Investigation of Horizontal Transfer of Carbapenemase Gene in Gram Negative Bacteria

革兰氏阴性菌碳青霉烯酶基因水平转移的研究

• 批准号: 8952890
• 负责人: Karen Frank
• 金额: --
• 依托单位: CLINICAL CENTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8952890
• 关键词: Bacteria; Bacterial Genome; Data; Disease Outbreaks; Epidemiology; Genes; Goals; Gram-Negative Bacteria; In Vitro; Investigation; Klebsiella; Klebsiella pneumonia bacterium; Morbidity - disease rate; Multi-Drug Resistance; Mus; Nosocomial Infections; Organism; Patients; Pharmacologic Substance; Phenotype; Plasmids; Play; Resistance; Role; Sequence Analysis; System; Time; antimicrobial drug; base; carbapenem resistance; carbapenemase; high throughput screening; improved; in vivo; mortality; pathogen; prevent; research study

Klebsiella pneumoniae that produce carbapenemases such as the K. pneumoniae carbapenemase (blaKPC) are an increasingly common multidrug resistant pathogen causing nosocomial infections and outbreaks, with high rates of morbidity and mortality worldwide. Horizontal transfer of carbapenemase-producing plasmids is believed to play a significant role in the spread of multidrug resistant K. pneumoniae and other Gram-negative bacteria. Our experiments are aimed at defining the parameters regulating the spread of carbapenem resistance. Patients can be colonized by KPC+ K. pneumoniae for extended periods of time; however, only some of these patients have evidence of blaKPC containing plasmids transferring to bacteria other than Klebsiella species based on our extensive surveillance system. Understanding any differences between in vitro potential to spread and in vivo evidence of spread will aid our attempts to prevent dissemination of the resistance phenotype.

产生碳青霉酶（例如肺炎甲虫碳纤维烯酶（BLAKPC））的克雷伯菌肺炎是越来越常见的多种耐药性病原体，导致了医生感染和暴发，全球发病率高和死亡率较高。据信，产生碳青霉酶的质粒的水平转移在耐多药的K.肺炎和其他革兰氏阴性细菌的扩散中起着重要作用。我们的实验旨在定义调节碳青霉含量扩散的参数。可以通过KPC+ K.肺炎定植的患者长时间；但是，根据我们广泛的监测系统，只有其中一些患者有blakpc含有质粒转移到klebsiella物种以外的细菌的证据。了解体外潜力传播和体内传播证据之间的任何差异将有助于我们试图防止抗药性表型传播。