CHARACTERIZATION OF THE ROLE OF METAL IONS IN RNA FOLDING AND ACTIVATION

金属离子在 RNA 折叠和激活中的作用表征

• 批准号: 8362179
• 负责人: James E. Penner-Hahn
• 金额: $ 0.03万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2011
• 资助国家: 美国
• 起止时间: 2011-03-01 至 2012-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8362179
• 关键词: Adopted; Complex; DNA; Divalent Cations; Funding; Grant; Ions; Label; Metal Binding Site; Metals; National Center for Research Resources; Nature; Play; Principal Investigator; RNA; RNA Folding; Radiation; Research; Research Infrastructure; Resources; Role; Source; Structure; Testing; United States National Institutes of Health; Vertebral column; cost; inorganic phosphate; structural biology

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Divalent cations play a critical role in the folding and activation of RNA. Unlike DNA, where the canonical structure is well defined, RNAs are found to adopt a wide range of structures. In many cases, divalent cations (Mg2+, Mn2+, and Zn2+) are required for the RNA to adopt a biologically active structure. Recent NMR studies have provided direct evidence for a unique binding site of the metal to the RNA and have suggested that the metal forms an inner-sphere complex with the RNA. EXAFS will be used to test this proposal and to better define the nature of the RNA-metal interaction. In order to define specifically the RNA-metal binding site, thio-modified RNA will be used to label possible backbone phosphates.

该子项目是利用资源的众多研究子项目之一 由 NIH/NCRR 资助的中心拨款提供。子项目的主要支持 并且子项目的主要研究者可能是由其他来源提供的， 包括其他 NIH 来源。 子项目可能列出的总成本 代表子项目使用的中心基础设施的估计数量， NCRR 赠款不直接向子项目或子项目工作人员提供资金。 二价阳离子在 RNA 的折叠和激活中发挥着关键作用。与 DNA 不同，RNA 的典型结构是明确定义的，而 RNA 则采用多种结构。在许多情况下，RNA 需要二价阳离子（Mg2+、Mn2+ 和 Zn2+）才能形成生物活性结构。最近的核磁共振研究为金属与 RNA 的独特结合位点提供了直接证据，并表明金属与 RNA 形成内球复合物。 EXAFS 将用于测试该提议并更好地定义 RNA-金属相互作用的性质。为了具体定义 RNA-金属结合位点，硫代修饰的 RNA 将用于标记可能的主链磷酸盐。