Super Resolution PALM/STORM Microscopy System In Multi-User Facility

多用户设施中的超分辨率 PALM/STORM 显微镜系统

• 批准号: 8640512
• 负责人: Vladislav Verkhusha
• 金额: $ 50.12万
• 依托单位: ALBERT EINSTEIN COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-01 至 2015-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8640512
• 关键词: Architecture; Biological; Biomedical Research; Centrosome; Collaborations; Communities; Development; Disseminated Malignant Neoplasm; Funding; Grant; Image; Interdisciplinary Study; Lead; Macromolecular Complexes; Medicine; Microscope; Microscopy; Neurons; Nuclear; Organelles; Principal Investigator; Protein Engineering; Proteins; Publications; Research; Research Personnel; Resolution; Scientist; Sorting - Cell Movement; Structure; Subcellular structure; System; Techniques; Technology; Toxoplasma gondii; United States National Institutes of Health; Virus Assembly; cancer cell; college; commercialization; design; fluorescence microscope; instrumentation; light microscopy; messenger ribonucleoprotein; programs

DESCRIPTION (provided by applicant): The Analytical Imaging Facility (AIF) of the Albert Einstein College of Medicine supports NIH-funded investigators by giving access to state-of-the-art microscopy technologies that enhance collaborative, multidisciplinary research. This application is a shared instrumentation grant from the AIF to acquire a PALM/STORM (PALM and STORM are used interchangeably in this application since they are the same technology at the microscope interface) super-resolution system, a new microscopy system that uses photo activation localization microscopy to achieve super-resolution. This technology will have a high impact on the biomedical research at Einstein and will expand the scope of NIH-funded projects at Einstein. While most microscopy facilities have traditionally been equipped with wide-field, confocal, and multiphoton fluorescence microscopes, the resolution of the images acquired on these systems has been limited by the diffraction limit of the light microscopy. Recently, there have been major advances in the development of super-resolution light microscopy techniques, allowing scientists to move beyond the previous resolution limit and achieve a ten-fold improvement in resolution. The recent commercialization of the PALM/STORM technology is revolutionizing our understanding of the cellular organization by allowing scientists to study details in structures previously thought impossible to achieve by light microscopy. This technology is paving the way for asking refined questions about the organization of proteins in macromolecular complexes, organelles and subcellular structures. We anticipate that this PALM/STORM technology will greatly enhance and accelerate many NIH funded projects at Einstein, many of which are ideal for this application and would greatly benefit from access to this technology. In particular, this technology will be used to study diverse aspects of biological structures that include: formation of invasive structures in metastatic cancer cells (Dr. Condeelis, project #2), organization of podosome structures (Dr. Cox, project #3), virus assembly and budding (Dr. Kielian, project #4), nuclear architecture in T. gondii. (Dr. Kim, project #5), protein sorting in the transGolgi network (Dr. Muesch, project #6), structure and function of the centrosome (Dr. Sharp, project #7), and mRNP assembly in neurons (Dr. Singer, project #8). The Principal Investigator of this application, Dr. Vladislav Verkhusha (project #1), s one of the leaders in the field of fluorescent protein engineering and development, with a long publication record in this field. He has specifically designed many fluorescent proteins for exclusive use in the PALM technology. Since there is currently no PALM/STORM system available at Einstein, he has had to engage in lengthy, multi- state collaborations in order to advance his research. Acquisition of a PALM/STORM system will greatly facilitate his ongoing NIH-funded research program, to the benefit of the Einstein community as well as the scientific community at large. Lastly, we anticipate that the acquisition of a PALM/STORM system will lead to new collaborations and funding, and will stimulate new directions of scientific inquiry.

描述（由申请人提供）：阿尔伯特·爱因斯坦医学院的分析成像设施 (AIF) 通过提供最先进的显微镜技术来支持 NIH 资助的研究人员，从而增强协作性、多学科研究。此应用程序是 AIF 的共享仪器拨款，用于获取 PALM/STORM（PALM 和 STORM 在本应用程序中可互换使用，因为它们在显微镜接口处采用相同的技术）超分辨率系统，这是一种使用光激活的新型显微镜系统定位显微镜以实现超分辨率。这项技术将对爱因斯坦的生物医学研究产生重大影响，并将扩大美国国立卫生研究院资助的爱因斯坦项目的范围。虽然大多数显微镜设施传统上都配备了宽视场、共焦和多光子荧光显微镜，但在这些系统上获取的图像的分辨率受到光学显微镜衍射极限的限制。最近，超分辨率光学显微镜技术的发展取得了重大进展，使科学家能够超越之前的分辨率极限，实现分辨率提高十倍。 PALM/STORM 技术最近的商业化正在彻底改变我们对细胞组织的理解，科学家们可以研究以前认为无法通过光学显微镜实现的结构细节。这项技术为提出有关大分子复合物、细胞器和亚细胞结构中蛋白质组织的精细问题铺平了道路。我们预计这种 PALM/STORM 技术将极大地增强和加速爱因斯坦的许多 NIH 资助项目，其中许多项目非常适合这种应用，并将大大受益于这项技术。特别是，该技术将用于研究生物的各个方面 结构包括：转移性癌细胞中侵袭性结构的形成（Condeelis 博士，项目 #2）、足体结构的组织（Cox 博士，项目 #3）、病毒组装和出芽（Kielian 博士，项目 #4）、弓形虫的核结构。 （Kim 博士，项目 #5）、反高尔基体网络中的蛋白质分类（Muesch 博士，项目 #6）、中心体的结构和功能（Sharp 博士，项目 #7）以及神经元中的 mRNP 组装（Dr. Muesch，项目 #7）。歌手，项目#8）。该应用的首席研究员 Vladislav Verkhusha 博士（项目#1）是荧光蛋白工程和开发领域的领导者之一，在该领域拥有长期的发表记录。他专门设计了许多专用于 PALM 技术的荧光蛋白。由于爱因斯坦目前没有可用的 PALM/STORM 系统，他不得不进行长期的多州合作才能推进他的研究。购买 PALM/STORM 系统将极大地促进他正在进行的 NIH 资助的研究项目，造福爱因斯坦社区以及整个科学界。最后，我们预计 PALM/STORM 系统的收购将带来新的合作和资金，并将刺激科学探究的新方向。