TOP-DOWN MASS SPECTROMETRY FOR THE IDENTIFICATION OF PTM ENSEMBLES

用于鉴定 PTM 整体的自上而下质谱法

• 批准号: 8361411
• 负责人: MICHAEL L GROSS
• 金额: $ 0.94万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-01-01 至 2011-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8361411
• 关键词: Cell Cycle; Cells; Funding; Glean; Grant; Maps; Mass Spectrum Analysis; National Center for Research Resources; Paper; Phosphorylation; Phosphorylation Site; Phosphotransferases; Post-Translational Protein Processing; Principal Investigator; Proteins; Proteomics; Publishing; Research; Research Infrastructure; Resources; Site; Source; Time; United States National Institutes of Health; biomedical resource; cost

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Many papers are published that employ bottom-up proteomics to identify sites of potentail phosphorylation or other posttranslational modification. It is important to know the total ensemble of PTM for a protein at a particular time in the cell cycle. This information cannot be gleaned from the bottom-up strategy. Top down proteomics will be used to interrogate large parts of a protein (>10kDa) to discover the distributions of PTM during on proteins isolated from cells. The workflow will be developed by studying Chk2, an autophosphorylating kinase with more than 30 sites of phosphorylation. We will map out the distribution of phosphorylation from Chk2 captured at various times during the cell cycle.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 许多发表的论文采用自下而上的蛋白质组学来识别潜在磷酸化或其他翻译后修饰的位点。 了解蛋白质在细胞周期中特定时间的 PTM 总体非常重要。 这些信息无法从自下而上的策略中收集。 自上而下的蛋白质组学将用于检测蛋白质的大部分（>10kDa），以发现从细胞中分离的蛋白质过程中 PTM 的分布。 该工作流程将通过研究 Chk2 来开发，Chk2 是一种具有 30 多个磷酸化位点的自磷酸化激酶。 我们将绘制细胞周期中不同时间捕获的 Chk2 的磷酸化分布。