Measurement Error in Population Health Inequity Research using Novel Biomeasures-Supplement

使用新型生物措施进行人口健康不平等研究中的测量误差-补充

基本信息

批准号：
8902356
负责人：
Arline T Geronimus
金额：
$ 6.25万
依托单位：
UNIVERSITY OF MICHIGAN AT ANN ARBOR
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-04-15 至 2017-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8902356
关键词：
Address Adolescent Adult Age Aging Area Atherosclerosis Behavioral Behavioral Genetics Biological Biological Process Biology Blood Blood Cells Blood specimen Cell Size Cells Child Clinical Complex DNA Data Data Collection Data Set Development Environment Epidemiology Ethnic Origin Family Future Goals Health Health Surveys Heterogeneity Histocompatibility Testing Human Herpesvirus 4 Learning Length Leukocytes Life Cycle Stages Life Experience Link Longitudinal Studies Measurement Measures Mediating Mexican Michigan Molecular Molecular Biology Neighborhoods Not Hispanic or Latino Participant Physical environment Physiological Population Population Research Population Study Poverty Areas Process Public Health Race Recruitment Activity Research Research Personnel Retirement Saliva Sample Size Sampling Sampling Studies Scientist Social Environment Socioeconomic Status Specimen Stress Structure Telomerase Testing United States Universities Validity and Reliability Variant Venous Venous blood sampling Woman Work base behavioral health cost health disparity improved interest novel population based population health psychosocial public health relevance racial and ethnic repository social social disparities socioeconomics telomere theories time use

项目摘要

DESCRIPTION (provided by applicant): Progress in eliminating health disparities requires interdisciplinary efforts among social and biological scientists, and population-based data sets through which complex hypotheses linking environmental, psychosocial, behavioral and biological processes can be tested. The proposed project is an interdisciplinary collaborative effort integrating expertise in population health disparities, molecular biology, and the statisticl impact of measurement error on parameter estimates directly pertinent to population health research. We will address the question of whether DNA from blood or saliva banked with large population based data sets is valid for use in population health studies of telomere length (TL) - an intriguing new biomeasure of stress- mediated health, development, and aging -- and, if so, what sample sizes are needed. Research on population differences in TL is largely based on highly select, racially homogeneous, clinical, or convenience samples. Socioeconomic measures are often absent or rudimentary. The state-of-the-art approach to measuring TL is via leukocyte-derived DNA extracted from fresh venous blood samples. Launching new population-based data collection activities that include blood draws, molecular measurements and the broad swath of social, environmental, behavioral, and health variables needed is costly and takes years before data can be analyzed. However, extant population-based data collections are increasingly isolating blood DNA for storage in specimen repositories after the cells have been transformed and immortalized using Epstein-Barr Virus (EBV) or storing DNA in saliva. Neither approach is ideal for TL measurement. The critical unanswered question for population research is whether the error introduced in these TL measures is random or systematic with respect to original TL or to populations of interest. We propose to estimate the validity of using EBV-immortalized blood cells or saliva cells to estimate population differences in TL. We will collect blood and saliva from 150 adult black, white, or Mexican-origin women in Detroit and Ann Arbor and measure each woman's TL multiple times using DNA directly isolated from fresh blood cells, DNA isolated from cells that we will EBV- immortalize, and DNA extracted from saliva. By stratifying the sample along key axes of comparison - race/ethnicity, socioeconomic status, stress- level, and neighborhood - and making within-woman comparisons on TL between immortalized and fresh blood samples, and between fresh blood samples and saliva, we will directly gauge the effect that immortalization or tissue type has on the validity and reliability o findings from TL studies that use stored specimens and on the future potential use of stored specimens for testing interdisciplinary hypotheses on population health disparities. Findings can be applied toward increasing the pace of health disparities research, whatever the conclusion on the validity of using immortalized blood or saliva cells to measure TL.

描述（由申请人提供）：消除健康差异的进展需要社会和生物科学家之间的跨学科努力，以及基于人群的数据集，通过这些数据集可以测试连接环境、心理社会、行为和生物过程的复杂假设。拟议的项目是一项跨学科合作项目，整合了人口健康差异、分子生物学以及测量误差对与人口健康研究直接相关的参数估计的统计影响方面的专业知识。我们将解决这样一个问题：来自血液或唾液的 DNA 存有大量基于人口的数据集，是否可有效用于端粒长度 (TL) 的人口健康研究——端粒长度是压力介导的健康、发育和衰老的一种有趣的新生物测量方法——如果需要，需要多少样本量。对 TL 人群差异的研究主要基于高度选择性的、种族同质的、临床的或方便的样本。社会经济措施往往不存在或处于初级阶段。最先进的 TL 测量方法是通过从新鲜静脉血样本中提取的白细胞来源的 DNA 进行测量。启动新的基于人群的数据收集活动，包括抽血、分子测量以及所需的广泛社会、环境、行为和健康变量，成本高昂，并且需要数年时间才能分析数据。然而，现有的基于人群的数据收集越来越多地分离血液 DNA，以便在使用 Epstein-Barr 病毒 (EBV) 转化和永生化细胞或将 DNA 存储在唾液中后，将其存储在样本库中。这两种方法都不是 TL 测量的理想选择。人口研究中尚未解决的关键问题是，这些 TL 测量中引入的误差相对于原始 TL 或感兴趣的人口而言是随机的还是系统的。我们建议估计使用的有效性 EBV 永生化血细胞或唾液细胞可估计 TL 的群体差异。我们将从底特律和安娜堡的 150 名成年黑人、白人或墨西哥裔女性身上采集血液和唾液，并使用直接从新鲜血细胞中分离的 DNA（从我们将 EBV 永生化的细胞中分离出的 DNA）多次测量每个女性的 TL。以及从唾液中提取的DNA。通过沿着关键的比较轴（种族/民族、社会经济地位、压力水平和邻里）对样本进行分层，并对永生血样本和新鲜血液样本之间以及新鲜血液样本和唾液之间的 TL 进行女性内部比较，我们将直接评估永生化或组织类型对使用储存标本的 TL 研究结果的有效性和可靠性的影响，以及对未来使用储存标本测试人口健康差异跨学科假设的潜在用途的影响。无论使用永生化血液或唾液细胞测量 TL 的有效性的结论如何，研究结果都可用于加快健康差异研究的步伐。