EXPOSOME BIOCHEMICAL ANALYSIS FOR BIRTH WEIGHT/OBESITY

出生体重/肥胖的暴露生化分析

• 批准号: 8933137
• 负责人: MICHAEL TSAI
• 金额: $ 30.32万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-29 至 2015-09-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8933137
• 关键词: Albumins; Aliquot; Alkaline Phosphatase; Amino Acids; Antibodies; Apolipoproteins B; Bile Acids; Bilirubin; Biochemical; Birth Weight; C-Peptide; CCL2 gene; Cardiovascular Diseases; Carotenoids; Chemicals; Cholecalciferol; Cholesterol; Chronic Disease; Cohort Studies; Collection; Conceptions; Creatinine; Databases; Development; Discipline of obstetrics; E-Selectin; Elderly; Endoglin; Ensure; Enzyme-Linked Immunosorbent Assay; Estradiol; Exposure to; F2-Isoprostanes; FABP4 gene; Fatty Acids; Ferritin; First Pregnancy Trimester; Gestational Age; Glucose; Goals; High Density Lipoproteins; Hippuric acid; Homocysteine; Homocystine; Human; Human Chorionic Gonadotropin; Human Development; Infant; Insulin; Insulin-Like Growth Factor Binding Protein 3; Intercellular adhesion molecule 1; Interleukin-10; Interleukin-6; Investigation; Leptin; Life; Light; Link; Lipoprotein (a); Low-Density Lipoproteins; Measures; Methodology; Morbidity - disease rate; National Institute of Child Health and Human Development; Non-Insulin-Dependent Diabetes Mellitus; Nonesterified Fatty Acids; Nuclear; Obesity; Outcome; Phospholipids; Physiological; Plasminogen Inactivators; Population; Positioning Attribute; Pregnancy; Pregnant Women; Prevalence; Prevention strategy; Progesterone; Proteins; Published Comment; Reproduction; Research; Research Design; Research Personnel; Resources; Risk; SHBG gene; Sampling; Serum; Small for Gestational Age Infant; Somatomedins; TSH receptor antibody; Testosterone; Thyroglobulin; Thyroglobulin antibody; Tocopherols; Transferrin Receptor; Triglycerides; Tumor Necrosis Factor-alpha; Urea Nitrogen; Uric Acid; Urine; Variant; Vascular Cell Adhesion Molecule-1; Vascular Endothelial Growth Factors; Very low density lipoprotein; Vitamin D2; Woman; Women' s Group; Work; acylcarnitine; adiponectin; analytical method; cohort; hepcidin; high risk; human IGFBP2 protein; human TNF protein; implantation; improved; inhibin B; leptin receptor; metabolomics; mortality; mullerian-inhibiting hormone; nephrin; obesity risk; offspring; oxidized low density lipoprotein; podocalyxin; population health; post gamma-globulins; prospective

