Assessing Mitochondrial Metabolism by Magnetization Transfer MR Fingerprinting

通过磁化转移 MR 指纹分析评估线粒体代谢

• 批准号: 8975343
• 负责人: Xin Yu
• 金额: $ 19.81万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-07-15 至 2017-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8975343
• 关键词: ATP Synthesis Pathway; Acceleration; Address; Algorithms; Chemical Shift Imaging; Chemicals; Clinical; Computer Simulation; Creatine Kinase; Detection; Development; Diabetes Mellitus; Diagnostic; Dictionary; Diffusion; Disease Progression; Energy Metabolism; Equilibrium; Evolution; Fingerprint; Foundations; Frequencies; Goals; Health; Heart; Image; In Vitro; Knowledge; Laboratories; Lead; Life; Limb structure; Magnetic Resonance; Magnetic Resonance Imaging; Magnetic Resonance Spectroscopy; Maps; Measurement; Measures; Metabolic; Metabolic Diseases; Metabolic syndrome; Metabolism; Methods; Mitochondria; Modeling; Noise; Obesity; Pattern; Phosphocreatine; Phosphorus; Physics; Physiological; Process; Property; Protons; Rattus; Relaxation; Reperfusion Injury; Resolution; Scheme; Signal Transduction; Skeletal Muscle; Spectrum Analysis; Techniques; Therapeutic Effect; Time; Tissues; Translating; Treatment Efficacy; Water; Workload; clinical investigation; data acquisition; design; imaging modality; improved; in vivo; innovation; inorganic phosphate; novel; novel strategies; pre-clinical; research study; response; success; tool; tripolyphosphate; validation studies

DESCRIPTION (provided by applicant): Magnetic Resonance Spectroscopy (MRS) provides a powerful tool to interrogate various aspects to tissue metabolism. In particular, phosphorus-31 (31P) magnetization transfer spectroscopy (MT-MRS) has long been proposed as a means of measuring ATP synthesis rate in vivo. However, current 31P MT-MRS methods require prohibitively long data acquisition time to accurately quantify ATP synthesis rate. On the other hand, recent development of magnetic resonance fingerprinting (MRF) method provides a completely new framework of data acquisition that allows simultaneous measurement of several tissue properties, including relaxation times, at drastically reduced acquisition time. The measurement of chemical exchange rate in MT-MRS involves the measurement of the apparent relaxation time (T1app), i.e., the chemical exchange modified T1 relaxation, which is highly analogous to the measurement of T1 in proton imaging. Therefore, the overall objective of this proposal is to develop and validate novel 31P MT-MRF technique for fast and accurate quantification of ATP synthesis rate in hearts. This project has two specific aims. Aim 1 has three parts: 1) designing and evaluating 31P MT-MRF methods by computer simulation; 2) implementing and optimizing the 31P MT-MRF methods in phantom experiments, and 3) validating the 31P MT-MRF methods against established 31P MT-MRS methods in perfused hearts under varying workload. In Aim 2, the feasibility of performing spatially resolved measurement of chemical exchange rate by 31P MT-MRF will be investigated. A compressed sensing spiral 31P chemical shift imaging (CSI) sequence will be developed that will lead to 92-fold acceleration over the Cartesian CSI method. The 31P MT-MRF CSI method will be validated in rat model of ischemia/reperfusion injury in skeletal muscle. Methods developed in this pre-clinical project will lay the foundation for the development of clinical methods that can be applied to evaluating metabolic function in a variety of metabolic diseases such as diabetes and obesity.

描述（由申请人提供）：磁共振波谱（MRS）提供了一种强大的工具来询问组织代谢的各个方面。特别是，磷-31 (31P) 磁化转移光谱 (MT-MRS) 长期以来一直被提议作为测量体内 ATP 合成率的手段。然而，当前的 31P MT-MRS 方法需要过长的数据采集时间才能准确量化 ATP 合成率。另一方面，磁共振指纹（MRF）方法的最新发展提供了一个全新的数据采集框架，允许在大幅减少采集时间的情况下同时测量多种组织特性，包括弛豫时间。 MT-MRS中化学交换率的测量涉及表观弛豫时间（T1app）的测量，即化学交换修饰的T1弛豫，这与质子成像中T1的测量高度相似。因此，本提案的总体目标是开发和验证新型 31P MT-MRF 技术，以快速准确地量化心脏中的 ATP 合成率。该项目有两个具体目标。目标1分为三个部分：1）通过计算机仿真设计和评估31P MT-MRF方法； 2) 在模型实验中实施和优化 31P MT-MRF 方法，以及 3) 在不同工作负荷下的灌注心脏中对照已建立的 31P MT-MRS 方法验证 31P MT-MRF 方法。在目标 2 中，将研究通过 31P MT-MRF 进行化学交换率空间分辨测量的可行性。将开发压缩传感螺旋 31P 化学位移成像 (CSI) 序列，该序列将比笛卡尔 CSI 方法加速 92 倍。 31P MT-MRF CSI 方法将在骨骼肌缺血/再灌注损伤的大鼠模型中得到验证。该临床前项目开发的方法将为开发可用于评估糖尿病和肥胖等多种代谢疾病的代谢功能的临床方法奠定基础。