家。这些目标将通过操作四个来实现 研究/服务核心将提供以下服务。 1。形态学和成像核心将为准备技术提供技术支持 眼组织通过光或电子显微镜进行分析。核心还提供原位 杂交，免疫细胞化学和激光微脱落。核心调查人员拥有 无限访问共聚焦和多光子显微镜。用于体内 成像/测量，荧光子，荧光宏观镜和小动物 可以使用光学相干断层扫描（OCT）系统。 2。视觉功能测试核心将提供专业知识，仪器和培训 用于量化小鼠和其他模型系统的视觉性能的技术。 支持ERG，VEP和验光分析。该核心还包括技术 支持电子设备的制造，诊断和修复。 3。生物统计学核心将提供研究的统计和方法论专业知识 设计并确保统计分析的有效性和报告的结果。这个核心将 还可以协助培训临床研究领域的居民和临床医生 方法论。 4。分子遗传学核心将为生产提供定制的服务 使用CRISPR/CAS9技术的转基因和淘汰小鼠。也支持服务 包括在设计和准备基因靶向的构造方面的帮助， DNA克隆和探针的制备，聚合酶链反应的设计 组件，微注射服务（IVF）和精子/胚胎冷冻保存。 提供这些支持服务和资源将大大增强研究能力 华盛顿大学调查人员的工作，将促进新的和 建立的视力科学家。