ELECTRON SPIN RELAXATION OF NITROXIDES

氮氧化物的电子自旋弛豫

• 批准号: 8364093
• 负责人: SANDRA S. EATON
• 金额: $ 0.37万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-09-01 至 2012-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8364093
• 关键词: Data; Dependence; Electrons; Frequencies; Funding; Grant; Label; Measurement; National Center for Research Resources; Oxygen saturation measurement; Peptides; Physiologic pulse; Principal Investigator; Process; Relaxation; Research; Research Infrastructure; Resources; Sampling; Source; Spectrum Analysis; Spin Labels; Technology; Temperature; United States National Institutes of Health; cost; cryogenics; in vivo; nitroxyl; research study; theories

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Understanding of electron spin relaxation is key to predicting feasibility, and optimizing experiments for oximetry, in vivo EPR, and distance measurements by relaxation enhancement and pulsed dipolar spectroscopy (DQC and DEER). There is no theory of the frequency dependence of electron spin relaxation that fits available experimental data. There is also a scarcity of experimental data other than at X-band with which to compare theory. Because of the wide-spread use of nitroxyl radicals, and the availability of various isotopically-substituted nitroxyls, they are selected for this study of relaxation processes. This project investigates the frequency dependence of relaxation in two motional regimes (a) rapid tumbling as is typical of in vivo spectroscopy and (b) immobilized samples at cryogenic temperatures as are used for pulsed dipolar spectroscopy. The Eaton lab has pulse capability from 250 MHz to 34 GHz. ACERT offers 9, 17, 35 and 95 GHz with high power pulses and CW at 170 and 240 GHz. The Eatons have a set of nitroxyl compounds with [^15]N, [^14]N, [^2]H, and [^1]H selective labeling. Spin-labeled peptides and samples for pulsed dipolar spectroscopy are available from other projects at ACERT and DU.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 了解电子自旋弛豫对于预测可行性以及通过弛豫增强和脉冲偶极光谱（DQC 和 DEER）优化血氧饱和度、体内 EPR 和距离测量实验至关重要。没有适合现有实验数据的电子自旋弛豫频率依赖性理论。 除了 X 波段之外，还缺乏可与理论进行比较的实验数据。 由于硝酰基自由基的广泛使用，以及各种同位素取代的硝酰基的可用性，它们被选择用于弛豫过程的研究。 该项目研究了两种运动状态下弛豫的频率依赖性（a）典型的体内光谱快速翻滚和（b）脉冲偶极光谱所用的低温固定样品。 伊顿实验室具有 250 MHz 至 34 GHz 的脉冲能力。 ACERT 提供 9、17、35 和 95 GHz 以及 170 和 240 GHz 的高功率脉冲和 CW。 Eatons 拥有一组带有 [^15]N、[^14]N、[^2]H 和 [^1]H 选择性标记的硝酰基化合物。 用于脉冲偶极光谱的自旋标记肽和样品可从 ACERT 和 DU 的其他项目获得。