Oxidative Lipidomics Core

氧化脂质组学核心

• 批准号: 8643333
• 负责人: Valerian E Kagan
• 金额: $ 20.13万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-01-03 至 2018-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8643333
• 关键词: Acute Lung Injury; Adult Respiratory Distress Syndrome; Bacteria; Biochemical Pathway; Biological Markers; Cardiolipins; Cell membrane; Cells; Development; Goals; Image; Individual; Infection; Knowledge; Lipid Peroxidation; Lipids; Liquid Chromatography; Lung; Mass Spectrum Analysis; Mediator of activation protein; Metabolic Pathway; Mitochondria; Modality; Molecular; Pathogenesis; Performance; Phospholipids; Protocols documentation; Research Personnel; Sampling; Signal Transduction; Structure of parenchyma of lung; Techniques; Therapeutic; Thin Layer Chromatography; Toxin; Training; Work; base; design; innovation; instrumentation; novel; novel therapeutics; oxidation; oxidized lipid; peroxidation; research study; response

Currently, there is no effective pharmacologic therapy against ARDS because of insufficient knowledge of the pathogenesis mechanisms. The overarching focus of the P01 is on mechanisms of pulmonary responses to a new pulmonary toxin and regulator, the anionic phosholipid, cardiolipin (CL) originating from bacteria or mitochondria of damaged host cells during acute lung injury. Thus elucidation of novel metabolic and biochemical pathways of different molecular species of CL, its metabolites and their interactions with other pulmonary lipids and their peroxidation products are fundamental to all four projects of the P01. The Oxidative Lipidomics Core (Core B) has been designed to allow researchers in the projects to perform detailed analysis - identification, characterization and imaging - of all major molecular species of Cls, other different classes of lipids as well as their oxidation products. This will be achieved by using sophisticated state-of-the art techniques based on different versions of mass-spectrometry (MS) combined with liquid¿ chromatography (LC) or high-performance thin-layer chromatography (HPTLC) protocols. Specific Aims of the Oxidative Lipidomics Core B are to: 1. Provide professional expertise in the design and implementation of experiments using adequate techniques for identification, characterization, and quantification of lipids, particularly Cls. Prepare and optimally analyze samples to detect individual molecular species of lipids and oxidized lipids. 2. Provide opportunities for mass-spectrometric imaging of different types of individual molecular species and oxidized lipids in lung tissues. 3. Evaluate experimental results and propose subsequent experimental direction. 4. Provide training in the use of and access to any instrumentations and techniques used within the P01 project to assess lipidomics/oxidative lipidomics biomarkers. By assisting the Projects in the analytical work, the Core will facilitate studies of the mechanisms through which CL functions as a new molecular signal in acute lung injury hence contributes to the development of new therapeutic modalities.

当前，由于对发病机制的了解不足，因此没有针对ARD的有效药物治疗。 P01的总体重点是对新的肺毒素和调节剂的肺反应机制，即急性肺损伤期间细菌或线粒体受损的宿主细胞的阴离子phosholipid，CL）的phosholipid（CL）。阐明了不同分子的Cl，其代谢产物及其与其他肺脂质的相互作用及其过氧化产物的新代谢和生化途径是P01的所有四个项目的基础。氧化脂质组核心（核心B）旨在使项目中的研究人员能够对所有主要的CLS，其他不同类别的脂质及其氧化产物进行详细的分析 - 识别，表征和成像 - 识别，表征和成像。这将通过使用基于不同版本的质谱法（MS）与液相色谱法（LC）或高性能薄层色谱（HPTLC）方案结合使用的不同版本的质谱法（MS）来实现。氧化脂质组学核心B的具体目的是：1。使用适当的技术在设计和实施实施方面提供专业知识，以识别脂质，尤其是CLS的识别，表征和定量。准备和最佳分析样品以检测脂质的单个分子种和氧化脂质。 2。为肺组织中不同类型的单个分子物种和氧化脂质的质谱成像提供了机会。 3。评估实验结果和建议随后的实验方向。 4.提供对P01项目中用于评估脂质组学/氧化脂质组生物标志物中使用的任何仪器和技术的使用和访问的培训。通过协助项目进行分析工作，核心将有助于研究Cl在急性肺损伤中用作新分子信号的机制，因此有助于发展新的治疗方式。