Immunology Core

免疫学核心

基本信息

批准号：
8847269
负责人：
James L Riley
金额：
$ 25.62万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/8847269
关键词：
AIDS clinical trial group AIDS/HIV problem Acquired Immunodeficiency Syndrome Animal Model Basic Science Biological Assay Blood Component Removal Budgets CD4 Positive T Lymphocytes Cell Therapy Cells Clinical Clinical Investigator Clinical Trials Communities Consultations Custom Development Enzyme-Linked Immunosorbent Assay Equipment Flow Cytometry Funding Genes Goals HIV HIV therapy HIV vaccine HIV-1 Human Human Resources IMPAACT Immune Immune response Immune system Immunology Immunotherapeutic agent Individual Infection Instruction Interdisciplinary Study Measurement Mental Health Mentors Mentorship Mission Modeling Mus Natural Killer Cells Pathogenesis Patients Peripheral Blood Mononuclear Cell Play Population Prevention Protocols documentation Reagent Recruitment Activity Research Research Infrastructure Research Personnel Resources Role Services Sorting - Cell Movement T cell therapy T-Lymphocyte Technology Training Training and Education Transfection Translational Research Viral hepatitis Work Zinc Fingers base clinical investigation co-infection cytokine design enzyme linked immunospot assay flexibility in vivo innovation member monocyte mouse model novel novel strategies novel vaccines nuclease outreach reactivation from latency response tool transmission process working group

项目摘要

PROJECT SUMMARY (See instructions): The Immunology Core (Core E), under the direction of Dr. Jim Riley, enables innovative interdisciplinary research on the prevention, pathogenesis and treatment of HIV-1 infection by offering state of the art immunological services, materials, animal models and equipment, and by providing consultation, training and expertise, to basic, translational and clinical investigators in the Penn CFAR community. The Core services include: (1) Purification of primary human bloocl cell subsets from both healthy and HIV-1 infected individuals; (2) Access to and expertise in use of a small animal model of mV-1 infection that can be used to study HIV-1 latent reservoirs, adoptive T cell therapy approaches, transmission bottlenecks and other aspects of HIV pathogenesis and treatment; (3) Full-service immunological assays using ACTG and IMPAACT approved protocols to support clinical trials, clinical investigators or basic science labs without immunological expertise; (4) Flexible, custom-designed immunological assays to support innovative gene- and cell-therapy approaches to treat and/or eradicate HIV-1 infection; (5) Mentoring for emerging investigators, as well as training and education for trainees and lab personnel in a range of basic immunological assays including primary cell transfection, ELISA, ELISPOT, FACS and intracellular cytokine measurement, and other assays, and; (6) Access to cutting-edge equipment including BSL3 sorting and Luminex analysis. To achieve its goals, the Core collaborates closely with other CFAR Cores, and supports a broad range of studies, including innovative

项目摘要（请参阅说明）：在吉姆·赖利（Jim Riley）博士的指导下，免疫学核心（核心E）可以通过提供艺术免疫服务，材料，材料，动物模型和设备，以及通过提供咨询，培训，培训和专业知识，为基本的，翻译和临床研究员提供penn conn COF的创新性跨学科研究HIV-1感染的预防，发病和治疗。核心服务包括：（1）从健康和HIV-1感染的个体中纯化原发性人类蓝骨细胞亚群；（2）使用MV-1感染的小动物模型的获取和专业知识，可用于研究HIV-1潜在储层，产卵T细胞疗法，传播瓶颈以及HIV发病机理的其他方面；（3）使用ACTG和INCACT批准的方案提供全方位服务的免疫学测定，以支持临床试验，临床研究人员或基础科学实验室，而没有免疫专业知识；（4）灵活，定制设计的免疫学测定，以支持创新的基因和细胞治疗方法治疗和/或消除HIV-1感染；（5）在一系列基本的免疫学测定中为新兴研究者提供指导，以及针对学员和实验室人员的培训和教育，包括原发性细胞转染，ELISA，ELISPOT，FACS，FACS和细胞内细胞因子测量，其他测定法，以及其他测定法（6）访问包括BSL3排序和Luminex分析的前沿设备。为了实现其目标，核心与其他CFAR核心紧密合作，并支持广泛的研究，包括创新