Landscape genetics guide NTD interventions: Chagas disease in Ecuador and Peru

景观遗传学指南 NTD 干预措施：厄瓜多尔和秘鲁的恰加斯病

• 批准号: 8625590
• 负责人: MARIO J GRIJALVA
• 金额: $ 40.76万
• 依托单位: OHIO UNIVERSITY ATHENS
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-01 至 2018-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8625590
• 关键词: Adopted; Affect; Area; Biological Factors; Cells; Chagas Disease; Climate; Communities; Complement; Computer Simulation; Databases; Disease; Domiciles; Ecuador; Environment; Environmental Risk Factor; Epidemiology; Evaluation; Extinction (Psychology); Feasibility Studies; Genes; Genetic; Genetic Structures; Genome; Genomics; Genotype; Heterogeneity; In Vitro; Individual; Insect Vectors; Insecta; Intervention; Invaded; Island; Link; Measures; Methodology; Microsatellite Repeats; Mitochondrial DNA; Modeling; Molecular; Parasites; Pattern; Peru; Pilot Projects; Population; Population Density; Population Distributions; Population Genetics; Precipitation; Province; Relative (related person); Resolution; Rhodnius; Risk Factors; Route; Sampling; Scanning; Scheme; Severities; Short Tandem Repeat; Source; South America; Spatial Distribution; Specimen; Structure; Study models; Temperature; Tropical Disease; Trypanosoma cruzi; Tsetse Flies; Variant; anthropogenesis; base; computerized data processing; disease transmission; disorder control; experience; feeding; genetic analysis; genome sequencing; insight; interest; land use; molecular marker; neglect; pathogen; population genetic structure; public health relevance; rural area; sample collection; statistics; theories; tool; transmission process; urban area; vector; vector control

DESCRIPTION (provided by applicant): Neglected Tropical Diseases affect approximately 2.7 billion people worldwide and are widely distributed, especially among the tropics. The dispersal and distribution of the pathogens that cause NTDs are strongly influenced by the spatial complexity of their physical and biotic environment. Thus, spatial analyses of abiotic and biotic variables can serve to accurately predict the emergence, occurrence, and spread of disease. Furthermore, vital to effective disease control is an understanding of the extent to which populations of parasites and their vectors are connected. In this project we propose to focus on Chagas disease in an area of disease transmission in Southern Ecuador and Northern Peru. Chagas disease is caused by the single-celled kinetoplastid Trypanosoma cruzi, and in our study region the local insect vector species is Rhodnius ecuadoriensis. Our aim is to establish the population genetic/genomic diversity of both vector and parasite across the study region by high resolution (genome scale) genotyping. To evaluate the interconnectivity of different vector and parasite populations, and to predict potential dispersal routes or barriers to gene-flow, we propose to adopt a landscape genetics approach. 'Landscape genetics' encompasses a body of theory and statistical tools to provide deeper insights into the interactions between landscape features (e.g. elevation, land use, population density) and spatial genetic patterns within and between populations and/or individuals. Our aim is to define what environmental factors and what spatial scales are relevant in defining parasite and vector population distribution. Such descriptions of diversity are key to measuring and predicting dispersal. Our evaluation of barriers to gene flow will feed directly into a rationale for effective local disease control. Furthermore our project will provide a model with which to approach the study of the dispersal and distribution of other NTD agents.

描述（由申请人提供）：被忽视的热带病影响着全世界大约 27 亿人，并且分布广泛，尤其是在热带地区。引起 NTD 的病原体的传播和分布受到其物理和生物环境的空间复杂性的强烈影响。因此，非生物和生物变量的空间分析可以准确预测疾病的出现、发生和传播。此外，对于有效控制疾病至关重要的是了解寄生虫种群及其媒介之间的联系程度。在这个项目中，我们建议重点关注厄瓜多尔南部和秘鲁北部疾病传播地区的恰加斯病。恰加斯病是由单细胞动质体克氏锥虫引起的，在我们的研究区域，当地的昆虫媒介物种是厄瓜多尔红锥虫。我们的目标是通过高分辨率（基因组规模）基因分型确定整个研究区域载体和寄生虫的群体遗传/基因组多样性。为了评估不同媒介和寄生虫种群的相互关联性，并预测潜在的扩散路线或基因流障碍，我们建议采用景观遗传学方法。 “景观遗传学”包含一系列理论和统计工具，可以更深入地了解景观特征（例如海拔、土地利用、人口密度）与种群和/或个体内部和之间的空间遗传模式之间的相互作用。我们的目标是确定哪些环境因素和哪些空间尺度与定义寄生虫和病媒种群分布相关。这种对多样性的描述是衡量和预测扩散的关键。我们对基因流动障碍的评估将直接为有效的当地疾病控制提供依据。此外，我们的项目将提供一个模型，用于研究其他 NTD 媒介的传播和分布。

项目成果

Population genomics and geographic dispersal in Chagas disease vectors: Landscape drivers and evidence of possible adaptation to the domestic setting.

• DOI: 10.1371/journal.pgen.1010019
• 发表时间: 2022-03
• 期刊: PLoS genetics
• 影响因子: 4.5
• 作者: Hernandez-Castro LE;Villacís AG;Jacobs A;Cheaib B;Day CC;Ocaña-Mayorga S;Yumiseva CA;Bacigalupo A;Andersson B;Matthews L;Landguth EL;Costales JA;Llewellyn MS;Grijalva MJ
• 通讯作者: Grijalva MJ

Communication in Neglected Tropical Diseases' elimination: A scoping review and call for action.

• DOI: 10.1371/journal.pntd.0009774
• 发表时间: 2022-10
• 期刊: PLoS neglected tropical diseases
• 影响因子: 3.8

2b-RAD genotyping for population genomic studies of Chagas disease vectors: Rhodnius ecuadoriensis in Ecuador.

用于恰加斯病载体群体基因组研究的 2b-RAD 基因分型：厄瓜多尔的 Rhodnius ecuadoriensis。

• DOI: 10.1371/journal.pntd.0005710
• 发表时间: 2017-07
• 期刊: PLoS neglected tropical diseases
• 影响因子: 3.8
• 作者: Hernandez-Castro LE;Paterno M;Villacís AG;Andersson B;Costales JA;De Noia M;Ocaña-Mayorga S;Yumiseva CA;Grijalva MJ;Llewellyn MS
• 通讯作者: Llewellyn MS

Prediction and Prevention of Parasitic Diseases Using a Landscape Genomics Framework.

使用景观基因组框架预测和预防寄生虫病。

• DOI: 10.1016/j.pt.2016.10.008
• 发表时间: 2017
• 期刊: Trends in parasitology
• 影响因子: 9.6
• 作者: Schwabl,Philipp;Llewellyn,MartinS;Landguth,ErinL;Andersson,Björn;Kitron,Uriel;Costales,JaimeA;Ocaña,Sofía;Grijalva,MarioJ
• 通讯作者: Grijalva,MarioJ

Small subunit ribosomal metabarcoding reveals extraordinary trypanosomatid diversity in Brazilian bats.

• DOI: 10.1371/journal.pntd.0005790
• 发表时间: 2017-07
• 期刊: PLoS neglected tropical diseases
• 影响因子: 3.8
• 作者: Dario MA;Moratelli R;Schwabl P;Jansen AM;Llewellyn MS
• 通讯作者: Llewellyn MS