NATIVE ESI FOR DETERMINING STOICHIOMETRY OF MULTISUBUNIT COMPLEXES

用于确定多亚基复合物化学计量的原生 ESI

• 批准号: 8361430
• 负责人: Robert Eugene Blankenship
• 金额: $ 0.7万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361430
• 关键词: Aerobic; Anaerobic Bacteria; Biological Models; Biological Process; Cells; Chlorobi; Complex; Energy Transfer; Food; Fourier transform ion cyclotron resonance; Funding; Grant; Harvest; Human; LHX2 gene; Light; Mass Spectrum Analysis; National Center for Research Resources; Photosynthesis; Principal Investigator; Process; Proteins; Research; Research Infrastructure; Resources; Source; Theoretical Studies; United States National Institutes of Health; Work; base; biomedical resource; cost; mass spectrometer; photosystem; stoichiometry; thermophilic bacteria

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Photosynthesis is a central biological process that produces all the food and much of the energy used by human beings. An enormous amount of work has been done to understand the structural basis of light harvesting and the efficiency of the energy-transfer process. Several bacterial light-harvesting (LH) complexes, such as LH1, LH2 and Fenna-Matthews-Olson protein (FMO) have served as model systems for structural, spectroscopic and theoretical studies.. The recently discovered, thermophilic bacterium, Candidatus Chloracidobacterium (C.) thermophilum, belongs to the phylum Acidobacteria, and is the only chlorophototroph found in the phylum so far. Surprisingly, the photosystem of the aerobic C. thermophilum closely resembles that of the green sulfur bacteria (GSB), which are strict anaerobes. However, The mechanism that enables the C. thermophilum cells to carry out phototrophy under aerobic conditions is not yet known. FMO proteins of green sulfur bacteria (Chlorobiales) have been extensively studied using a wide range of spectroscopic and theoretical approaches. The new FMO protein is investigated by several approaches including native electrospray in a Bruker 12 tesla FTICR mass spectrometer.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 光合作用是一个重要的生物过程，产生人类使用的所有食物和大部分能量。为了了解光收集的结构基础和能量转移过程的效率，人们已经做了大量的工作。几种细菌光捕获 (LH) 复合物，例如 LH1、LH2 和 Fenna-Matthews-Olson 蛋白 (FMO)，已成为结构、光谱和理论研究的模型系统。最近发现的嗜热细菌，Candidatus Chloracidobacter (C) .) 嗜热菌，属于酸杆菌门，是迄今为止在该门中发现的唯一的绿光营养菌。令人惊讶的是，需氧嗜热梭菌的光系统与绿硫细菌（GSB）非常相似，绿硫细菌是严格的厌氧菌。然而，嗜热梭菌细胞在有氧条件下进行光养的机制尚不清楚。人们使用各种光谱和理论方法对绿色硫细菌（Chlorobiales）的 FMO 蛋白进行了广泛的研究。新的 FMO 蛋白通过多种方法进行研究，包括在 Bruker 12 特斯拉 FTICR 质谱仪中进行自然电喷雾。