Scientific Core 1 (SC1) In Vivo and Imaging Core

科学核心 1 (SC1) 体内和成像核心

• 批准号: 8855091
• 负责人: EUGENE A PODREZ
• 金额: $ 19.56万
• 依托单位: CLEVELAND CLINIC LERNER COM-CWRU
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-15 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8855091
• 关键词: Blood Vessels; Carotid Arteries; Cell Separation; Cells; Clinic; Data; Detection; EMSA; Electron Microscope; Elements; Endothelial Cells; Enzyme-Linked Immunosorbent Assay; Familiarity; Funding; Gel; Harvest; Hemorrhage; Hemostatic function; Image; Imaging technology; Integrin beta3; Lasers; Leadership; Link; Mesenteric Arteries; Microdissection; Microscope; Microscopic; Microscopy; Modeling; Mus; Performance; Procedures; Prostatic Neoplasms; Proteins; Research Institute; Role; Rose Bengal; Scanning; Services; Slide; Smooth Muscle Myocytes; Structure; System; Tail; Thrombosis; Thrombus; Time; abstracting; angiogenesis; base; cell behavior; cell preparation; charge coupled device camera; experience; in vivo; in vivo Model; in vivo imaging; instrument; instrumentation; investigator training; matrigel; programs; research study; scientific organization; success; transmission process

Abstract Scientific Core 1 Scientific Core 1(SC1) provides imaging capabilities that are used broadly throughout the Program Project. The core performs: a) microscopic imaging; b) in vivo thrombosis and angiogenesis models; and c) isolation of cells from mice for ex vivo experiments. Microscopic imaging is by far the major service of this Core. This portion is organized to take advantage of the existing instrumentation and expertise within the Lerner Research Institute of the Cleveland Clinic. The instrumentation includes 6 Leica DM Microscopes, two Leica DMI6000 microscopes for time lapse imaging, a TIRF system, two Leica TCS-SP2 laser scanning confocal microscopes, a Leica SCN 400 slide scanner, a Leica laser microdissection system, LI-COR Odyssey, laser-based scanning system for gels, in-cell Westerns, ELISA/FLISA, EMSA/Gel Shift, Protein Detection, and a new FEI Tecnai G2 Spirit BioTWIN Transmission Electron Microscope equipped with an 11MPix Gatan Orius 832 CCD camera. Beyond the instrumentation is the true expertise of the Core Co-Director, Dr. Judy Drazba, who has more than 20 years of experience in microscopy, hands-on familiarity with all of the instruments and the ability to train investigators from all of the projects in the appropriate use of instrumentation and in the interpretation of data. The in vivo portion of this core performs thrombosis, hemostasis and angiogenesis models in mice. The models to be performed are FeCl3 and rose Bengal induced thrombosis to the mesenteric and carotid arteries, tail bleeding to quantify hemostasis, and matrigel plug and RM-1 prostate tumor as angiogenesis. The portion of the Core is led by Dr. Eugene Podrez, who is experienced in the performance of these models and led a similar and successful core in the previous funding period. The third element of SC1 performs isolation of murine endothelial cells and smooth muscle cells. These cells are used in two of Projects and having these isolations performed by experienced technologists adds to the success of establishing such cultures and the uniformity of behavior of the cells. By providing skilled leadership and organization, SC1 epitomizes the value that can be gained from centralization of key functions and expertise within a Core within the Program Project.

抽象的 科学核心1 科学核心1（SC1）提供了整个程序项目中广泛使用的成像功能。 核心执行：a）微观成像； b）体内血栓形成和血管生成模型； c）隔离 来自小鼠的细胞进行体内实验。微观成像是迄今为止该核心的主要服务。这 组织的部分是为了利用Lerner研究中现有的仪器和专业知识 克利夫兰诊所研究所。该仪器包括6个Leica DM显微镜，两个Leica DMI6000 延时成像的显微镜，一个TIRF系统，两个Leica TCS-SP2激光扫描共聚焦显微镜， LEICA SCN 400幻灯片扫描仪，Leica Laser显微解剖系统，Li-Cor Odyssey，基于激光的扫描 凝胶，西方人，ELISA/FLISA，EMSA/凝胶移位，蛋白质检测系统和新的Fei Tecnai G2系统 精神Biotwin传输电子显微镜，配备了11MPIX GATAN ORIUS 832 CCD摄像头。 超越仪器是核心联合导演的真正专业知识，朱迪·德拉兹巴（Judy Drazba）博士多于 20年的显微镜经验，对所有乐器的动手熟悉以及训练能力 来自所有项目的研究人员适当使用仪器和数据解释。 该核心的体内部分在小鼠中执行血栓形成，止血和血管生成模型。这 要执行的模型是FECL3和玫瑰孟加拉诱导的肠系膜和颈动脉的血栓形成， 尾部出血以量化止血，Matrigel塞和RM-1前列腺肿瘤作为血管生成。部分 核心由尤金·波德雷斯（Eugene Podrez）博士领导，他在这些模型的性能方面经验丰富，并领导 在上一个资金期间相似和成功的核心。 SC1的第三个元素进行隔离 鼠内皮细胞和平滑肌细胞。这些细胞用于两个项目，并拥有这些单元 经验丰富的技术人员进行的隔离增加了建立此类文化的成功和 细胞行为的统一性。通过提供熟练的领导力和组织，SC1体现了价值 可以从计划项目中的核心中的关键功能和专业知识的集中化中获得。