Chemical Synthesis and High Throughput Analytics Shared Resource

化学合成和高通量分析共享资源

基本信息

批准号：
8934383
负责人：
GARY ALLEN SULIKOWSKI
金额：
$ 12.2万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-09-17 至 2016-04-29
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8934383
关键词：
Affinity Animals Basic Cancer Research Biochemical Biological Biological Assay Biological Phenomena Cancer Center Cancer Center Support Grant Categories Chemicals Chemistry Code Custom Drug Kinetics Experimental Designs Fluorescent Probes Funding Genes Genome Genus Hippocampus Goals Human Human Genome Human Resources Image In Vitro Individual Knowledge Label Lead Leadership Libraries Malignant Neoplasms Methodology Modeling Nucleotides Pathway interactions Peptides Pharmaceutical Chemistry Pharmaceutical Preparations Phase Play Pre-Clinical Model Preclinical Drug Evaluation Process Research Personnel Research Support Resource Sharing Resources Robotics Role Running Services Small Interfering RNA Structure-Activity Relationship System Techniques Technology Testing Therapeutic Therapeutic Intervention Training Translating Translational Research United States National Institutes of Health Work abstracting anticancer research assay development base chemical synthesis cost effective design detector drug metabolism experience extracellular falls high throughput screening improved in vivo innovation insight instrument instrumentation member new therapeutic target pre-clinical screening small molecule small molecule libraries tool tumor metabolism

Affinity Animals Basic Cancer Research Biochemical Biological Biological Assay Biological Phenomena Cancer Center Cancer Center Support Grant Categories Chemicals Chemistry Code Custom Drug Kinetics Experimental Designs Fluorescent Probes Funding Genes Genome Genus Hippocampus Goals Human Human Genome Human Resources Image In Vitro Individual Knowledge Label Lead Leadership Libraries Malignant Neoplasms Methodology Modeling Nucleotides Pathway interactions Peptides Pharmaceutical Chemistry Pharmaceutical Preparations Phase Play Pre-Clinical Model Preclinical Drug Evaluation Process Research Personnel Research Support Resource Sharing Resources Robotics Role Running Services Small Interfering RNA Structure-Activity Relationship System Techniques Technology Testing Therapeutic Therapeutic Intervention Training Translating Translational Research United States National Institutes of Health Work abstracting anticancer research assay development base chemical synthesis cost effective design detector drug metabolism experience extracellular falls high throughput screening improved in vivo innovation insight instrument instrumentation member new therapeutic target pre-clinical screening small molecule small molecule libraries tool tumor metabolism

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项目摘要

TRANSLATIONAL RESEARCH GROUP CORE 010 – CHEMICAL SYNTHESIS AND HIGH-THROUGHPUT ANALYTICS SHARED RESOURCE PROJECT SUMMARY/ABSTRACT The Vanderbilt Chemical Synthesis and High-throughput Analytics Shared Resource (CSHTASR) provides state-of-the-art capabilities for Vanderbilt-Ingram Cancer Center (VICC) researchers to harness the power of chemistry and whole genome siRNA to support research requiring the application of chemical tools to answer biological questions. Projects supported by this shared resource fall into the categories of: 1) medicinal chemistry, including hit-to-lead and individual chemical probe synthesis for preclinical or hypothesis-driven in vitro studies; 2) large-scale synthesis (10-100 grams) for in vivo animal studies; 3) synthesis of isotopically labeled compounds; 4) affinity and fluorescent probes; 5) synthesis of modified nucleotides and peptides; and 6) high-throughput analytics instrumentation that provide readouts for multiple experimental designs ranging from biochemical to image-based phenotypic assays; 7) whole genome siRNA library screens of up to 75,000 siRNAs; 8) low-, medium- and high-throughput chemical screens of very diverse chemical libraries. The CSHTASR provides these services through a combination of instrument technology and personnel knowledge. Institutional and NIH funds have been invested to acquire leading-edge instrumentation required for effective and efficient CSHTA services. In addition, the CSHTASR’s small molecule and siRNA libraries are valuable material resources that allow interrogation of the biological phenomena being studied, providing insight into target/pathway identification and starting points for chemical probe optimization. The CSHTASR employs a highly trained leadership and support staff with extensive experience in advanced chemistry techniques and HTS methodologies. A consultative approach is used for projects that come through the shared resource; this is a necessary and beneficial model that allows transfer of knowledge between VICC investigator and the shared resource staff to provide the optimal approach to each individual need.

翻译研究小组核心010 - 化学合成和高通量分析共享资源项目摘要/摘要范德比尔特化学合成和高通量分析共享资源（CSHTASR）提供 Vanderbilt-Ingram癌症中心（VICC）研究人员的最先进能力来利用化学和整个基因组siRNA支持需要应用化学工具回答的研究生物学问题。该共享资源支持的项目属于：1）医学化学，包括临床前或假设驱动的命中率和单个化学探针合成体外研究； 2）用于体内动物研究的大规模合成（10-100克）； 3）同位素合成标记的化合物； 4）亲和力和荧光问题； 5）合成修饰的核苷酸和肽；和 6）高通量分析仪器，可为多种实验设计提供读数从生化到基于图像的表型测定； 7）全基因组siRNA图书馆屏幕高达75,000 sirnas; 8）非常多样化的化学文库的低，中和高通量化学筛选。 CSHTASR通过仪器技术和人员知识的结合提供这些服务。已经投资了机构和NIH资金来获取有效的领先工具和有效的CSHTA服务。此外，CSHTASR的小分子和siRNA库有价值允许研究生物学现象的物质资源，从而提供了洞察力目标/途径识别和化学探针优化的起点。 CSHTASR员工A 训练有素的领导力和支持员工在高级化学技术方面拥有丰富的经验， HTS方法。通过共享资源进行的项目使用了一种咨询方法；这是一个必要且有益的模型，可以在VICC研究者和共享资源人员为每个人提供最佳方法。