The Division of Intramural Population Health Research, NICHD, is uniquely positioned to be on the forefront of exposome research, in that its intramural designation is consistent with the conduct of high risk research, many prospective pregnancy cohorts that have generated rich databases with longitudinally collected (in varying degrees) biospecimens during sensitive windows of human development are available, and the Division has biostatisticians with the level of expertise needed to develop appropriate analytical methods. These resources afford Division investigators an opportunity to design research initiatives within the exposome paradigm. Perhaps one of the earliest and most critical sensitive windows occurs during human reproduction and development. Currently the Division has a proof-of-concept study underway to characterize and quantify the pregnancy exposome using an existing pregnancy cohort study with normal outcomes. The goal of the present study is to build upon our previous work that focuses on the characterization of the normal pregnancy exposome by extending the same methodology to 'normal' pregnant women whose infants were born at the extremes of birth weight, as women with offspring small for gestational age (SGA) defined as birth weight < 10th percentile and large for gestational age (LGA) defined as birth weight > 90th percentile (Brenner, 1976) were excluded from the prior analysis. [20] Both low and high birth weight are associated with increased short term morbidity and mortality,and also have been repeatedly related to Increased risk of chronic diseases in later life, such as cardiovascular disease and type 2 diabetes. [21] Comprehensive Investigation of the exposome related to birth weight would potentially improve our understanding or early life determinants of the chronic diseases and help develop early preventive strategies of life long chronic diseases. Evidence has also linked the long-term impact of exposure to maternal obesity to increase risk of obesity and cardiometabolic risk. [22] Of added note is our effort to ensure comparable sampling frameworks for both groups in that all eligible women have their Initial biospecimens collected <13 weeks gestation and during each trimester. Restricting to women with a first trimester biospecimen collection is critical for assessing longitudinal changes during pregnancy, as pregnancy induces many physiologic changes following conception and implantation. Thus, having two randomly selected groups of 'normal’ pregnant women who differ only in terms of infants' birth weight will allow us to characterize and quantify the exposome during pregnancy for each of these two samples of pregnant women. This strategy was a part of our initial work presented to the Division for FY13, but later restricted to only the former group in light of concerns about the integrity of the biospecimens for research purposes. We now demonstrate that the sample integrity is there, and variation can be quantified. Our second objective is to sample a third group of 'normal' pregnant women who are obese and who are most relevant given the increasing prevalence of obesity in contemporary obstetric populations.

壁内人群健康研究NICHD的划分是独特地位于暴露研究的最前沿，因为其壁内设计与高风险研究的进行是一致的，许多潜在的怀孕同龄人同时生成了纵向的数据库，这些数据库已与纵向收集到的纵向级别（在不同程度上的生物范围）所需要的人类开发的生物范围是人类开发的范围，并且是人类开发的生物范围，而人类开发的生物范围是人类的分裂，而不是人类的生物范围。分析方法。这些资源为部门调查人员提供了一个在Exposome范式内设计研究计划的机会。 也许是在人类繁殖和发展过程中最早，最关键的窗户之一。目前，该部门正在进行一项概念验证研究，以使用现有的妊娠队列研究表征和量化妊娠暴露体的表征和量化。本研究的目的是基于我们先前的工作，该工作重点是通过将相同的方法扩展到“正常”的孕妇的正常怀孕表征，这些妇女的婴儿出生于出生体重极端，因为后代小的妊娠年龄（SGA）被定义为出生体重<10个百分位的胎龄（sGA），并在妇女<10个百分点（lga）定义为90> 90的妇女，> 90> brenter> 90， 分析。 [20]低出生率和高出生体重都与短期发病率和死亡率增加有关，并且与以后的慢性疾病风险增加有关，例如心血管疾病和2型糖尿病。 [21]对与出生体重有关的暴露体的全面研究可能会改善我们对慢性疾病的理解或早期生活的决定，并有助于制定长期慢性疾病的早期预防策略。证据还与孕产妇肥胖的长期影响有关，以增加肥胖和心脏代谢风险的风险。 [22]附加说明是我们的努力，以确保两组的可比较抽样框架，因为所有合格的妇女的最初生物晶体均收集了<13周的妊娠和每个孕期。限制孕妇收集生物植物的女性对于评估怀孕期间的纵向变化至关重要，因为怀孕在概念和植入后会引起许多生理变化。这是有两个随机选择的“正常”孕妇的群体，这些孕妇仅在婴儿的出生体重方面有所不同，这将使我们能够对这两个孕妇的两个样本中的每一个中的怀孕期间的暴露和量化。这项策略是我们最初向该部门提交的最初工作的一部分，但后来仅限于对研究目的的生物测量的完整性的担忧。现在，我们证明了样本完整性存在，并且可以量化变化。我们的第二个目标是对肥胖的第三组“正常”孕妇进行采样，鉴于当代产科种群的肥胖症的越来越多，这是最重要的。