OUHSC Macromolecular X-ray Facility

OUHSC高分子X射线设备

• 批准号: 8852144
• 负责人: Blaine H. M. Mooers
• 金额: $ 26.85万
• 依托单位: UNIVERSITY OF OKLAHOMA
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至 2016-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8852144
• 关键词: Caliber; Centers of Research Excellence; Core Facility; Crystallography; Data Collection; Equipment; Grant; Individual; Maintenance; Oklahoma; Photons; Preparation; Proteins; Research Project Grants; Roentgen Rays; Schedule; Structure; Techniques; Temperature; Training; United States National Institutes of Health; Work; X-Ray Crystallography; computerized data processing; experience; instrument; member; screening; structural biology

The OUHSC X-ray Core Facility (initiated in 1998 with a NIH IDeA grant to Dr. Paul Weigel) provides expertise and equipment to the individual COBRE projects on a variety of techniques critical to crystallographic and small angle X-ray scattering studies (SAXS) of proteins and nucleic adds. The techniques include screening crystals for diffraction quality at room temperature, crystal cryo-protectant screening, diffraction data collection, diffraction data processing, space group assignment, structure determination, structure refinement, structure analysis, and figure preparation. The Core Facility will be upgraded to Include a SAXS instrument and the required X-ray generator upgrade which will have a second port available for crystal work. The new generator will have a fourfold greater flux of photons and a smaller diameter beam. Both features are critical for the screening the small, weakly diffracting crystals common in the COBRE research projects. The X-ray Facility Director oversees maintenance of the instruments, scheduling of users, and training users in both SAXS and X-ray crystallography for Jls, other COBRE members, and OSBN members. The Core Facility will be directed by Dr. Blaine Mooers with assistance from co-Directors Dr. Augen Pioszak and Dr. Franklin Hays. They have over 35 years of combined experience in macromolecular crystallography.

OUHSC X射线核心设施（于1998年启动了NIH的想法，保罗·韦格尔（Paul Weigel）提供） 针对各种技术至关重要的各种技术的专业知识和设备 蛋白质和核酸的晶体学和小角度X射线散射研究（SAXS）增加。这 技术包括筛选晶体，以在室温下衍射质量，晶体冷冻保护剂 筛选，衍射数据收集，衍射数据处理，空间组分配，结构 确定，结构改进，结构分析和数字制备。核心设施将是 升级为包括SAXS仪器和所需的X射线发电机升级，该升级将有第二个 端口可用于水晶工作。新发电机将具有四倍的光子通量和一个较小的 直径光束。这两个特征对于筛选小的，弱衍射的晶体很重要 毛病研究项目。 X射线设施主管负责监督工具的维护， 安排用户的安排，以及用于JLS的SAXS和X射线晶体学的培训用户 成员和OSBN成员。核心设施将由Blaine Mooers博士指导 来自Augen Pioszak博士和Franklin Hays博士的联合主任。他们共有35年的合并 大分子晶体学的经验。